NEUROPROTECTIVE ACTIVITY OF NOVEL CUR-CA-THIONE AND ITS OXIDATIVE STRESS STUDY
DOI:
https://doi.org/10.22159/ijpps.2016v8i12.15141Keywords:
Nil, Aluminum chloride, Behavioral, CUR-CA-THIONE, NeuroprotectiveAbstract
Objective: Alzheimer's disease a progressive neurodegenerative disorder affected by the formation of amyloid beta and tau proteins. Medicinal plants have been proved significantly for its anti-oxidant and anti-inflammatory activity that might help in treating neurological disorders. Curcumin has been hugely studied in the treatment of various ailments but its water solubility and bioavailability is still a concern but we have tried to exterminate the problem by our formulation CUR-CA-THIONE. Now, we have expanded the study of CUR-CA-THIONE for its neuroprotective estimation by evaluating behavioral, biochemical and histopathological assessment in rats.
Methods: Wistar rats of either sex (M/F: 25-350g; 350-450 g) were selected for study and divided into 8 groups. All animals except NC were given aluminum chloride via oral route throughout the study period (30 d) while group 3-8 received treatment one-hour post aluminum chloride induction from 15th day to 30th day. One week prior to the start of the experiment all animals were given training for behavioral assessment through y-maze and morris water maze with the weekly assessment. On 30th-day post completion of study rat brain was isolated to study biochemical and histopathological examination.
Results: The results of the behavioral assessment in CUR-CA-THIONE complex showed an increase in total arm entries in Y-maze and decrease in time duration for morris water maze test. A significant (p<0.01) decrease in lipid peroxidation, superoxide dismutase, acetylcholine and total protein levels in formulations while significant (p<0.01) increase in glutathione and catalase level was observed.
Conclusion: The given formulation shows that curcumin-casein-glutathione complex shows potential action as a neuroprotective effect.
Â
Downloads
References
Belanger M, Magistretti P. The role of astroglia in neuroprotection. Transl Res 2009;11:281–95.
Gella A, Durany N. Oxidative stress in Alzheimer disease. Cell Adhes Migr 2009;3:88–93.
Zhao Y, Zhao B. Oxidative stress and the pathogenesis of alzheimer’s disease. Oxid Med Cell Longev 2013. http://dx.doi.org/10.1155/2013/316523
Akter K, Lanza E, Martin S, Myronyuk N, Rua M, Raffa R. Diabetes mellitus and Alzheimer’s disease: Shared pathology and treatment?. Br J Clin Pharmacol 2011;71:365–76.
Terry A, Buccafusco J. The cholinergic hypothesis of age and alzheimer’s disease-related cognitive deficits: recent challenges and their implications for novel drug development. J Pharmacol Exp Ther 2003;306:821–7.
Nemat A, Yassin Z, Siham M, Shenawy E, Karam A, Nadia A, et al. Effect of Boswellia serrata on Alzheimer’s disease induced in rats. J Arab Soc Med Res 2013;8:1–11.
Mufson E, Counts S, Perez S, Ginsberg S. Cholinergic system during the progression of Alzheimer’s disease: therapeutic implications. Expert Rev Neurother 2009;8:1703–18.
Hshieh T, Fong T, Marcantonio E, Inouye S. Cholinergic deficiency hypothesis in delirium: a synthesis of current evidence. J Gerontol Ser A 2008;63:764–72.
Daiello L, Festa E, Ott B, Heindel W. Cholinesterase inhibitors improve visual attention in drivers with Alzheimer’s disease. Alzheimer’s Dementia J 2016;4:T498.
Zhu X, Perry G, Moreira P, Aliev G, Cash A, Hirai K, et al. Mitochondrial abnormalities and oxidative imbalance in Alzheimer disease. J Alzheimers Dis 2006;9:147–53.
Silva C, Herdeiro R, Mathias C, Panek A, Silveira C, Rodrigues V, et al. Evaluation of antioxidant activity of Brazilian plants. Pharmacol Res 2005;52;3:229-33.
Priyadarsini K. Chemical and structural features influencing the biological activity of curcumin. Curr Pharm Des 2013;19:2093–100.
