PHYTOCHEMICAL SCREENING AND ANTIMICROBIAL ACTIVITY OF LEUCAS MARRUBIOIDES DESF. ROOT EXTRACTS
DOI:
https://doi.org/10.22159/ijpps.2016v8i12.11837Keywords:
Leucas marrubioides Desf, Antimicrobial activity, Agar well diffusion, MICAbstract
Objective: The number of multidrug-resistant microbial strains and the appearance of strains with reduced susceptibility to antibiotics forced scientists to search for new antimicrobial agents from plants. The present study was aimed to evaluate the phytochemical constituents and antimicrobial potential of Leucas marrubioides Desf. root extracts.
Methods: The roots of L. marrubioides was extracted with solvents of varying polarity, namely pet-ether, chloroform, and methanol. The phytochemical screening was carried out qualitatively to identify the major phytoconstituents present in the extracts. The antimicrobial activity of extracts was evaluated against five Gram-positive bacteria, five Gram-negative bacteria and six fungal strains by agar well diffusion method. The Minimum Inhibitory Concentration (MIC) of the extracts was determined by Broth dilution method.
Results: The results of the study indicated that the pet-ether and chloroform extracts of the plant were highly effective towards most of the Gram-positive, Gram-negative bacteria and fungal strains. While, the methanol extract was moderately active towards all the tested organisms. The pet ether extract had lowest MIC of 1.562 mg/ml against V. cholerae and Curvularia sp and chloroform extract had lowest MIC of 1.562 mg/ml against only C. neoformans. The methanol extract showed MIC of 1.562 mg/ml against the organisms S. pyogenes, C. neoformans and C. albicans.
Conclusion: The potent antimicrobial activity against Gram-positive bacteria, Gram-negative bacteria and fungal strains may be due to the presence of broad spectrum antibiotic compound in the extracts. The study validates the use of the plant in the treatment of bacterial and fungal infections.
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Sobia N, Yamin B, Abdul W, Muhammad Z, Sadia S, Sabbir A, et al. Evaluation of anticancer activity of Debregeasia Salicifolia extract against estrogen receptor positive cell line. Afr J Biotechnol 2011;10:990-5.
Chandra M. Antimicrobial activity of medicinal plants against human pathogenic bacteria. Int J Biotechnol Bioengineering Res 2013;4:653-8.
Laila FN, Meqdam MM, Alkofahi A. Antibacterial activity of jordanian medicinal plants. Pharm Biol 1999;37:196-201.
Ahmad I, Aqil F. In vitro efficacy of bioactive extracts of 15 medicinal plants against ESbL-producing multidrug-resistant enteric bacteria. Microbiol Res 2007;162:264-75.
Barbour EK, Sharif M, Sagherian VK, Habre AN, Talhouk RS, Talhouk SN. Screening of selected indigenous plants of Lebanon for antimicrobial activity. J Ethnopharmacol 2004;93:1-7.
Iwu MW, Duncan AR, Okunji CO. New antimicrobials of plant origin In: Perspectives on new Crops and new Uses. eds. J Janick, ASHS Press: Alexandria, VA; 1999. p. 457-62.
Gonzalez CE, Venzon D, Lee S, Mueller BU, Pizzo PA, Walsh TJ. Risk factors for fungemia in children infected with human immunodeficiency virus: a case-control study. Clin Infect Dis 1996;23:515-21.
Rajesh D, Amita G, Mandal TK, Desh DS, Vivek B, Gurav AM, et al. Antimicrobial activity of some Indian medicinal plants. Afr J Trad CAM 2007;4:313-8.
Sankar KD, Debdulal B, Sourav C, Krishnendu BK. Antimicrobial activities of some medicinal plants of West Bengal. Int J Pharm Biol Sci 2010;1:1-10.
Monroe S, Polk R. Antimicrobial use and bacterial resistance. Curr Opin Microbiol 2000;3:496-501.
Gowrish A, Vagdevi HM, Rajashekar H. In vitro antioxidant and antitubercular activity of Leucas marrubioides Desf. root extracts. J Appl Pharm Sci 2015;5:137-42.
Gowrish A, Vagdevi HM, Rajashekar H, Vijaya Kumar ML, Shobha KS. In vitro cytotoxic and antioxidant activity of Gnidia glauca (Fresn Gilg) root extract. J Appl Chem 2013;2:1362-9.
Parekh J, Chanda SV. In vitro antimicrobial activity and phytochemical analysis of some Indian medicinal plants. Turkish J Biol 2007;31:53-8.
Antara S, Mala B, Evaluation of antimicrobial activity of different solvent extracts of medicinal plant: Melia Azedarach L. Int J Curr Pharm Res 2012;4:67-73.
Shanmugapriya K, Thayumanavan T, Jinu U, Senthil MT. Antimicrobial activity of methanolic extracts of indigenous traditional Indian folk medicinal plant, Gnaphalium polycaulon. Int J Green Pharm 2015;9:39-44.
Dewanjee SD, Kundu M, Maiti A, Majumdar R, Majumdar A, Mandal SC. In vitro evaluation of antimicrobial activity of crude extracts from plants Diospyros peregrine, Coccinia grandis and Swietenia macrophylla. Trop J Pharm Res 2007;6:773-8.
Marjorie MC. Plant products as antimicrobial agents. Clin Microbiol Rev 1999;12:564-82.
Doughari JH. Antimicrobial activity of Tamarindus indica Linn. Trop J Pharm Res 2006;5:597-603.
Srinivasan D, Perumalsamy LP, Nathan S, Sures T. Antimicrobial activity of certain Indian medicinal plants used in folkloric medicine. J Ethnopharm 2001;94:217-22.
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