CYTOTOXICITY AND APOPTOTIC INDUCIBILITY OF HYDRO-ETHANOLIC EXTRACT OF AERIAL PARTS OF POTHOS SCANDENS L. AGAINST MCF-7 BREAST CANCER CELL LINE
Keywords:
Apoptosis, comet assay, L929, MCF-7, MTT, Pothos scandens, 50 ethanolic extractAbstract
Objective: To investigate the ability of 50% hydro-ethanolic extract of Pothos scandens (P. scandens) to induce cytotoxicity and apoptosis in MCF-7 cell lines.
Methods: Aerial parts of P. scandens were extracted with 50% ethanol. The crude extract obtained was evaluated for its ability to induce cytotoxicity against MCF-7 (breast cancer) and L929 (normal fibroblast) cell lines by MTT (3-(4, 5 dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide) assay. The ability to induce apoptosis was determined by acridine orange (AO) and ethidium bromide (EB) double staining method. The above activity was confirmed using the comet assay.
Results: The study revealed that the plant extract showed significant cytotoxic activity against MCF-7 cell lines with an IC50 of 90.18±5.20 µg/ml. Further it was also found that the cell death of MCF-7 treated with crude extract was due to the induction of apoptosis, which was ascertained by comet assay.
Conclusion: The study provides scientific evidence for the anticancer activity of extracts of P. scandens paving a way to further research of using this plant in the development of the novel anticancer drug.
Keywords: P. scandens, MCF-7, L929, MTT assay, Apoptosis, Comet assay
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References
Mathers CD, Boschi-Pinto C, Lopez AD, Murray CJL. Cancer incidence, mortality and survival by site for 14 regions of the world. World Health Organization; 2001. p. 3.
Gerald Karp. Cell and molecular biology. 3rd Edition. John Wiley and Sons, Inc; 2003. p. 671-702.
Smigal C, Jemal A, Ward E, Cokkinides V, Smith R, Howe H, et al. Trends in breast cancer by race and ethnicity: Update. CA Cancer J Clin 2006;56:106-30.
Russo J, Yang X, Hu Y. Biological and molecular basis of human breast cancer. Front Biosci 1998;3:944-60.
Guo J, Wang MH. Extract of Ulmus davidiana planch barks induced apoptosis in human hepatoma cell line HEPG2. Exp Clin Sci J 2009;8:130-7.
Saalu LC, Ajayi GO, Adeneye AA, Imosemi IO, Osinubi AA. Ethanol seed extract of grapefruit (Citrus paradisi) as an effective attenuator of doxorubicin-induced oxidative stress in the rat heart. Int Cancer Res 2009;5:44-52.
English DR, Armstrong BK, Kricker A, Fleming C. Sunlight and cancer. Cancer Causes Control 1997;8:271-83.
Kuper H, Boffetta P, Adami HO. Tobacco use and cancer causation: association by tumour type. J Intern Med 2002;252:206-24.
Jeyachandran RA, Mahesh, Cindrella L. DEN-induced cancer and its alleviation by Anisomeles malabarica L R. Br. ethanolic leaf extract in male albino mice. Int J Cancer Res 2007;3:174-9.
Capua CJ, Hopson NP, Stewart CMM, Johnston GR, Neill KLO, Schaalje GB, et al. Cytotoxicity of Atriplex confertifolia. J Toxicol 2010;1155:1-7.
George S, Bhalerao SV, Lidstone EA, Ahmad IS, Abbasi A, Cunningham BT, et al. Cytotoxicity screening of Bangladeshi medicinal plant extracts on pancreatic cancer cells. BMC Complementary Altern Med 2010;10:52.
Sangwoo L, Chunjie X, Shengji P. Ethnobotanical survey of medicinal plants at periodic markets of honghe prefecture in Yunnan Province, SW China. J Ethnopharmacol 2008; 117:362-77.
Baharuddin S, Mansor M. Medicinal aroids conservation: a case study of a floral garden. School of Biological Scie Ces, Universiti Sains Malayasia. Proceedings of the 4th IMT-GT UNINET Conference; 2002. p. 216-9.
Angkhana I, Shengji P, Balslev H, Wong PW, Trisonthi C. A comparative study on medicinal plants used in Akha's traditional medicine in China and Thailand, cultural coherence or ecological divergence? J Ethnopharmacol 2008;116:508-17.
Enos Tangke Arung, Britanto Dani Wicaksono, Yohana Ayupriyanti Handoko, Irawan Wijaya Kusuma, Dina Yulia, Ferry Sandra. Anti-cancer properties of diethyl ether extract of wood from Sukun (Antocarpus altilis) in human breast cancer (T47D) cells). Trop J Pharm Res 2009;8:217-324.
Zhang JH, YU J, Li WX, Cheng CP. Evaluation of Mn2+ stimulated, and Zn2+ inhibited apoptosis in rat corpus luteal cells by flow cytometry and fluorochromes staining. Chin J Physiol 1998;41:121-6.
Singh NP, McCoy MT, Tice RR, Schneider EL. A simple technique for quantitation of low levels of DNA damage in individual cells. Exp Cell Res 1988;175:184–91.
Smit HF, Woerdenbag HJ, Singh RH, Meulenbeld GH, Labadie RP, Zwaving JH. Ayurvedic herbal drugs with possible cytostatic activity. J Ethnopharmacol 1995;47:75-84.
R and D Systems: Cytokine Bulletin: Cytochine-Related Mechanisms of Apoptosis, R and D Systems, Minneapolis, MN; 1997.
Kerr JFR, Winterford CM, Harmon BV. Apoptosis. It's significance in cancer and cancer therapy. Cancer 1994;73:2013-26.
Sadegh Fattahi, Ali Motevalizadeh Ardekani, Ebrahim Zabihi, Zeinab Abedian, Amrollah Mostafazadeh, Roghayeh Pourbagher, et al. Antioxidant and apoptotic effects of an aqueous extract of Urtica dioica on the MCF-7 human breast cancer cell line. Asian Pacific J Cancer Prevention 2013;14:5317-23.
Dhawan A, Bajpayee M, Parmar D. Comet assay a reliable tool for the assessment of DNA damage in different models. Cell Biol Toxicol 2009;25:5-32.
Cok I, UlutaÅŸ OK, OkuÅŸluk O, Durmaz E, Demir N. Evaluation of DNA damage in common carp (Cyprinus carpio L) by comet assay for determination of possible pollution in lake mogan (Ankara). Sci World J 2011;11:1455-61.
Havsteen B. Commentary: flavonoids, a class of natural products of high pharmacological potency. Biochem Pharmacol 1983;32:1141-8.
Lalitharani, Mohan VR, Maruthupandian A. Pharmacognostical and phytochemical studies on Pothos scandens L. Int J Phytomed 2010;2:277-83.
Saurabh Gupta, Duraiswamy B, Satishkumar MN. Peritoneal mast cell stabilization potential of Pothos scandens L. Indian J Pharmacol 2013;45:83-6.