DEVELOPMENT OF IN VITRO METHODOLOGIES FOR INHIBITION OF PATHOGENIC BACTERIA BY POTENTIAL PROBIOTIC LACTOBACILLUS SPS; AN EVIDENCE FOR PRODUCTION OF ANTIMICROBIAL SUBSTANCES

Prabhurajeshwar C.; Kelmani Chandrakanth R.

doi:10.22159/ijpps.2016v8i12.14894

Authors

Prabhurajeshwar C. Medical Biotechnology and Phage Therapy Laboratory, Department of Post Graduate Studies and Research in Biotechnology, Gulbarga University, Gulbarga 585106, Karnataka, India
Kelmani Chandrakanth R. Medical Biotechnology and Phage Therapy Laboratory, Department of Post Graduate Studies and Research in Biotechnology, Gulbarga University, Gulbarga 585106, Karnataka, India

DOI:

https://doi.org/10.22159/ijpps.2016v8i12.14894

Keywords:

Cheese, Antimicrobial activity, Antibiotic susceptibility test, Lactic acid bacteria, Probiotics

Abstract

Objective: Probiotic products consist of specific strains of live bacteria that have potentially favorable health effects. A number of studies provide evidence that milk products with probiotics may be beneficial for digestive health and may improve various digestive problems. The purpose of the present study was to investigate Lactobacillus species with potential activities isolated from different cheese samples of local market.

Methods: A total 42 lactic acid bacteria strains were isolated, fourteen (14/42) best Lactobacillus isolates were selected by preliminary screening as potential probiotics with antimicrobial activity against pathogenic bacteria. All the fourteen Lactobacillus isolates were then characterized in vitro for their probiotic features and antimicrobial activities against pathogens.

Results: The results noticed that all selected Lactobacillus isolates (CH3, CH4 and CH6) were screened and confirmed as Lactobacillus. The isolates were able to grow at different pH, NaCl and bile salts, also exhibited the best antimicrobial activities against pathogens. All the isolates were susceptible to antibiotics used and isolates were also revealed the noticeable aggregation and hydrophobicity studies.

Conclusion: Selected Lactobacillus isolates were considered as ideal, effective probiotic bacteria. Thus, they could be examined further and contribute to preventing and controlling several infections associated with intestine and for human health benefits.

Downloads

Download data is not yet available.

Author Biography

Prabhurajeshwar C., Medical Biotechnology and Phage Therapy Laboratory, Department of Post Graduate Studies and Research in Biotechnology, Gulbarga University, Gulbarga 585106, Karnataka, India

--

References

Aslam S, Qazi JI. Isolation of acidophilic lactic acid bacteria antagonistic to microbial contaminants. Pak J Zool 2010;42:567-73.

Tambekar DH, Bhutada SA, Choudhary SD, Khond MD. Assessment of potential probiotic bacteria isolated from milk of domestic animals. J Appl Biosci 2009;15:815â€“9.

Castillo NA, PerdigÃ³n G, de LeBlanc AdM. Oral administration of a probiotic Lactobacillus modulates cytokine production and TLR expression improving the immune response against Salmonella enterica serovar Typhimurium infection in mice. BMC Microbiol 2011;11:177.

Dixit G, Samarth D, Tale V, Bhadekar R. Comparative studies on potential probiotic characteristics of Lactobacillus acidophilus strains. Eurasia J Biosci 2013;7:1â€“9.

Sumi das P, Bhattacharya MK, Prasad HK, Upadhyaya H, Pal K, Sharma GD, et al. Antimicrobial activity of Lactobacillus fermentum, a volvo vaginal isolate. Asian J Pharm Clin Res 2015;8:371-2.

Gareau MG, Sherman PM, Walker WA. Probiotics and the gut microbiota in intestinal health and disease. Nat Rev Gastroenterol Hepatol 2010;7:503â€“14.

Hemal S, Jayantilal D, Bharat kumar RM. Screening of potentially probiotic Lactobacillus strains isolated from fermented foods, fruits and of human origin. Asian J Pharm Clin Res 2014;7:216-25.

Fuller R. Probiotics in man and animals. J Appl Bacteriol 1989;66:365-78.

