SIMULTANEOUS DETERMINATION OF SULFAMETHOXAZOLE AND TRIMETHOPRIM IN PHARMACEUTICAL FORMULATIONS BY SQUARE WAVE VOLTAMMETRY
Keywords:
Sulfamethoxazole, Trimethoprim, Glassy carbon electrode, Square wave voltammetry, Pharmaceutical formulationsAbstract
Objective: To develop a simple, fast and low-cost square wave voltammetric (SWV) method for simultaneous determination of sulfamethoxazole (SMX) and trimethoprim (TMP) using a glassy carbon electrode (GCE).
Methods: The SWV parameters were optimized by 2Results: Two well defined oxidation peaks were obtained at 0.96V (SMX) and 1.12V (TMP) in Britton–Robinson buffer (pH 6.0). Under optimized conditions, SWV measurements showed excellent linear response, ranging from 5.5x10-5 to 3.95x10-4 mol L-1 (R=0.9971) and 1.05x10-5 to 1.04x10-4 mol L-1 (R=0.9974) for SMX and TMP, respectively. The detection limits were found to be 8.52x10-6 mol L-1 for SMX and 9.31x10-7 mol L-1 for TMP.
Conclusion: The proposed method was successfully applied to the determination of these antibiotics in commercial pharmaceutical formulations (tablets, oral suspension and injection), without any sample pretreatment. The obtained results are in good agreement with that obtained by the standard HPLC method at a 95% confidence level.Â
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