CHEMICAL COMPOSITION AND HEPATOPROTECTIVE EFFECTS OF POLYPHENOLIC FRACTION FROM RIVEA HYPOCRATERIFORMIS IN PARACETAMOL INDUCED LIVER DAMAGE IN WISTAR ALBINO RATS
DOI:
https://doi.org/10.22159/ijpps.2016v8i10.13606Keywords:
Antioxidant, Hepatoprotective, Rivea hypocrateriformis, HPLC, PhenolicAbstract
Objective: This study was designed to chemical composition and hepatoprotective effects of a polyphenolic fraction of aerial parts of R. hypocrateriformis (PPFRH). It was shown to exhibit strong in vitro lipid peroxidation and scavenging activity against hydroxyl radical.
Methods: The chemical composition of a polyphenolic fraction of R. hypocrateriformis was analyzed by High-performance liquid chromatography method. Hepatocellular injuries induced by paracetamol were assessed by liver damage in Wistar albino rat; the hepatoprotective effect was evaluated by biochemical parameters in rat serum, antioxidant hydroxyl radical scavenging activity and lipid peroxidation in liver tissue.
Results: The polyphenolic fraction of aerial parts of R. hypocrateriformis for lipid peroxidation is significantly (p<0.05). In the hepatoprotective activity of liver enzymes and hepatic necrosis were significantly (p<0.001) closer to paracetamol. The correlation coefficient between the hydroxyl scavenging radical and total phenolic and flavonoid contents were found to be R² = 0.9045 and R² = 0.8876 suggesting the contribution of phenolic and flavonoid compounds of the polyphenolic fraction of aerial parts of R. hypocrateriformis by 90% and 88% to its radical scavenging activity.
Conclusion: The polyphenolic fraction of aerial parts of R. hypocrateriformis possesses a significant protective effect against acute hepatotoxicity induced by paracetamol and which may be due to the phenolic and flavonoid components.
Downloads
References
Yan F, Zhang QY, Jiao L, Han T, Zhang H, Qin LP. Synergistic hepatoprotective effect of Schisandrae lignans with Astragalus polysaccharides on chronic liver injury in rats. Phytomedicine 2009;16:805-13.
Yang JY, Li Y, Wang F, Wu CF. Hepatoprotective effects of apple polyphenols on CCl4-induced acute liver damage in mice. J Agric Food Chem 2010;58:6525-31.
Muriel P, Rivera-Espinoza Y. Beneficial drugs for liver diseases. J Appl Toxicol 2008;28:93-103.
Samojlik I, Lakic N, Mimica-Dukic N, Dakovic-Svajcer K, Bozin B. Antioxidant and hepatoprotective potential of essential oils of coriander (Coriandrum sativum L.) and caraway (Carum carvi L.) (Apiaceae). J Agric Food Chem 2010;58:8848-53.
Shimoda H, Tanaka J, Kikuchi M, Fukuda T, Ito H, Hatano T, et al. Walnut polyphenols prevent liver damage induced by carbon tetrachloride and D-galactosamine: hepatoprotective hydrolyzable tannins in the kernel pellicles of walnut. J Agric Food Chem 2008;56:4444-9.
Bhoopat L, Srichairatanakool S, Kanjanapothi D, Taesotikul T, Thananchai H, Bhoopat T. Hepatoprotective effects of lychee (Litchi chinensis Sonn.): a combination of antioxidant and antiapoptotic activities. J Ethnopharmacol 2011;36:55-66.
Halliwell B, Gutteridge JMC, Cross CE. Free radicals, antioxidants, and human disease: where are we now? J Lab Clin Med 1992;119:598-620.
Girish C, Koner BC, Jayanthi S, Ramachandra Rao K, Rajesh B. Hepatoprotective activity of picroliv, curcumin and ellagic acid compared to silymarin on paracetamol induced liver toxicity in mice. Fundam Clin Pharmacol 2009;23:735-45.
Franchesca CM, Gutierrez M, Leano DR, Solidum JN. Evaluation of the hepatoprotective activity of Citrus microcarpa Bunge (Family Rutaceae) fruit peel against acetaminophen-induced liver damage in male BFAD-Sprague Dawley rats. Int J Chem Environ Eng 2010;1:127-32.
Nirmala M, Girija K, Lakshman K, Divya T. Hepatoprotective activity of Musa paradisiaca on experimental animal models. Asian Pac J Trop Biomed 2012;2:11-5.
