A CHEAP METHOD DEVELOPMENT AND VALIDATION FOR THE QUANTITATIVE ESTIMATION OF ACAMPROSATE CALCIUM IN TABLETS USING UV SPECTROPHOTOMETRY

Authors

  • DANDU VIJAY KUMAR Department of Pharmaceutical analysis and Quality Assurance, Vijaya college of pharmacy, Munaganur (village), Hayathnagar (mandal),Hyderabad 501511, India
  • PARUPUGALLA SWETHA Department of Pharmaceutical analysis and Quality Assurance, Vijaya college of pharmacy, Munaganur (village), Hayathnagar (mandal),Hyderabad 501511, India
  • THOTA CHANDRA MOHAN Department of Pharmaceutical analysis and Quality Assurance, Vijaya college of pharmacy, Munaganur (village), Hayathnagar (mandal),Hyderabad 501511, India
  • A. ASHOK KUMAR Department of Pharmaceutical analysis and Quality Assurance, Vijaya college of pharmacy, Munaganur (village), Hayathnagar (mandal),Hyderabad 501511, India

Keywords:

UV, Acamprosate calcium, Method development, Validation

Abstract

Objective: To develop a cheap and a rapid UV spectrophotometric method for the quantitative estimation of Acamprosate calcium (333mg) in tablets and validate as per ICH guidelines.

Methods: The optimized method uses 100% distilled water as a solvent for the estimation of assay of Acamprosate calcium in tablets at a detection wavelength of 208 nm.

Results: The developed method resulted in Acamprosate calcium exhibiting linearity in the range 30-90μg/ml. The precision is exemplified by relative standard deviation of 0.425%. Percentage Mean recovery was found to be in the range of 98â€102, during accuracy studies. The limit of detection (LOD) and limit of quantitiation (LOQ) were found to be 66mg/ml, and 200 mg/ml respectively.

Conclusion: A cheap and a rapid UV spectrophotometric method was developed and validated for the quantitative estimation of Acamprosate calcium in tablets as per ICH guidelines and hence it can be used for the routine analysis in various pharmaceutical industries.

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Author Biographies

DANDU VIJAY KUMAR, Department of Pharmaceutical analysis and Quality Assurance, Vijaya college of pharmacy, Munaganur (village), Hayathnagar (mandal),Hyderabad 501511, India

Department of Pharmaceutical Analysis and Quality assurance

PARUPUGALLA SWETHA, Department of Pharmaceutical analysis and Quality Assurance, Vijaya college of pharmacy, Munaganur (village), Hayathnagar (mandal),Hyderabad 501511, India

Department of Pharmaceutical Analysis and Quality assurance

THOTA CHANDRA MOHAN, Department of Pharmaceutical analysis and Quality Assurance, Vijaya college of pharmacy, Munaganur (village), Hayathnagar (mandal),Hyderabad 501511, India

Department of Pharmaceutical Analysis and Quality assurance

A. ASHOK KUMAR, Department of Pharmaceutical analysis and Quality Assurance, Vijaya college of pharmacy, Munaganur (village), Hayathnagar (mandal),Hyderabad 501511, India

Department of Pharmaceutical Analysis and Quality assurance

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Published

31-08-2014

How to Cite

KUMAR, D. V., P. SWETHA, T. C. MOHAN, and A. A. KUMAR. “A CHEAP METHOD DEVELOPMENT AND VALIDATION FOR THE QUANTITATIVE ESTIMATION OF ACAMPROSATE CALCIUM IN TABLETS USING UV SPECTROPHOTOMETRY”. International Journal of Pharmacy and Pharmaceutical Sciences, vol. 6, no. 8, Aug. 2014, pp. 371-4, https://journals.innovareacademics.in/index.php/ijpps/article/view/1527.

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