EVALUATION OF THE PROTECTIVE EFFECT OF ETHANOLIC EXTRACT OF LEAVES OF PUNICA GRANATUM LINN. ON FORCED SWIMMING INDUCED CHRONIC FATIGUE SYNDROME IN MICE
Keywords:Punica granatum Linn, Chronic fatigue, Forced swimming, Imipramine
Objective: To study the protective effect of ethanolic extract of leaves of Punica granatum Linn. (EEPG) on forced swimming induced chronic fatigue syndrome (CFS) in mice.
Methods: Male albino mice of 25-40 grams were grouped into five groups taking 5 mice in each. Group A served as naÃ¯ve control, Group B as stress control, Group C and D received EEPG at a dose of 100 mg/kg and 200 mg/kg respectively. Group E was given a standard drug (Imipramine 20 mg/kg). All animals received their respective agent orally daily for 7 d. Except for group A animals, animals in all other groups were subjected to force swimming 6 min daily for 7 d to induce a state of chronic fatigue. Animals were assessed for the duration of immobility on day 1st, 3rd, 5th and 7th. Level of anxiety (elevated plus maze and mirrored chamber test) and locomotor activity (open field test) were assessed 24 h after last force swimming which was followed by estimation of oxidative biomarkers in brain homogenate.
Results: Treatment with EEPG (100 mg/kg and 200 mg/kg) and imipramine resulted in a statistically significant (pâ‰¤0.05) reduction in anxiety and duration of immobility and there was a significant increase in locomotor activity when compared to stress control group. Significant reduction in MDA level and increase in catalase level were seen in EEPG and imipramine-treated group compared to stress control group.
Conclusion: The study confirmed that EEPG has protective action effect against experimentally induced CFS.
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