STUDIES ON ACUTE TOXICITY OF HEPTOPLUS IN EXPERIMENTAL RATS
DOI:
https://doi.org/10.22159/ijpps.2016v8i11.13934Keywords:
Heptoplus, Phyllanthin, Acute toxicityAbstract
Objective: To evaluate acute toxicity of Heptoplus (polyherbal drug) in Sprague-Dawley rats and to identify the active ingredients of the herbal drug.
Methods: Heptoplus was subjected to preliminary phytochemical screening and the HPTLC fingerprint profile of herbal drug was documented. OECD guideline 423-Acute toxic class method was followed to evaluate the oral toxicity of Heptoplus in Sprague-Dawley rats. In acute toxicity studies, Group I (control) rats received 0.5% of carboxymethyl cellouse (Vehicle). Group II rats received 2000 mg/kg b. w of Heptoplus. The rats were observed on the day of dosing and thereafter for 14 d, for any toxic effect.
Results: Preliminary phytochemical analysis of Heptoplus revealed total phenol, flavonoid, carbohydrate, and tannins as its major constituents. The total phenol and flavonoid content of Heptoplus was found to be 170 μg of gallic acid and 162 μg of quercetin equivalent. HPTLC analysis proved that phyllanthin is an active compound of Heptoplus. Acute oral toxicity assays showed Heptoplus administration did not result in any treatment-related mortality, abnormal clinical signs, and loss of body weight or gross pathological changes in rats. Hence, LD50 value of Heptoplus was found to be greater than 2000 mg/kg b. wt.
Conclusion: Heptoplus contain phyllanthin as an active ingredient. LD50 value of Heptoplus was found to be greater than 2000 mg/kg b. wt.
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References
Abrar HM, Manjusha S, Mohd YM. An acute oral toxicity study of methanolic extract from Tridex procumbens in sprague dawley’s rats as per OECD guidelines 423. Asian J Plant Sci Res 2013;3:16-20.
Lalitha P, Shubashini KS, Jayanthi P. Acute toxicity study of extracts of eichhornia crassipes (mart.) solms. Asian J Pharm Clin Res 2012;5:59-61.
Harborne JB. Phytochemical methods. 2nd ed. Chapman and Hall publications. London: NewYork; 1988. p. 288.
McDonald S, Prenzler PD, Autolovich M, Robards K. Phenolic content and antioxidant activity of olive extracts. Food Chem 2001;73:73-84.
Chang CC, Yang MH, Wen HM, Chern JC. Estimation of total flavonoid content in propolis by two complementary colorimetric methods. J Food Drug Anal 2012;10:178-82.
Karthika K, Jamuna S, Paulsamy S. TLC and HPTLC fingerprint profiles of different bioactive components from the tuber of Solena amplexicaulis. J Pharmacogn Phytochem 2014;3:198-206.
OECD/OCDE 423. OECD guideline for testing of chemicals. Acute oral toxicity-acute toxic class method; 2001.
Humason GL. Animal tissue techniques. 4th ed. San Francisco: WH Freeman Co; 1979.
Anon. ICHS7A: Safety pharmacology studies for human pharmaceuticals. Federal 421 Register 2001;66:36791−2.
Redfern WS, Strang I, Storey S, Heys C, Barnard C, Lawton K, et al. Spectrum of effects detected in the rat functional observational battery following oral administration of non-CNS-targeted compounds. J Pharmacol Toxicol Methods 2005;52:77−82.
Muhi-eldeen Z, Al-Shamma KJ, Al-Hussainy TM, Al-Kaissi EN, Al-Daraji AM, Ibrahim H. Acute toxicological studies on the extract of Iraqi peganum harmala in rats. Eur J Sci Res 2008;4:494-500.
Bilbey DLJ, Salem JH, Grossman MH. The anatomical basis of the straub phenomenon. Br J Pharmacol 1960;15:540–3.
Nath C, Gupta MB, Patnaik GK, Dhawan KN. Morphine-induced straub tail response: mediated by the central µ2-opioid receptor. Eur J Pharmacol 1994;263:203-5.
Zarrindast MR, Alaei-Nia K, Shafizadeh M. On the mechanism of tolerance to morphine-induced straub tail reaction in mice. Pharmacol Biochem Behav 2001;69:419-24.
Herrera-Ruiz M, Gutierrez C, Enrique Jimenez-Ferrer J, Tortoriello J, Miron G, Leon I. Central nervous system depressant activity of an ethyl acetate extract from Ipomoea stans roots. J Ethnopharmacol 2007;112:243–7.
Huang F, Xiong Y, Xu L, Ma S, Dou C. Sedative and hypnotic activities of the ethanol fraction from Fructus Schisandrae in mice and rats. J Ethnopharmacol 2007;110:471–5.
Houghton. The scientific basis for the reputed activity of valerian. J Pharm Pharmacol 1999;51:505-12.
Ravikumar M, kishore DV, Jeyanthi Rebecca. Subchronic toxicity analysis of clerodane using rats. Int J Pharm Pharm Sci 2013;5:286-8.
Saba Shafeen, Srinath Reddy T, Arafath S, Nagarjuna S, Padmanabha Reddy Y. Evaluation of the antianxiety and antidepressant activity of cassia occidentalis leaves. Asian J Pharm Clin Res 2012;5:47-50.
Ittiyavirah SP, Ruby R. Effect of hydro-alcoholic root extract of Plumbago zeylanica l alone and its combination with aqueous leaf extract of Camellia sinensis on haloperidol-induced parkinsonism in Wistar rats. Ann Neurosci 2014;2:47-50.
Derardt R, Journey S, Delevalccee F, Falhout M. Release of prostaglandins E and F in an algogenic reaction and its inhibition. Eur J Pharmacol 1980;61:17-24.
Tajik H, Tamaddonfard E, Hamzeh-Gooshchi N. The effect of curcumin (active substance of turmeric) on the acetic acid-induced visceral nociception in rats. Pak J Biol Sci 2008;11:312-4.
Garner JP, Mason GJ. Evidence for a relationship between cage stereotypies and behavioral disinhibition in laboratory rodents. Behav Brain Res 2002;136:83–92.
Sanberg PR, Bunsey MD, Giordano M, Norman AB. The catalepsy test: its ups and Downs. Behav Neurosci 1988;102:748-59.
Finsterer J. Ptosis: causes, presentation, and management. Chin J Aesthetic Plast Surg 2003;27:193-204.
Iversen PO, Nicolaysen G. Water for life. J Norw Med Assoc 2003;123:3402-5.
