EVALUATION OF DIURETIC AND ANTIUROLITHIATIC PROPERTIES OF ETHANOLIC EXTRACT OF SIDA ACUTA BURM. F. IN WISTAR ALBINO RATS
Keywords:
Zinc disc implantation, calculi, diuretic index, CystoneAbstract
Objective: To evaluate the diuretic and antiurolithiatic properties of ethanolic extract of Sida acuta Burm. f. in albino rats.
Methods: Three different doses of Sida acuta (100 mg/kg, 200 mg/kg, 400 mg/kg) were tested for diuretic activity and compared with furosemide (25 mg/kg) and control group. 24 hr urine output and urinary concentrations of sodium (Na+), potassium (K+) and chloride (Cl-) ions were the parameters measured. Prophylactic and curative antiurolithiatic activity was tested for three doses (100 mg/kg, 200 mg/kg & 400 mg/kg) of Sida acuta and compared with control group and animals treated with standard herbal preparation Cystone (750 mg/kg) using zinc disc implantation model.
Results: Sida acuta (400 mg/kg) significantly increased the urine output (p<0.001), which was almost equivalent to furosemide. Sida acuta also caused a significant increase in the excretion of K+ and Cl-at higher doses (200 mg/kg, 400 mg/kg). Effects on Na+excretion were not significant for all three doses of the test drug. Sida acuta (200 mg/kg, 400 mg/kg) showed statistically significant (p<0.05) reduction in depositions around zinc discs as compared to control when tested in prophylactically treated rats, but not in rats treated post-urolithiasis induction.
Conclusion: Sida acuta produced a dose-dependent increase in urine output, with no effect on sodium excretion. This probably indicates that the diuresis produced could be due to its aquarectic action. It also showed significant prophylactic antiurolithiatic activity, but further studies are necessary to evaluate fully its therapeutic potential.
Keywords: Zinc disc implantation, Calculi, Diuretic index, Cystone
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Shirfule AL, Racharla V, Quadri SSYH, Khandare AL. Exploring antiurolithiatic effects of Gokshuradi polyherbal ayurvedic formulation in ethylene-glycol-induced urolithic rats. J Evidence-Based Complementary Altern Med 2013. Doi:10.1155/2013/763720. [Article in Press]
López M, Hoppe B. History, epidemiology and regional diversities of urolithiasis. Pediatr Nephrol 2010;25:49-59.
Butterweck V, Khan SR. Herbal medicines in the management of urolithiasis: alternative or complementary? Planta Med 2009;75:1095-103.
Kelley JM. Extracorporeal shock wave lithotripsy of urinary calculi. Theory, efficacy, and adverse effects. West J Med 1990;153:65-9.
Moe OW, Pearle MS, Sakhaee K. Pharmacotherapy of urolithiasis: evidence from clinical trials. Kidney Int 2011;79:385-92.
Semins MJ, Matlaga BR. Medical evaluation and management of urolithiasis. Ther Adv Urol 2010;2:3-9.
Mohideen S, Sasikala E, Gopal V. Pharmacognostic studies on Sida acuta Burm. f. Anc Sci Life 2002;22:57-66.
Chopra RN, Nayar SL, Chopra IC. Glossary of indian medicinal plants. New Delhi: Council of Scientific and Industrial Research; 1956. p. 226.
Vimala T, Gopalakrishnan S. Inhibitory effect of the root of Sida acuta Burm. f. on calcium oxalate crystal growth. J Res Edu Indian Med 2012;18:21-6.
Arafat OM, Tham SY, Sadikun A, Zhari I, Haughton PJ, Asmawi MZ. Studies on diuretic and hypouricemic effects of Orthosiphon stamineus methanol extracts in rats. J Ethnopharmacol 2008;118:354-60.
Cohanim M, Yendt ER. Reduction of urine oxalate during long-term thiazide therapy in patients with calcium urolithiasis. Invest Urol 1980;18:170-3.