Manoj S, Mohanty PK, Jaliwala YA. Hepatoprotective activity of fruits of Prunus domestica. Int J Pharma Bio Sci 2011;2:439-53.
Austin FD, Ghazanfar S. Convolvulaceae-Flora of Pakistan, edited by E Nasir, SI Ali. Islamabad: Pan Graphics; 1979;126:1–64.
Kirtikar KR, Basu BD. Indian Medicinal plants. 1st edition New Delhi: International Book publisher and Distributors; 1935.
Dhavan BN, Dubey MP, Mehrotra BN. Screening of Indian plants for biological activity–IX. Indian J Exp Biol 1980;18:594-606.
Shivalingappa H, Biradar JS, Srudresh K. Antiimplantation activity of alcoholic extract of Rivea hypocrateriformis. Indian J Pharm Sci 1999;61:1309-10.
Rajyalakshmi P, Venkatalakshmi K, Venkatalakshmamma Y, Jyothsna K, Balachandramanidevi N, Suneetha V. Total carotenoid and β-carotene contents of forest green leafy vegetables consumed by tribals of south India. Plant Foods Hum Nutr 2001;56:225-38.
Rajyalakshmi P, Venkatalakshmi TVN, Padmavathi P, Suneetha V. Effect of processing on β-carotene content in forest green leafy vegetables consumed by tribals of south India. Plant Food Hum Nutr 2003;58:1-10.
Zamarrud V, Uddin A, Shazia A, Faryal Wali M, Qaiserc M. Desmethyl bergenin hemihydrate. Acta Crystallogr 2006;62:4626-8.
Cicco N, Lanorte M, Paraggio M, Viggiano M, Lattanzio V. A reproducible, rapid and inexpensive folin-ciocalteu micro-method in determining phenolics of plant methanol extracts. Microchem J 2009;91:107-10.
Godipurge SS, Rahber S, Biradar JS, Mahurkar N. Evaluation of pharmacological activities of Rivea hypocrateriformis in experimental animal models. Int J Toxicol Pharmacol Res 2015;7:65-73.
Harbone JB. Phytochemical methods, a guide to modern technique of plant analysis. New York: Chapman and Hall; 2007. p. 4-8.
Singleton VL, Orthofer R, Lamuela-Raventos RM. Analysis of total phenols and other oxidation substrates and antioxidants by means of folin-ciocalteu reagent. In oxidants and antioxidants, Part A; Packer L. Ed. Elsevier Academic Press: San Diego, CA, USA; 1999;299:152-78.
Jia ZH, Tang MC, Wu JM. The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals. Food Chem 1999;64:555-9.
Kursad Yapara, Asim K, Mahmut K, Onur A, Recai T, Serpil E. Hepatoprotective effect of L-carnitine against acute acetaminophen toxicity in mice. Exp Toxicol Pathol 2007;59:121-8.
Takate SB, Pokharkar RD, Chopade VV, Gite VN. Hepatoprotective activity of the ethyl acetate extract of Launaea intybacea (jacq) beauv in paracetamol induced hepatotoxicity in albino rats. Int J Pharm Sci Rev Res 2010;1:72-4.
Sabir SM, Rocha JBT. Water-extractable phytochemicals from Phyllanthus niruri exhibit distinct in vitro antioxidant and in vivo hepatoprotective activity against paracetamol-induced liver damage in mice. Food Chem 2008;111:845-51.
Parmar SR, Vashrambhai PH, Kalia K. Hepatoprotective activity of some plants extract against paracetamol induced hepatotoxicity in rats. J Herbal Med Toxicol 2010;4:101-6.
Reitman S, Frankel AS. A colorimetric method for the determination of serum glutamic oxalacetic and glutamic pyruvic transaminases. Am J Clin Pathol 1957;28:56-63.
Keiding R, Horder M, Gerhardt Denmark W, Pitkanen E, Tenhunen R, Stromme JH. The committee on enzymes of the Scandinavian society for clinical chemistry and clinical physiology: recommended methods for the determination of four enzymes in the blood. Scand J Clin Lab Invest 1974;33:291-306.
Jiang J, Rushlow CA, Zhou Q, Small S, Levine M. Individual dorsal morphogen binding sites mediate activation and repression in the Drosophila embryo. EMBO J 1992;11:3147-54.
