EFFECT OF C-GLYCOSYL FLAVONE FROM URGINEA INDICA ON ANTIBIOTIC INDUCED MICROBIAL CELL DEATH
Keywords:
Antimicrobial, synergism, cytotoxicity, cytoplasmic membrane damage and cell deathAbstract
Objective: The aim of the present study was to isolate and characterize C-glycosyl flavone from Urgenia. indica bulb and to evaluate its effect on microbial cell growth.
Methods: Methanolic extract of U. indica bulb was prepared using Soxhlation. Isolation was performed using silica gel column chromatography, and characterization was done based on IR, NMR and mass spectral data. The effect of C-glycosyl flavone was evaluated on microbial growth inhibition C-glycosyl flavone in terms of cytotoxicity, cell membrane damage, and cell death.
Results: The results indicates that O-glycosyl flavanone (5,41-di hydroxyl-31-methoxy-7-O-[(α-L-rhamnosyl-(1[11]1-6[11])-β-D-glucopyranosyl)] flavanone), O-glycosyl flavone (5,41-dihydroxy-31-methoxy-7-O-β-D-glucopyranosyl flavone) and C-glycosyl flavone (5,7-dihydroxy-2-[41-hydroxy-31-(methoxymethyl) phenyl]-6-C-β-glucopyranosyl flavones) showed significantly sensitivity against Staphylococcus aureus, Bacillus subtilis, Rhizopus oryzae and Aspergillus niger. Synergism of C-glycosyl flavone on antibacterial activity of ciprofloxacin with the FIC index of 0.3 against S. aureus and 0.5 against B. subtilis. Antifungal activity of clotrimazole with the FIC index 0.3 against R. oryzae and 0.48 against A. niger. C-glycosyl flavone increased the ciprofloxacin-induced cytotoxicity from 63 to 91% against S. aureus and 56 to 89% against B. subtilis, whereas clotrimazole is induced cytotoxicity from 36 to 49% against R. oryzae and 23 to 41% against A. niger. C-glycosyl flavone increased the ciprofloxacin-induced cell death in S. aureus and B. subtilis and clotrimazole induced cell death in R. oryzae and A. niger as evident by propidium iodide staining, Tunel positive cells, and cytoplasmic membrane damage.
Conclusion: The present investigation provides scientific and rationalism for the folkloric use of U. indica as an antimicrobial agent.
Keywords: Antimicrobial, Synergism, Cytotoxicity, Cytoplasmic membrane damage and cell death
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