ANTIOXIDANT AND FREE RADICAL SCAVENGING ACTIVITY OF THE MIXTURE OF ETHANOLIC EXTRACTS OF ALPINIA SPECIOSA AND ALPINIA CALCARATA RHIZOME
Keywords:
Antioxidants, Free radicals, Alpinia speciosa, Alpinia calcarata, Total Phenolics, and flavonoids, DPPHAbstract
Objective: The present study was aimed to investigate the antioxidant and free radipotentials of the mixture ethanolic extract of rhizomes of Alpinia speciosa and Alpinia calcarata.
Methods: The ethanolic mixture extract of rhizomes was prepared in the ratio of 50:50 of Alpinia speciosa and Alpinia calcarata was subjected to the analysis of phytochemicals, total phenolics and flavonoid contents and free radical scavenging activities such as DPPH, Nitric oxide radical, Superoxide radical, Hydroxyl radical, Ferric reducing antioxidant power, reducing power and Metal ion-chelating activities were determined.
Results: The rhizome mixture extract showed total antioxidant content, good flavonoid and moderate amounts of phenolics content. In DPPH, Nitric oxide and Superoxide radical scavenging activity, IC50 was found 136.22±3.44 µg/ml, 53.67±0.47µg/ml, 74.20±1.48µg/ml respectively with the standard ascorbic acid [3.61±0.20 µg/ml, 49.06±0.18 µg/ml and 39.81±0.22 µg/ml]. For the Hydroxyl radical scavenging activities, the IC50 values were 42.02±2.51 µg/ml with the standard Rutin [17.15±0.02 µg/ml]. The Ferric reducing antioxidant power was found 497.32±7.49 mmol/Fe (II)g with the standard Ascorbic acid 1956.20±74.08 mmol/Fe (II)g. The Metal chelating activity was found 96.23±0.39 mg EDTA/g sample with the standard ascorbic acid 205.86±0.53 mg EDTA/g sample. The reducing power was determined, the concentrations 20 µg/ml absorbance of the rhizome mixture was 0.05 and 100 µg/ml absorbance 0.14 with the standard ascorbic acid was absorbance 0.54 and 2.0.
Conclusion: The results of present study showed that mixture ethanolic extract of rhizomes of Alpinia speciosa and Alpinia calcarata possesses high potential antioxidant activity and could be a potential source a natural antioxidant that could have great importance as therapeutic agents in preventing oxidative stress-related degenerative diseases.
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