SCREENING OF CELLULOLYTIC BACTERIA FOR PRODUCING CELLULASE UNDER SOLID STATE FERMENTATION USING WATER HYACINTH AS A SUBSTRATE
Keywords:
Water hyacinth, Cellulase, Solid state fermentation, Optimization, PurificationAbstract
Objective: The infestation of water hyacinth in the aquatic system produces serious environmental problems. In our study, we have used the water hyacinth as a substrate for the production of cellulase enzyme from bacteria isolated from decayed water hyacinth.
Methods: The bacterial isolates were morphologically and biochemically characterized and the bacterial cultures were identified as Proteus vulgaris and Serratia ficaria respectively. The effect of various process parameters such as pH, temperature, substrate concentration and fermentation time on cellulase production by Proteus vulgaris were optimized.
Results: Out of twenty five isolates, two bacteria viz. AK1 and AK2 grew well on CMC agar media. Cellulase enzyme activity was found to be higher (4.07 U/ml) in case of Proteus vulgaris compared to Serratia ficaria. Cellulase production by Proteus vulgaris was optimized as pH 7, 35 °C temperature, 30% of water hyacinth and 24 h of incubation time. Cellulase produced under optimum condition was purified using dialysis and ion exchange chromatography. The molecular weight and specific activity of completely purified cellulase was found to be 33 KDa and 361.25 U/mg respectively.
Conclusion: It can be concluded that the water hyacinth can be used as a potential substrate for production of cellulase by Proteus vulgaris which is isolated from decayed water hyacinth.Downloads
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