CHEMICAL COMPOSITION, ANTIOXIDANT, ANTIBACTERIAL AND CYTOTOXICITY ANALYSIS OF BLUMEA LACERA (BURM. F.) DC
Keywords:
Antibacterial, Antioxidant, B lacera, Chemical composition, CytotoxicityAbstract
Objective: To investigate the in vitro antibacterial and antioxidant potential of B. lacera (leaves). The most active extracts were examined for their chemical composition and cytotoxicity.
Methods: The antibacterial activity of five different extracts were examined against 8 bacterial strains (5 Gram-positive and 3 Gram-negative) using resazurin based microtitre dilution assay (RMDA) and disk diffusion assay (DDA). The antioxidant activity of extracts was demonstrated by using DPPH (1, 1-diphenyl-2-picrylhydrazyl) assay and superoxide radical scavenging assay. Chemical composition and cytotoxicity were assessed by using gas chromatography-mass spectrometry (GC-MS) and haemolytic assay, respectively.
Results: According to RMDA, the petroleum ether extract (PEE) and chloroform extract (CE) exhibited highest antibacterial activity. The PEE showed highest activity against Salmonella enterica ser. typhi and Serratia marcescens with MIC i.e. 390.62 μg/ml. Similarly, the CE showed highest antibacterial activity against Bacillus cereus and Micrococcus luteus with MIC i.e. 390.62 μg/ml. In DPPH assay, CE showed the highest radical scavenging activity with IC50 57.46 µg/ml. In GCMS analysis, the principal compounds in PEE and CE were stigmasterol (12.86 %) and L-(+)-ascorbic acid 2, 6-dihexadecanoate (11.73 %), respectively. In haemolytic assay, the PEE and CE showed non-toxic behaviour up to 125 µg/ml and 500 µg/ml, respectively.
Conclusion: The present investigation represents B. lacera as an incredible herb. The PEE and CE were found to possess promising antibacterial and antioxidant properties. The CE exhibited lesser toxicity as compared with PEE.
Downloads
References
Overbye KM, Barrett JF. Antibiotics: where did we go wrong? Drug Discov Today 2005;10:45-52.
Walsh C. Molecular mechanisms that confer antibacterial drug resistance. Nature 2000;406:775-81.
Butler MS, Blaskovich MA, Cooper MA. Antibiotics in the clinical pipeline in 2013. J Antibiot 2013;66:571-91.
Yarney J, Donkor A, Opoku SY, Yarney L, Agyeman I, Abakah AC, et al. Characteristics of users and implications for the use of complementary and alternative medicine in Ghanaian cancer patients undergoing radiotherapy and chemotherapy: a cross-sectional study. BMC Complementary Altern Med 2013;13:1-9.
Gulcin I. Comparison of in vitro antioxidant and antiradical activities of L-tyrosine and L-Dopa. Amino Acids 2007;32:431-8.
Strobel G, Ford E, Worapong J, Harper JK, Arif AM, Grant DM, et al. Isopestacin, a unique isobenzofuranone from Pestalotiopsis microspora possessing antifungal and antioxidant properties. Phytochemistry 2002;60:179-83.
Rahman A, Bhuiyan MA, Shahriar M. Preliminary in vitro biological, phytochemical screenings and in vitro antioxidant activities of Blumea lacera (Burm. f.) DC. Int Arch Appl Sci Technol 2013;4:1-7.
Singh B, Singh S, Singh S, Ali L, Mahajan R. Ethnobotanical significance and antimicrobial activity of Blumea lacera (Roxb.) DC. Int J Pharm Biol Sci Arch 2010;1:314-6.
Jahan K, Kundu SK, Bake MA. Evaluation of antimicrobial and cytotoxic activities of the mehanolic and petroleum ether extract of Blumea lacera Burm. f in Bangladesh. J Pharmacogn Phytochem 2014;2:104-8.
Jain SP, Verma DM, Singh SC, Singh JS, Kumar S. Flora of Haryana. Central institute of medicinal and aromatic plants. Lucknow, India; 2000.
Dhankar S, Dhankar S, Kumar M, Ruhil S, Balhara M, Chhillar AK. Analysis towards innovative herbal antibacterial and antifungal drugs. Recent Patents Anti-Infective Drug Discovery J 2012;7:242-8.
Nisar M, Tariq SA, Marwat IK, Shah MR, Khan IA. Antibacterial, antifungal, insecticidal, cytotoxicity and phytotoxicity studies on Indigofera gerardiana. J Enzyme Inhib Med Chem 2009;24:224-9.
Sharma B, Kumar P. Extraction and pharmacological evaluation of some extracts of Tridax procumbens and Capparis decidua. Int J Appl Res Nat Prod 2009;1:5-12.
Harborne JB. Phytochemical methods. 2nd ed. Chapman and Hall: London; 1991.
Rajeswara RP, Sambasiva RE, Yasodhara B, Dasari VSP, Mallikarjuna RT. In vitro antioxidant and antibacterial activities of different fractions of Heliotropium indicum L. J Pharm Res 2012;5:1051-3.
Sharma B, Jain P, Barkha, Dabur R. Antioxidant and antibacterial activity of Digera muricata (L.) Mart. Int J Innovative Biol Res 2014;1:181-8.
Sermakkani M, Thangapandian V. GC-MS analysis of Cassia italica leaf methanol extract. Asian J Pharm Clin Res 2012;5:90-4.
Chhillar AK, Arya P, Mukherjee C, Gupta P, Yadav Y, Yadav V, et al. Microwave-assisted synthesis of antimicrobial dihydropyridines and tetrahydropyrimidin-2-ones: Novel compounds against Aspergillosis. Bioorg Med Chem 2006;14:973-81.
Ruhil S, Kumar V, Balhara M, Malik M, Dhankhar S, Kumar M, et al. In vitro evaluation of combination of polyenes with EDTA against Aspergillus spp. by different methods (FICI and CI, Model). J Appl Microbiol 2014;117:643-53.
Khair A, Ibrahim M, Ahsan Q, Homa Z, Kuddus MR, Rashid RB, et al. Pharmacological activities of Blumea lacera (Burm. f) DC: a medicinal plant of Bangladesh. Br J Pharm Res 2014;4:1677-87.
Hac LV, Muoi TT. Essential oils of Blumea lacera (Burm. F) DC. (Asteraceae) produced from aerial parts of plants grown in central of Vietnam. J Essent Oil Bear Plants 2003;6:36-40.
Ragasa CY, Wong J, Rideout JA. Monoterpene glycoside and flavonoids from Blumea lacera. J Nat Med 2007;61:474-5.
Haque MA, Kamal ATM, Chowdhury KAA, Chowdury MIA, Hassan MF, Anaytulla. Phytochemical investigation and assessment of in vivo and in vitro pharmacological activities of Blumea lacera (Burm. F.) DC. World J Pharm Res 2015;4:120-30.
Uddin SJ, Grice D, Tiralongo E. Cytotoxic effects of Bangladeshi medicinal plant extracts. J Evidence-Based Complementary Altern Med 2011:1-7. Doi.org/10.1093/ecam/nep111.