DETERMINATION OF ANTIBACTERIAL, ANTIFUNGAL, BIOACTIVE CONSTITUENTS OF TRIPHALA BY FT-IR AND GC-MS ANALYSIS

Authors

  • V. Eugin Amala Research Scholar, Dept. of Biochemistry, Govt. Arts College (Autonomous), Kumbakonam, Tamilnadu, India
  • M. Jeyaraj Research Scholar, Dept. of Biochemistry, Govt. Arts College (Autonomous), Kumbakonam, Tamilnadu, India

Keywords:

Triphala, Antimicrobial activity, FT-IR and GC-MS

Abstract

Objectives: To characterize the number of phytoconstituents present in triphala using FT-IR and GC-MS.

Methods: Antibacterial activity was measured by disc diffusion method, antifungal activity were analyzed by poisoned food technique, organic analysis was done by FT-IR, phytocomponents were identified by GC-MS analysis.

Results: The major bioactive components were present in methanolic extracts, further screened by GC-MS analysis revealed the presence of 10 bioactive compounds. The results were presented that triphala contains richly 1,2,3-Benzenetriol, 2-Furancarboxaldehyde, 5-(hydroxymethyl)-, 4H-Pyran-4-one, 2,3-dihydro-3,5-dihydroxy-6-methyl-, Furfural, 2H-Pyran-2,6(3H)-dione, D-Allose, n-Hexadecanoic acid, DL-Proline, 5-oxo-, methyl ester, Undecanol-5, 9-Phenanthrenol

Conclusion: Present findings indicated promising antimicrobial and phytocomponents are present and having remarkable number of qualities.

Downloads

Download data is not yet available.

Author Biography

V. Eugin Amala, Research Scholar, Dept. of Biochemistry, Govt. Arts College (Autonomous), Kumbakonam, Tamilnadu, India

V.Eugin Amala

Research Scholar

Department of Biochemistry

Govt. Arts College (Autonomous)

Kumbakonam

Thanjavur District, Pin - 612 001

References

Kulkani P.H. Clinical Assessment of the effect of Sookshma Triphala in Lipoma. J Ayurvedic Res Papers 1995;66-71.

Nadkarni A.K. Indian Materia Medica, Popular Press Ltd, Mumbai, 1976;3rd edn, 1308-15.

Singh P.K. Mycotoxin elaboration in Triphala and its constituents. J Indian Phytopathol 2003;56:380-3.

Reddy TC, Aparoy.P, et al. Kinetics and docking studies of a COX-2 inhibitor isolated from Terminalia bellerica fruits. J Protein Pept Lett 2010;17:1251-7.

Gulati R., Agarwal S. Indian J Exp Biol 1995;33:261-8.

Juss S.S. Triphala-the wonder drug. Indian Med Gaz 1997;131:194-6.

Naik GH, Priyadarshini KI, Hari Mohan. Free radical scavenging reactions and phytochemical analysis of Triphala an ayurvedic formulation. J Curr Sci 2006 90(8):1100-05.

Thakur CP et al. The Ayurvedic Medicines Haritaki, amla and Bahira reduce cholesterol-induced atherosclerosis in rabbits. Int J Cardiol 1988;21:167-75.

Eugin Amala.V, Jeyaraj.M., Biometric, functional group analysis, antibacterial and antifungal activity of Terminalia bellirica Roxb and Phyllanthus emblica L. against some Pathogenic Microorganism. Int J of Sci Res 2014;3(4):531-3.

EuginAmala.V, Jeyaraj.M, Phytochemical, antibacterial and functional group identification of medicinally useful plant Terminalia Chebula Retz., against Staphylococcus aureus, Psuedomonas aeruginosa and Klebsilella pnuemoniae. Indiam J of Ap Res 2014;4(1):23-5.

Agarwal et al. Antibacterial activity of hydro alcoholic extract of Terminalia chebula Retz, on different gram positive and gram negative bacteria. Int J Phar Biol Arch 2010;1(4):458-8.

Kokate C.K, Practical Pharmacognosy, Vallabh Prakashan, Mohammed A., Text Book of Pharmacognosy, CBS Publishers and Distributors 2001;218.16.

Bayer et al. Antibiotic susceptibility testing by standard single disc 1966;13-18.

Schmitz H. Food poisoned technique. J Industrial Engineering Chemical Analyst Education 361-3.

Ogunlesi, M., W. Okiei, E.A.S. Osibote and C. Muotoh. Antimicrobial activity of the essential oil and the fractional samples obtained from the leaves and seeds of Phyllanthus amarus (Euphorbiaceae). Res J Phytochem 2009;3:77-84.

Ganesh,S. and J.J. Vennila,. Phytochemical analysis of Acanthus ilicifolius and Avicennia officinalis by GC-MS. Res J Phytochem 2011;5:60-5.

Published

31-08-2014

How to Cite

Amala, V. E., and M. Jeyaraj. “DETERMINATION OF ANTIBACTERIAL, ANTIFUNGAL, BIOACTIVE CONSTITUENTS OF TRIPHALA BY FT-IR AND GC-MS ANALYSIS”. International Journal of Pharmacy and Pharmaceutical Sciences, vol. 6, no. 8, Aug. 2014, pp. 123-6, https://journals.innovareacademics.in/index.php/ijpps/article/view/1395.

Issue

Section

Original Article(s)