Nagai T, Myoda T, Nagashima T. Antioxidative activities of water extract and ethanol extract from field horsetail (tsukushi) Equisetum arvense L. Food Chem 2005;91:389-94.
Landete JM. Dietary intake of natural antioxidants: vitamins and polyphenols. Crit Rev Food Sci Nutr 2013;53:706-21.
Bors W, Michel C, Stettmaier K. Structure–activity relationships governing antioxidant capacities of plant polyphenols. Methods Enzymol 2001;335:166-80.
Maillard MN, Souma MH, Boivinb P, Berset C. Antioxidant activity of barley and malt: relationship with phenolic content. LWT-Food Sci Technol 1996;3:238-44.
Liu RH. Health benefits of fruits and vegetables are from additive and synergistic combination of phytochemicals. Am J Clin Nutr 2003;78:517S-20S.
Wagner C, Fachinetto R, Corte CLD, Brito VB, Severo D, Dias G, et al. Quercitrin, a glycoside form of quercetin, prevents lipid peroxidation in vitro. Brain Res 2006;1107:192-8.
Jeoung D, Kim JH, Lee YH, Baek M, Lee S, Baek MI, et al. DNA microarray analysis of the transcriptional response to hyper in in human gastric cancer cells. J Microbiol Biotechnol 2002;12:664-8.
Kebieche M, Lakroun Z, Lahouel M, Bouayed J, Meraihi Z, Soulimani R. Evaluation of epirubicin-induced acute oxidative stress toxicity in rat liver cells and mitochondria, and the prevention of toxicity through quercetin administration. Exp Toxicol Pathol 2009;61:161-7.
Drotman RB, Lawhorn GT. Serum enzymes as indicators of chemically induced liver damage. Drug Chem Toxicol 1978;1:163-71.
Akachi T, Shiina Y, Ohishi Y, Kawaguchi T, Kawagishi H, Morita T. Hepatoprotective effects of flavonoids from she was a (Citrus depressa) against D-galactosamine-induced liver injury in rats. J Nutr Sci Vitaminol (Tokyo) 2010;56:60-7.
Mondal A, Maity TK, Pal D, Sannigrahi S, Singh J. Isolation and in vivo hepatoprotective activity of Melothria heterophylla (Lour.) Cogn. against chemically induced liver injuries in rats. Asian Pac J Trop Med 2011;4:619-23.
Murugesan G, Sundaram R, Samuel JI. Hepatoprotective and antioxidant properties of marine halophyte Luminetzera racemosa bark extract in CCL4 induced hepatotoxicity. Asian Pac J Trop Med 2011;4:462-5.
Mbaoji FN, Ezike AC, Nworu CS, One to CA, Nwabunike IA, Okoli IC, et al. Antioxidant and hepatoprotective potentials of stemonocoleus micranthus harms (fabaceae) stem bark extract. Int J Pharm Pharm Sci 2016;8:47-51.
Madhukiran P, Ganga Rao B. In vitro evaluation for free radical scavenging activity of methanolic leaf extract of Cyathea gigantean (wall. Ex hook.). Int J Pharm Res Dev 2011;3:2.
Siddappa K, Hanamshetty PC, Mane SB, Nagabhushana MM. Development and validation of spectrophotometric method for the determination of cyclophosphamide in bulk drug and its pharmaceutical dosage form. Int J Pharm Pharm Sci 2013;5:597-600.
Kubow S. Toxicity of dietary lipid peroxidation products. Trends Food Sci Technol 1990;1:67-71.
Jadhav SJ, Nimbalkar Kulkarni AD, Madhavi DL, Deshpande SS, Salunkhe DK. Lipid oxidation in biological and food systems. In: Food antioxidants Eds Dekker: New York; 1996. p. 5-63.
Halliwell B, Guttridge JMC. In: Free Radicals in Biology and Medicine. 2nd ed. Japan Scientific Societies Press: Tokyo, Japan; 1989.
Hochestein P, Atallah AS. The nature of oxidant and antioxidant systems in the inhibition of mutation and cancer. Mutat Res 1988;202:363-75.
Gordon MF. The mechanism of antioxidant action in vitro. In Food antioxidants Hudson BJF Ed Elsevier Applied Science London UK; 1990. p. 1-18.
Burits M, Bucar F. Antioxidant activity of nigella sativa essential oil. Phytother Res 2000;14:323-8.
Published
How to Cite
Issue
Section
