FORMULATION AND EVALUATION OF CARBOPOL 940 BASED GLIBENCLAMIDE TRANSDERMAL GEL
Keywords:
Glibenclamide, Nil, Propylene glycol, Oleic acid, Skin permeability, Transdermal gelAbstract
Objective: To investigate the effectiveness of combination of permeation enhancers on the transdermal delivery of Glibenclamide.
Methods: The formulations were subjected to various physiochemical studies and in vitro permeation studies. The influence of β – cyclodextrin on the in vitro percutaneous absorption of Glibenclamide (GBM) and its combined effect with propylene glycol (PG) and oleic acid (OA) was studied using Franz-type diffusion cell using a cellophane membrane. The receiver solution was phosphate buffer (pH 7.4). The permeability study was carried out for 12 hours. To increase the aqueous solubility of GBM, it was incorporated as its inclusion complex with β – cyclodextrin. The inclusion complex was thoroughly characterized using techniques, including differential scanning calorimetry and scanning electron microscopy.
Results: The combination of these penetration enhancers synergistically enhanced percutaneous penetration of Glibenclamide gel. During release, data followed the korsmeyer peppas kinetic. It was found that highest flux was obtained by formulation containing 1:2 w/w β – cyclodextrin, 25% propylene glycol and 10% oleic acid during in vitro permeation studies.
Conclusion: Transdermal gel formulation containing Glibenclamide can be prepared by direct dispersion method, using Carbopol 940 as a carrier. Combination of penetration enhancers profoundly increases the drug flux to the desired extent. Comparing in vitro drug release data, formulation F10 showed better release pattern and physiochemical characteristics and no skin irritation reactions.
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