A STUDY COMPARING THE ANORECTIC ACTIVITY OF HOT AND COLD AQUEOUS EXTRACTS OF DOLICHOS BIFLORUS LINN. SEEDS IN FREELY FEEDING RATS
Keywords:
Dolichos biflorus, anorectic, behavioral satiety sequence, food intakeAbstract
Objective: The aim of the study was to evaluate the anorectic activity of hot (pre-cooked under pressure) and cold (soaked overnight) aqueous extracts of the seeds of D. biflorus in freely feeding Wistar albino rat model.
Methods: Hot extract at a dose of 4 % w/v was given to the group 1 and cold extract at a dose of 4 % w/v was given to group 2 while group 3 was kept as the control group. Food intake and analysis of behavioral satiety sequence (BSS) was assessed after 4 w of extract administration whereas body weight was recorded both before and after treatment.
Results: Cold extract group showed significant anorectic activity (p<0.05) but not the hot extract group. The cold extract group, unlike the hot extract group also exhibited an acceleration of the BSS indicating that the anorectic activity is primary.
Conclusion: The study has thus brought out the potential appetite suppressant activity of the cold extract of D. biflorus seeds.
Keywords: Dolichos biflorus, Anorectic, Behavioral satiety sequence, Food intake
Downloads
References
Flegal KM, Carroll MD, Kit BK, Ogden CL. Prevalence of obesity and trends in the distribution of body mass index among US adults, 1999-2010. JAMA 2012;307:491-7.
Ogden CL, Carroll MD, Kit BK, Flegal KM. Prevalence of obesity and trends in body mass index among US children and adolescents, 1999-2010. JAMA 2012;307:483-90.
Must A, Spadano J, Coakley EH, Field AE, Colditz G, Dietz WH. The disease burden associated with overweight and obesity. JAMA 1999;282:1523-29.
Wanders AJ, Borne JJ, Graaf C, Hulshof T, Jonathan MC, Kristensen M, et al. Effects of dietary fibre on subjective appetite, energy intake and body weight: a systematic review of randomized controlled trials. Obes Rev 2011;12:724-39.
Muthu AK, Sethupathy S, Manavalan R, Karar PK. Hypolipidemic effect of methanolic extract of Dolichos biflorus Linn. in high-fat diet fed rats. Indian J Exp Biol 2005;43:522-5.
Sengupta K, Mishra AT, Rao MK, Sarma KVS, Krishnaraju AV, Trimurtulu G. Efficacy of an herbal formulation LI10903F containing Dolichos biflorus and Piper beetle extracts on weight management. Lipids Health Dis 2012;11:176.
Nanta R, Kale RK. Chemomodulatory effect of Dolichos biflorus Linn. on skin and forestomach papilloma genesis in swiss albino mice. Indian J Exp Biol 2011;49:483-90.
Halford JC, Wanninayake SC, Blundell JE. Behavioral satiety sequence (BSS) for the diagnosis of drug action on food intake. Pharmacol Biochem Behav 1998;61:159-68.
Halford JC, Harrold JA, Boyland EJ, Lawton CL, Blundell JE. Serotonergic drugs: effects on appetite suppression and use for the treatment of obesity. Drug 2007;67:27-55.
Kaur G, Kulkarni SK. Investigations on possible serotonergic involvement in effects of OB-200G (polyherbal preparation) on food intake in female mice. Eur J Nutr 2001;40:127-33.
Suzuki K, Simpson KA, Minnion JS, Shillito JC, Bloom SR. The role of gut hormones and the hypothalamus in appetite regulation. Endocr J 2010;57:359-72.
Andrews ZB, Liu ZW, Walllingford N, Erion DM, Borok E, Friedman JM, et al. UCP2 mediates ghrelin's action on NPY/AGRP neurons by lowering free radicals. Nature 2008;454:846-51.
Wren AM, Small CJ, Abbott CR, Dhillo WS, Seal LJ, Cohen MA, et al. Ghrelin causes hyperphagia and obesity in rats. Diabetes 2001;50:2540-7.
Wren AM, Seal LJ, Cohen MA, Brynes AE, Frost GS, Murphy KG, et al. Ghrelin enhances appetite and increases food intake in humans. J Clin Endocrinol Metab 2001;86:5992.
Lawrence CB, Ellacott KL, Luckman SM. PRL-Releasing peptide reduces food intake and may mediate satiety signaling. Endocrinology 2002;143:360-7.
Khatoon N, Prakash J. Nutritional quality of microwave-cooked and pressure-cooked legumes. Int J Food Sci Nutr 2004;55:441-8.