• Darshan Dharajiya Department of Plant Molecular Biology and Biotechnology, C. P. College of Agriculture, Sardarkrushinagar Dantiwada Agricultural University, Sardarkrushinagar-385506, Gujarat, India
  • Hitesh Jasani
  • Tarun Khatrani
  • Manthan Kapuria
  • Karen Pachchigar
  • Payal Patel


Antimicrobial activity, Phytochemical analysis, TLC bioautography, Minimum Inhibitory Concentration (MIC), Trigonella foenum-graecum


Objective: The present investigation was performed to evaluate the antimicrobial potential of Trigonella foenum-graecum leaves extracts and to elucidate the presence of phytochemicals responsible for its biological activity.

Methods: The plant extracts were prepared by sequential cold maceration method by using four solvents viz., hexane, ethyl acetate, methanol and distilled water. Antimicrobial activity of extracts was carried out by agar well diffusion method against four bacteria and five fungi. Minimum inhibitory concentration (MIC) of different extracts was determined using the broth dilution method. Thin layer chromatography (TLC), TLC bioautography and phytochemicals analysis were also performed.

Results: The antibacterial activity of T. foenum-graecum leaves extracts was found maximum on Serratia marcescens with a zone of inhibition (ZOI) of 12.33±0.57 mm by aqueous extract followed by inhibition of Bacillus cereus (ZOI = 11.50±0.50 mm) by the methanol extract. The range of MIC of different extracts recorded was 6.25 to 25 mg/ml. Results of the antifungal activity showed that methanol extract showed a maximum zone of inhibition against Trichoderma viridae (ZOI = 14.5±0.5 mm) followed by ethyl acetate extract (ZOI = 12.0±1.0 mm). The MIC value for methanol extract against T. viridae was 6.25 mg/ml. The result of TLC bioautography revealed that compounds eluted at Rf 0.58 and Rf 0.67 exhibited strong while compounds eluted at Rf 0.31 and Rf 0.37 showed moderate antibacterial activity against S. marcescens. Phytochemicals analysis indicated that methanol and aqueous extracts contain more phytochemicals as compared to hexane and ethyl acetate extracts.

Conclusion: The results obtained in this study clearly indicate that T. foenum-graecum leaves extracts to have a significant potential to be used as an antimicrobial agent due to the presence of different phytochemicals.

Keywords: Antimicrobial activity, Phytochemicals analysis, TLC bioautography, Minimum Inhibitory Concentration (MIC), Trigonella foenum-graecum



Download data is not yet available.


Teshager T, Herrero IA, Porrero MC, Garde J, Moreno MA, DomıÌnguez L. Surveillance of antimicrobial resistance in Escherichia coli strains isolated from pigs at Spanish slaughterhouses. Int J Antimicrob Agents 2000;15:137-42.

Cohen ML. Changing patterns of infectious disease. Nature 2000;406:762-7.

Shrikanth VM, Janardhan B, Dhananjaya BL, Muddapura UM, More SS. Antimicrobial and antioxidant activity of methanolic root extract of Tabernaemontana alternifolia L. Int J Pharm Pharm Sci 2015;7:66-9.

Raghunath D. Emerging antibiotic resistance in bacteria with special reference to India. J Biosci 2008;33:593-603.

Dhayalan M, Jegadeeshwari A, Gandhi N. Biological activity sources from traditionally used tribe and herbal plants material. Asian J Pharm Clin Res 2015;8:11-23.

Rai M, Acharya D, Wadegaonkar P. Plant derived-antimycotics; potential of Asteraceous plants. Plant-derived antimycotics. Current trends and future prospects. Hartworth Press. NY; 2003. p. 165-95.

Dash BK, Sultana S, Sultana N. Antibacterial activities of methanol and acetone extracts of fenugreek (Trigonella foenum) and coriander (Coriandrum sativum). Life Sci Med Res 2011;27:1-8.

Snehlata HS, Payal DR. Fenugreek (Trigonella foenum-graecum L.): an overview. Int J Curr Pharm Rev Res 2012;2:169-87.

Syeda BB, Muhammad IB, Shahabuddin M. Antioxidant activity from the extract of fenugreek seeds. Pak J Anal Environ Chem 2008;9:78-83.

Petropoulos GA. Fenugreek-The genus Trigonella, Taylor and Francis, London and New York; 2002.

Basch E, Ulbricht C, Kuo G, Szapary P, Smith M. Therapeutic applications of fenugreek. Altern Med Rev 2003;8:20-7.

Xue WL, Li XS, Zhang J, Liu YH, Wang ZL, Zhang RJ. Effect of Trigonella foenum-graecum (fenugreek) extracts on blood glucose, blood lipid and hemorheological properties in streptozotocin-induced diabetic rats. Asia Pac J Clin Nutr 2007;16(Suppl 1):422-6.

Karim A, Sohail MN, Munir S, Sattar S. Pharmacology and phytochemistry of Pakistani herbs and herbal drugs used for the treatment of diabetes. Int J Pharmacol 2011;7:419-39.

Shabbeer S, Sobolewski M, Anchoori RK, Kachhap S, Hidalgo M, Jimeno A, et al. Fenugreek: a naturally occurring edible spice as an anticancer agent. Cancer Biol Ther 2009;8:272-8.

