MULTIVARIATE ANALYSIS BETWEEN THE PHYTOCHEMICAL FEATURES AND ANTIOXIDANT PROPERTIES OF THE STEMS OF BAUHINIA GLABRA JACQ. (FABACEAE)
Keywords:
Bauhinia glabraJacq, Fabaceae, Antioxidant, Methemoglobin, Phytosterols, Phytochemical features, Multivariate analysisAbstract
Objective: To provide information about the phytochemical features of the crude extract (CruE) and fractions of the forageBauhinia glabra Jacq., Fabaceae, and present themultivariate correlation between its metabolites and the antioxidant properties.
Methods: Studies were carried out by extraction with ethanol, sequential partition with hexane, chloroform and ethyl acetate, determination of the total yield of extraction, qualitative and quantitative estimation of phytochemicals.Then, the evaluation of antioxidant properties by four methods: 1,1-diphenyl picrylhydrazyl free radical scavenging assay (DPPH), the reduction power, the evaluation of inhibition of lipid peroxidation (TBARS) and the evaluation of recovery of content of methemoglobin in erythrocytes.
Results: Phytochemical analysis on CruErevealed the presence of chlorophylls, carotenoids, coumarins, phytosterols, anthocyanins, tannins and flavonoids. Quantitative estimation of metabolites on CruE showed high concentration of phytosterols (42.21±2.34) mg/gdw and total phenolic (58.50±1.98) mg/gdw. The best results onthe antioxidant properties on each assay were the chloroform fraction (ChlF) with 13.76±0.23 and methanolic fraction (MetF) with 13.46±0.45 of half-maximal inhibitory concentration (IC50) values (DPPH).The HexF(60.99±1.76%) and ChlF(64.40±1.73%), both based on reduction power of ascorbic acid. The HexF(73.54±2.74%) of inhibition of lipid peroxidation (TBARS), and HexFwith 7.28±0.36% amount of methemoglobin.
Conclusion: Based on multivariate analysis, HexFthat presented the highest concentration of phytosterols, has the highest reduction power and was more effectivefor inhibition of lipid peroxidation and in recoveringmethemoglobincontent. Therefore, the mechanism of these activities seems to be related to the reduction power presented by phytosterols.
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References
Wunderlin RP. Revision of the arborescent Bauhinias (Fabaceae:Caesalpinioideae:Cercideae) Native to middle. J America Annals of the Missouri Botanical Garden 1983;70:95-127.
Willain Filho A, Breviglieri E, Cechinel Filho V, Santos ARS. Antinociceptive effect of the hydroalcoholic extract of Bauhinia splendensstems in mice. J Pharm Pharmacol 1997;49:823-7.
Silva KL, Cechinel Filho V. Plantas do gênero Bauhinia:Composição quÃmica e potencial farmacológico. J QuÃmica Nova 2002;25:449-54.
Apisantiyakoma S, Kittakoop P, Manyuma T, Kirtikarab K, Bremnerc JB, The btaranonth Y..Novel biologically active bibenzyls from Bauhinia saccocalyxP. J Chemistry and Biodiversity 2004;1:1694-701.
Maheswara M, Rao YK, Siddaiah V, Rao CV. Isolation of new chalcone from the leaves of Bauhinia variegate. Asian J of Chemistry 2006;18:419-22.
Yadav S, Bhadoria BK.Two dimeric flavonoids from Bauhinia purpurea. Indian J of Chemistry 2005;44b:2604-07.
Zhao YY, Cui CB, Cai B, Han B, Sun QS. A new phenanthraquinone from the stems of Bauhinia variegateL. J of Asian Natural Products Res 2005;7:835-8.
Batista JS, Arruda FAV, Azevedo AR, Alves AA. Composição quÃmico-bromatológica do feno de Cipó-de-Escada (Bauhinia glabra Jacq.) em cinco estádios de corte. J Revista Brasileira de Zootecnia 1999;28:914-8.