Menon V, Sudheer A, Aggarwal B, Surh Y, Shishodia S. Anti-oxidant and anti-inflammatory properties of curcumin. Springer. Boston, MA; 2007.
Athira G, Jyothi A. Preparation and characterization of curcumin loaded cassava starch nanoparticles with improved cellular absorption. Int J Pharm Pharm Sci 2014;6:171–6.
Van K, Havekes R, Bos T, Eggen B, Zee E. Exercise improves memory acquisition and retrieval in the Y-maze task: relationship with hippocampal neurogenesis. Behav Neurosci 2007;121:324–34.
Vorhees C, Williams M. Forms of learning and memory. Nat Protoc 2006;1:848–58.
Shin M, Kim H, Baek S, Jung W, Park D, Park J, et al. Neuropep-1 ameliorates learning and memory deficits in an Alzheimer’s disease mouse model, increases brain-derived neurotrophic factor expression in the brain, and causes reduction of amyloid beta plaques. Neurobiol Aging 2014;35:990–1001.
Macdonald I, Olusola O, Osaigbovo U. Effects of chronic ethanol administration on body weight, reduced glutathione (GSH), malondialdehyde (MDA) levels and glutathione-s-transferase activity (GST) in rats. New York Sci J 2010;3:39–47.
Nostrandt J. A modified spectrophotometric method appropriate for measuring cholinesterase activity in tissue from carbaryl treated animals. Fundam Appl Toxicol 1993;21:196–203.
Heath R, Packer L. Photoperoxidation in isolated chloroplasts. I. Kinetics and stoichiometry of fatty acid peroxidation. Arch Biochem Biophys 1968;125:189–98.
Misra H, Fridovich I. The role of superoxide anion in the autoxidation of epinephrine and a simple assay for superoxide dismutase. J Biol Chem 1972;247:3170–5.
Luck H. Methods of enzymatic analysis. Academic Press: New York; 1963.
Lowry O, Rosebrough N. Protein measurement with the folin phenol reagent. J Biol Chem 1951;193:265–75.
Jia Q, Deng Y, Qing H. Potential therapeutic strategies for alzheimer’s disease targeting or beyond beta-amyloid: insights from clinical trials. Biomed Res Int 2014:22. http://dx.doi.org/10.1155/2014/837157.
Singh SK, Srivastav S, Yadav AK, Srikrishna S, Perry G. Overview of alzheimer’s disease and some therapeutic approaches targeting ABeta by using several synthetic and herbal compounds. Oxid Med Cell Longev 2016. http://dx.doi.org/10.1155/2016/7361613
Yokel R, Rhineheimer S, Sharma P, Elmore D, McNamara P. Entry, half-life, and desferrioxamine-accelerated clearance of brain aluminum after a single Al exposure. Toxicol Sci 2001;64:77–82.
Exley C. The pro-oxidant activity of aluminum. Free Radical Biol Med 2004;36:380–7.
Yokel R. Brain uptake, retention, and efflux of aluminum and manganese. Environ Health Perspect 2002;110:699–704.
Kumar A, Prakash A, Dogra S. Neuroprotective effect of carvedilol against aluminium induced toxicity: possible behavioral and biochemical alterations in rats. Pharmacol Reports 2011;63:915–23.
Steffi P, Srinivasan M. Curcumin, a potent anticarcinogenic polyphenol-A review. Asian J Pharm Clin Res 2014;7:1–8.
Couette M, Boisse M, Maison P, Brugieres P, Cesaro P, Chevalier X, et al. Long-term persistence of vaccine-derived aluminum hydroxide is associated with chronic cognitive dysfunction. J Inorg Biochem 2009;103:1571–8.
Prakash A, Shur B, Kumar A. Naringin protects memory impairment and mitochondrial oxidative damage against aluminum-induced neurotoxicity in rats. Int J Neurosci 2013;123:636–45.
Pattipati K. Animal models in drug discovery of alzheimer’s disease: a mini review. EC Pharmacol Toxicol 2016;1:60–79.
Amel B, Omar K, Faiza F, Miloud S, Abdelkader A. Behavior and glutamate transaminase changes in rat exposed to lead and treated by wormwood extract. Int J Pharm Pharm Sci 2016;8:2.
Published
How to Cite
Issue
Section