Subhashini N, Nagarajan G, Kavimani S. Anti-inflammatory and in vitro antioxidant property of Trigonella foenum graecum seeds. J Pharmacol Toxicol 2011;6:371-80.

Kaviarasan S, Naik GH, Gangabhagirathi R, Anuradha CV, Priyadarsini KI. In vitro studies on antiradical and antioxidant activities of fenugreek (Trigonella foenum graecum) seeds. Food Chem 2007;103:31-7.

Yasmin H, Kaisar MA, Sarker MMR, Rahman MS, Rashid MA. The preliminary anti-bacterial activity of some indigenous plants of Bangladesh. Dhaka Univ J Pharm Sci 2009;8:61-5.

Randhir R, Lin YT, Shetty K. Phenolics, their antioxidant and antimicrobial activity in dark germinated fenugreek sprouts in response to peptide and phytochemical elicitors. Asia Pac J Clin Nutr 2004;13:295-307.

Abdel-Massih R, Abdou E, Baydoun E, Daoud Z. Antibacterial activity of the extracts obtained from Rosmarinus officinalis, Origanum majorana, and Trigonella foenum-graecum on highly drug-resistant gram-negative bacilli. J Bot 2010. Doi.org/ 10.1155/2010/464087. [Article in Press]

Nahar N, Rahman MS, Rahman SM, Moniruzzaman M. GC-MS analysis and antibacterial activity of Trigonella foenum-graecum against bacterial pathogens. Free Radicals Antioxid 2016;6:109-14.

Haouala R, Hawala S, El-Ayeb A, Khanfir R, Boughanmi N. Aqueous and organic extracts of Trigonella foenum-graecum L. inhibit the mycelia growth of fungi. J Environ Sci 2008;20:1453-7.

Mitra A, Bhattacharya D. Dose-dependent effects of Fenugreek composite in Diabetes with dislipidaemia. Internet J Food Safety 2006;8:49-55.

Gowda TSS, Dinesha R, Harsha R, Leela S. Free radical scavenging activity of lutein–isolated from methi leaves (Trigonella foenum graecum). Int J Pharm Pharm Sci 2010;2:113-7.

Mandal P, Gupta SK. Improvement of antioxidant activity and related compounds in fenugreek sprouts through nitric oxide priming. Int J Pharm Sci Rev Res 2014;26:249-57.

Rahman A, Parvez MAK, Parvin T, Paul DK, Sattar MA. Antimicrobial activity of three bioactive compounds from the stream bark of Piper chaba hunter. Biosci Res 2004;1:16-20.

Dharajiya D, Patel P, Patel M, Moitra N. In vitro antimicrobial activity and qualitative phytochemical analysis of Withania somnifera (L.) Dunal extracts. Int J Pharm Sci Rev Res 2014;27:349-54.

Thakur S, Tiwari KL, Jadhav SK. Antibacterial screening of root extract of Asparagus racemosus Willd. Curr Trends Biotechnol Pharm 2015;9:147-50.

Dharajiya D, Khatrani T, Patel P, Moitra N. Evaluation of antifungal activity of Emblica officinalis, Aloe vera and Vitex negundo extracts. J Chem Biol Phys Sci 2015;5:3990-6.

Gupta PC, Batra R, Chauhan A, Goyal P, Kaushik P. Antibacterial activity and TLC bioautography of Ocimum basilicum L. against pathogenic bacteria. J Pharm Res 2009;2:407-9.

Ishnava KB, Chauhan JB, Garg AA, Thakkar AM. Antibacterial and phytochemical studies on Calotropis gigantia (L.) R. Br. latex against selected cariogenic bacteria. Saudi J Biol Sci 2012;19:87-91.

Dharajiya D, Patel P, Moitra N. Antibacterial activity of Emblica officinalis (Gaertn.) Fruits and Vitex negundo (L.) Leaves. Curr Trends Biotechnol Pharm 2015;9:357-68.

Iwu MW, Duncan AR, Okunji CO. New antimicrobials of plant origin. Perspectives on new crops and new uses. Alexandria, VA: ASHS Press; 1999. p. 457-62.

Martins S, Amorim EL, Sobrinho TJP, Saraiva AM, Pisciottano MN, Aguilar CN, et al. Antibacterial activity of crude methanolic extract and fractions obtained from Larrea tridentata leaves. Ind Crops Prod 2013;41:306-11.

Duraipandiyan V, Ayyanar M, Ignacimuthu S. Antimicrobial activity of some ethnomedicinal plants used by a paliyar tribe from Tamil Nadu, India. BMC Complementary Altern Med 2006;6:35.

Yadav R, Tiwari R, Chowdhary P, Pradhan CK. A pharmacognostical monograph of Trigonella foenum-graecum seeds. Int J Pharm Pharm Sci 2011;3:442-5.



How to Cite

Dharajiya, D., H. Jasani, T. Khatrani, M. Kapuria, K. Pachchigar, and P. Patel. “EVALUATION OF ANTIBACTERIAL AND ANTIFUNGAL ACTIVITY OF FENUGREEK (TRIGONELLA FOENUM-GRAECUM) EXTRACTS”. International Journal of Pharmacy and Pharmaceutical Sciences, vol. 8, no. 4, Apr. 2016, pp. 212-7, https://journals.innovareacademics.in/index.php/ijpps/article/view/10693.



Original Article(s)