IN VITRO BIOMOLECULAR PROTECTIVE EFFECT OF CINNAMOMUM ZEYLANICUM BARK EXTRACTS AGAINST OXIDATIVE DAMAGE
Keywords:
Lipid and DNA models, Lipid peroxidation, DNA damage, antioxidant activity, C zeylanicum barkAbstract
Objective: Free radicals play an important role in the pathogenesis of many disorders by damaging important biomolecules such as lipids, proteins and DNA. Natural antioxidants are safer, cheaper and potential therapeutics to scavenge free radicals. Hence, this study was aimed to assess the biomolecular protective effect of Cinnamomum zeylanicum bark, a culinary spice, on the oxidant-treated membrane lipids and DNA in cell-free systems.
Methods: The total phenolics and flavonoid content of the bark were estimated. The extent of inhibition of lipid peroxidation (LPO) by aqueous, methanolic and chloroform extracts of bark was studied in vitro in three different membrane models such as goat RBC ghosts, goat liver homogenate, and goat liver slices which differ in architecture and lipid composition. The extent of inhibition of oxidant-induced DNA damage by the bark extracts was assessed in commercial DNA preparations such as pBR322, herring sperm, and calf thymus DNA.
Results: The total phenolic content was 153.33±23.09 mg of pyrocatechol equivalents/g, and flavonoid was 33.66±1.15 mg of catechin equivalents/g of powdered bark. All the three extracts exhibited a considerable inhibition of LPO in all the membrane systems, and most significant inhibition was exerted by methanolic extract on the RBC ghosts. All the three extracts were able to revert the oxidant-induced DNA damage, and more significant DNA protection was rendered by methanolic extract on calf thymus DNA.
Conclusion: The present study showed that C. zeylanicum bark is a nutraceutical rich in phenolic antioxidants that can protect biomolecules against oxidative stress.
Keywords: Lipid and DNA models, Lipid peroxidation (LPO), DNA damage, Antioxidant activity, C. zeylanicum bark
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