Redfern J, Kinninmonth M, Burdass D, Verran J. Using soxhlet ethanol extraction to produce and test plant material (essential oils) for their antimicrobial properties. J Microbiol Biol Educ 2014;15:45-6.
Sreedevi CD, Latha PG, Ancy P, Suja SR, Shyamal S, Shine VJ, et al. Hepatoprotective studies on Sida acuta Burm. f. J Ethnopharmacol 2009;124:171-5.
Udupa AL, Sanjeeva, Benegal A, Prusty V, Kodancha GP, Kumar MCS, et al. Diuretic activity of Phyllanthus niruri (Linn.) in rats. Health 2010;2:511-2.
Vermeulen CW, Grove WJ, Goetz R, Ragins HD, Correll NO. Experimental urolithiasis. I. Development of calculi upon foreign bodies surgically introduced into bladders of rats. J Urol 1950;64:541-8.
Vyas N, Argal A. Antiurolithiatic activity of extract and oleanolic acid isolated from the roots of Lantana camara on zinc disc implantation induced urolithiasis. ISRN Pharmacol 2013. doi.org/10.1155/2013/951795. [Article in Press]
Reilly RF, Jackson EK. Regulation of renal function and vascular volume. In: Brunton LL, Chabner BA, Knollman BC. editors. Goodman and Gilman’s the pharmacological basis of therapeutics. 12th ed. New York: The McGraw-Hill Companies; 2011. p. 671-719.
Kumar M, Bussmann RW, Joshi M, Kumar P. Ethnomedicinal uses of plants close to rural habitation in Garhwal Himalaya, India. J Med Plants Res 2011;5:2252-60.
Asif M, Atif M, Malik ASA, Dan ZC, Ahmad I, Ahmad A. Diuretic activity of Trianthema portulacastrum crude extract in albino rats. Trop J Pharm Res 2013;12:967-72.
Szentmihályi K, Kéry Ã, Then M, Lakatos B, Sándor Z, Vinkler P. Potassium-sodium ratio for the characterization of medicinal plant extracts with diuretic activity. Phytother Res 1998; 12:163â€6.
Hook I, Mcgee A, Henman M. Evaluation of dandelion for diuretic activity and variation in potassium content. Pharm Biol 1993;31:29â€34.
Awang DVC. Kidney, urinary tract, and prostate problems. In: Awang DVC, Robbers JE. editors. Tyler’s Herbs of choice: the therapeutic use of phytomedicinals. 3rd ed. Boca Raton: CRC Press; 2009. p. 59-76.
Vogel HG. Drug discovery and evaluation: pharmacological assays. 3rd ed. Berlin: Springer-Verlag. Chapter C, Activity on urinary tract; 2008. p. 457–510.
Khan SR. Animal models of kidney stone formation: an analysis. World J Urol 1997;15:236-43.
Singh PK, Patil CR, Harlalka GV, Gaud NP. Zinc disc implantation model of urinary bladder calculi and humane endpoints. Lab Anim 2010;44:226-30.
Pearle MS, Lotan Y. Urinary lithiasis: etiology, epidemiology, and pathogenesis. In: Wein AJ, Kavoussi LR, Novick AC, Partin AW, Peters CA. editors. Campbell-Walsh Urology. 10th ed. Philadelphia: Saunders-Elsevier; 2012. p. 1257-86.
Miller C, Kennington L, Cooney R, Kohjimoto Y, Cao LC, Honeyman T, et al. Oxalate toxicity in renal epithelial cells: characteristics of apoptosis and necrosis. Toxicol Appl Pharmacol 2000;162:132-41.
Londonkar RL, Patil SJ, Patil SB. Phytochemical and contraceptive property of Sida acuta Burm Fi. iin. in albio rats. Int J PharmTech Res 2009;1:1260-6.
Pietta PG. Flavonoids as antioxidants. J Nat Prod 2000; 63:1035-42.
Kähkönen MP, Heinonen M. Antioxidant activity of anthocyanins and their aglycons. J Agric Food Chem 2003;51:628-33.