Archile-Contreras AC, Purslow PP. Oxidative stress may affect meat quality by interfering with collagen turnover by muscle fibroblasts. J Food Res Int 2011;44:582-8.
Gladine C, Morand C, Rock E, Gruffat D, Bauchart D, Durand D. The antioxidative effect of plant extracts rich in polyphenols differs between liver and muscle tissues in rats fed n-3 PUFA rich diets. J Animal Feed Science and Technology 2007;139:257-72.
Morán L, Andrés S, Bodas R, Benavides J, Prieto N, Pérez V, et al. Antioxidants included in the diet of fattening lambs:Effects on immune response, stress, welfare and distal gut microbiota. J Animal Feed Science and Technology 2012;173:177-85.
Harborne JB.Phytochemical Methods:A guide to modern technique of plant analysis. 3rd ed. London (UK):Champman and Hall;1998.
Sazada S, Verma A, Rather AA, Jabeen F, Meghvansi MK. Preliminary phytochemical analysis of some important medicinal and aromatic plants. J Advances in Biological Res 2009;3:188-95.
Dere S, Gunes T, Sivaci R. Spectrophotometric determination of chlorophyll-A, B and total carotenoid contents of some Algae Species using different solvents. Turkish J of Botany 1998;22:13-7.
Daksha A, Jaywant P, Bhagyashree C, Subodh P. Estimation of sterols content in edible oil and ghee samples. Electronic J of Environmental Agricultural and Food Chemistry 2010;9:1593-97.
Folin O, Denis W. A colorimetric method for the determination of phenols (and phenol derivatives) in urine. J Biol Chem 1915;22:305-08.
Ribéreau-Gayon P, Glories Y, Maujean A, Dubordieu D. Handbook of Enology. 2nd ed. Chichester (UK):John Wiley & Sons Ltda;2006.
Hossain MA, AL-Raqmi KAS, AL-Mijizy ZH, Weli AM, Al-Riyami Q. Study of total phenol, flavonoids contents and phytochemical screening of various leaves crude extracts of locally grown Thymus vulgaris. Asian Pacific J of Tropical Biomedicine 2013;3:705-10.
MensorLL, MenezerFS, Leitão GG, Reis AS, Santos TC, Coube CS, et al.Screening of brazilian plant extracts for antioxidant activity by the use of DPPH free radical method. J Phytotherapy Res 2001;15:127-30.
Prieto P, Pineda M, Aguilar M. Spectrophotometric quantitation of antioxidant capacity through the formation of a Phosphomolybdenum Complex:Specific application to the determination of vitamin E. J Analytical Biochemistry 1999;269:337-41.
Morais SM, Catunda Junior EA, Silva ARA, Martins Neto JS. Antioxidant activity of essential oils from Northeastern Brazilian Croton species. J QuÃmica Nova 2006;29:907-10.
Naoum, PC, Radispiel, J., Moraes, MS, Dosagem espectrométrica de metaemoglobina sem interferentes quÃmicos ou enzimáticos. J Revista Brasileira de Hematologia e Hemoterapia 2004;26:19-22.
Fejes S, Blzovics A, Lugasi A, Lemberkovics E, Petri G, Kry A. In vitro antioxidant activity of Anthriscus cerefolium L.(Hoffm.) extracts. J Ethnopharmacol 2000;69:259-65.
Yokozawa T, Chen CP, Dong E, Tanaka T, Nonaka GI, Nishioka I. Study on the inhibitory effect of tannins and flavonoids against the 1, 1-diphenyl-2-picrylhydrazyl radicals. J Biochemical Pharmacology 1998;56:213-22.
Bhardwaj R, Yadav A, Sharma R. Phytochemicals and antioxidant activity in Boerhavia diffusa. Int J Pharm Pharm Sci 2014;6:344-8.
Yoshida Y, Nikki E. Antioxidant effects of phytosterol and its components. J Nutr Sci Vitaminol 2003;49:277-80.