IN VITRO ASSESSMENT OF PRASARANI SANDHAN, A TRADITIONAL POLYHERBAL AYURVEDIC MEDICINE, FOR IMMUNOSTIMULATING ACTIVITY IN SPLENIC CELLS OF BALB/C MICE
Keywords:
Prasarani Sandhan, Ayurvedic medicine, Immunostimulant, Splenocytes, Immunoglobulin M, Differentiation, Proliferation, BALBc miceAbstract
Objective: The objective of the present study was to evaluate the immunostimulating potential of an Ayurvedic preparation Prasarani Sandhan (PRS) by measuring immunoglobulinM (IgM) production and splenocytes proliferation in vitro.
Methods: Freshly prepared BALB/c mice splenocytes were treated with 0.25, 0.5, 0.75, 1, 1.5, 2, 3, and 4% (v/v) of PRS and the cells were sub-cultured at 37°C, humidified atmosphere containing 5% CO2 for 120 hours. IgM production and cells proliferation were determined by enzyme-linked immunosorbent assay (ELISA) and 3-(4,5-dimethylthiazol-2-y)-2,5-diphenylterazolium bromide (MTT) methods, respectively. The presence of endotoxin was determined by treating PRS with polymyxin B in culture.
Results: PRS at the doses of 0.25, 0.5, 0.75, 1, 1.5% (v/v) significantly promoted polyclonal IgM productions (1.847, 2.024, 1.986, 1.958, and 1.465 µg/mL, respectively) compared to control (0.358 µg/mL). Similarly, 0.25, 0.5, 0.75, and 1% (v/v) of the preparation significantly stimulated splenocytes proliferation (absorbance 0.346, 0.394, 0.385, and 0.378 respectively, at 570 nm) compared to control (Abs. 0.156). The highest increment of IgM production and splenocytes proliferation by 0.50% PRS was 5.65 and 2.52-times than control. Polymixin B treated PRS significantly reduced its ability for IgM production and cells proliferation by 51.5% and 30%, respectively. Thus, the findings suggest that the apparently observed enhancement of IgM by PRS was due to the contamination of bacterial endotoxin, PRS has no immunostimulating property.
Conclusion: This is the first report on the immunostimulating activity of PRS. Prasarani Sandhan was contaminated with bacterial endotoxin and the preparation failed to exhibit immunostimulating activity in vitro.
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References
Sarker MMR. Adulteration of herbal medicines and dietary supplements with undeclared synthetic drugs: dangerous for human health. Int J Pharm Pharm Sci 2014;6:1-2.
Imam H, Mahbub NU, Khan MF, Hana HK, Sarker MMR. Alpha amylase enzyme inhibitory and anti-inflammatory effect of Lawsonia inermis. Pak J Biol Sci 2013;16:1796-1800.
Sarker MMR, Choudhuri MSK, Zhong M. Effect of Chandanasav, an Ayurvedic formulation, on mice whole splenocytes for the production of IgM and proliferation of cells: a preliminary study. Int J Pharm Sci Res 2012;3:1294-9.
Chakraborty S, Rajia S, Choudhuri MSK, Hossain MF, Sattar M, Shrestha T. Some pharmacological findings of non-therapeutic importance of an Ayurvedic preparation Chandanasava. Orient Pharm Exp Med 2006;6:157-60.
Dewalkar LP, Shambharkar RB, Masram SC. Immunostimulatory effects of etiolated wheat grass, Triticum aestivum L. on dexamethasone induced immunosuppressed albino rats. Int J Pharm Pharm Sci 2014;6:556-8.
Anonymous. Bangladesh National Formulary of Ayurvedic Medicine, National Unani and Ayurvedic Formulary Committee Bangladesh, Board of Unani and Ayurvedic System of Medicine, Bangabandhu Avenue, Dhaka, Bangladesh; 2011.
http: //www.kundeswari.com/ashava.htm [Last accessed on 2014 May 13]
http: //jatmed.net/index.php/current/198-genarel-article56 [Last accessed on 2014 May 13]
http: //jatmed.net/index.php/current/197-genarel-article55 [Last accessed on 2014 May 13]
Samad MB, Kabir A-U, D’Costa NM, Hannan JMA, Prasarani Sandhan. A polyherbal preparation shows anti-nociceptive and anti-inflammatory activities. British J Pharm Res 2013;3:232-46.
Khan S, Balick MJ. Therapeutic plants of Ayurveda: a review of selected clinical and other studies for 166 species. J Altern Complement Med 2001;7:405-515.
Patwardhan B, Vaidya ADB, Chorghade M. Ayurveda and natural products drug discovery. Curr Sci 2004;86:789-99.
Sarker MMR, Nahar S, Shahriar M, Seraj S, Choudhuri MSK. Preliminary study of the immunostimulating activity of an ayurvedic preparation, Kanakasava, on the splenic cells of BALB/c mice in vitro. Pharm Biol 2012;50:1467-72.
Sarker MMR, Nimmi I, Kawsar MH. Preliminary screening of six popular fruits of bangladesh for in vitro igm production and proliferation of splenocytes. Bangladesh Pharm J 2012;15:31-7.
Sarker MMR, Mazumder MEH, Rashid MHO. In Vitro enhancement of polyclonal igm production by ethanolic extract of nigella sativa l. seeds in whole spleen cells of female BALB/c mice. Bangladesh Pharm J 2011;14:73-7.
Hansen MB, Neilsen SE, Berg K. Re-examination and further development of a precise and rapid dye method for measuring cell growth/cell kill. J Immunol Methods 1989;119:203-10.
Sarker MMR, Gohda E. Promotion of anti-keyhole limpet hemocyanin IgM and IgG antibody productions in vitro by red bell pepper extract. J Func Foods 2013;5:1918-26.
Sarker MMR, Zhong M. Keyhole Limpet Hemocyanin augmented the killing activity, cytokine production and proliferation of NK cells, and inhibited the proliferation of Meth A carcoma cells in vitro. Indian J Pharmacol 2014;46:28-33.
Sarker MMR. Induction of humoral immunity through the enhancement of IgM production in murine splenic cells by ethanolic extract of seed of Piper nigrum L. J Sci Res 2012;4:751-6.
Goto T, Sarker MMR, Zhong M, Tanaka S, Gohda E. Enhancement of immunoglobulin M Production in B cells by the Extract of Red Bell Pepper. J Health Sci 2010;56:304-9.
Morrison DC, Jacobs DM. Binding of polymyxin B to the lipid A portion of bacterial lipopolysaccharides. Immunochem 1976;13:813-8.