Fuller R. Probiotics: the scientific basis Chapman and Hall, London; 1992. p. 398.

Holzapfel WH, Haberer P, Geisen RJ, BjÃ¶rkroth, Schillinger U. Taxonomy and important features of probiotic microorganisms in food nutrition. Am J Clin Nutr 2001;73:365-73

Vernoux JP, Coeuret V, Dubernet S, Bernardeau M, Gueguen M. Isolation, characterization and identification of lactobacilli focusing mainly on cheeses and other dairy products. EDP Sci-Inra 2003;83:269-306.

Oskar A, Meydani SN, Russell RM. Yogurt and gut function. Am J Clin Nutr 2004;80:245-56.

Tannock G. A special fondness for lactobacilli. Appl Environ Microbiol 2004;70:3189-94.

Catanzaro J, Green L. Probiotics in human medicine (Part II). Altern Med Rev 1997;2:296-305.

Walker DK, Gilliland SE. The relationship among bile tolerance, bile salt deconjugation, and Lactobacillus casei, Lactobacillus rhamnosus and assimilation of cholesterol by Lactobacillus acidophilus. J Dairy Sci 1983;76:956-61.

Kilara A. Influence of in vitro gastric digestion on the survival of some lactic cultures. J Dairy Sci 1982;37:129-32.

Petros A, Maragkoundakis PA, Zoumpopoulou G, Miaris C, Kalantzopoulos G, Pot B, et al. Probiotic potential of Lactobacillus strains isolated from dairy products. Int Dairy J 2006;16:189-99.

Pennacchia C, Ercolini D, Blaiotta G, Pepe O, Mauriello G, Villani F, et al. Selection of Lactobacillus strains from fermented sausages for their potential use as probiotics. J Meat Sci Bark 2004;67:309-17.

Giilliland SE, Staley TE, Bush LJ. The importance of bile tolerance of Lactobacillus acidophilus used as a dietary adjunct. J Dairy Sci 1984;67:3045-51.

Bauer AW, Kirby WM, Sherris JC, Turck M. Antibiotic susceptibility testing by standard single disc method. Am J Clin Pathol 1996;45:493â€“6.

National Committee for Clinical Laboratory Standards, author. Tentative Guidelines, M26-TNCCLS: Villanova; 1993.

Ashraf M, Arshad M, Siddique M, Muhammad G. In vitro screening of locally isolated Lactobacillus species for probiotic properties. Pak Vet J 2009;29:186-90.

Toure R, Kheadr E, Lacroix C, Moroni O, Fliss I. Production of antibacterial substances by Bifidobacterial isolates from infant stool active against Listeria monocytogenes. J Appl Microbiol 2003;95:1058â€“69.

GrzeÅ›kowiak L, Collado MC, Salminen S. Evaluation of aggregation abilities between commensal fish bacteria and pathogens. Aquac 2012;356:412â€“6.

Gotcheva V. Assessment of potential probiotic properties of lactic acid bacteria and yeast strains. J Food Biotech 2002;16:211-25.

Osuntoki AA, Ejide OR, Omonigbehin EA. Antagonistic effects on Enteropathogenic and plasmid analysis of Lactobacilli isolated from fermented Dairy products. Biotechnology 2008;7:311-6.

Holt JG, Krieg NR, Sneath PHA, Staley JT, Williams ST. Bergeyâ€™s manual of Determinative Bacteriology: Baltimore; 1994.

Elizete DFRP, Carlos RS. Biochemical characterization and identification of probiotic Lactobacillus for swine. B CEPPA Curitiba 2005;23:299-10.

Burns P, Patrigani F, Serrazanetti D, Vinderola GC, Reinheimer JA, Lanciotti R, et al. Probiotic Crescenza cheese containing Lactobacillus casei and Lactobacillus acidophilus with high-pressure homogenized milk. J Dairy Sci 2008;91:500-12.

Begly M, Gahan CG, Hill C. The interaction between bacteria and bile. FEMS Microbiol Rev 2005;29:625-51.

Makras L, De Vuyst L. The in-vitro inhibition of pathogenic gram-negative bacteria by Bifidobacteria is caused by the production of organic acids. Int Dairy J 2006;16:1049-57.