IN-VITRO ANTIBACTERIAL, ANTIFUNGAL, ANTIBIOFILM, AND ANTIOXIDANT POTENTIALS OF ISOPIMPINELLIN RECOVERED FROM CITRULLUS COLOCYNTHIS
Keywords:
Keywords, Citrullus colocynthis, Isopimpinellin, Antibacterial, Antifungal, Antibiofilm, AntioxidantAbstract
Objective: The novel molecules recovered from traditional medicinal plants have various biological applications with fewer side effects compared to the synthetic molecules. In this regards, the present study aimed to investigate the antibacterial, antifungal, anti-biofilm and antioxidant properties of novel metabolite isolated from the seeds of Citrullus colocynthis.
Methods: The ethyl acetate (EA) extract of C. colocynthis seed was mixed with a different proportion of hexane and Silica gel column packed for the collection of different fractions. The active fractions were checked for its purity by thin layer chromatography and HPLC. The identified single molecule was further characterized using IR, 1H NMR, 13C NMR and mass spectral analysis. The minimum inhibitory concentration (MIC) of the compound was determined using broth microdilution method. A fungal biomass inhibition effect, the anti-biofilm and anti-oxidant property of the compound was evaluated.
Results: Purification of the EA extract by Silica gel column guided to identify active antimicrobial metabolite containing fractions. Further separation technique and spectroscopic analysis lead to confirm the novel molecule isopimpinellin with the molecular formula C13H10O5. The identified metabolite revealed promising antimicrobial activity against Bacillus subtilis (100 µg/ml) and Klebsiella pneumoniae (75 µg/ml); whereas Aspergillus niger and Candida albicans documented the MIC values of 150 µg/ml and 75 µg/ml respectively. Fungal biomass inhibition effect study further proved the antifungal activity of the compounds against the filamentous fungal strains. In addition, the compound showed promising anti-biofilm activity against the uropathogenic strain such as Escherichia coli and Pseudomonas aeruginosa. Interestingly the antioxidant properties of the compound were an advantage. Both anti-biofilm and antioxidant activities were dose dependent.
Conclusion: Novel isopimpinellin with different biological activities such as antibacterial, antifungal, antibiofilm and antioxidant properties would be helpful in minimizing the spreading of pathogenic microorganisms or preventing stress-related diseases.
Keywords: Citrullus colocynthis, Isopimpinellin, Antibacterial, Antifungal, Antibiofilm, Antioxidant
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