• Pinky Sarmah Department of Biochemistry, Assam down town University, Guwahati 781026, India
  • Nako Kobing Department of Biochemistry, Assam down town University, Guwahati 781026, India
  • Jyotchna Gogoi Department of Biochemistry, Assam down town University, Guwahati 781026, India
  • Ananta Madhab Dutta Department of Chemistry, Assam down town University, Guwahati 781026, India



Anti-amylase activity, Anthelmintic activity, Garcinia pedunculata


Objective: The present study aims to investigate in vitro anthelmintic and anti-amylase properties of the ethanolic fruit extract of Garcinia pedunculata.

Methods: For the study, mature fruits of G. pedunculata were collected from local markets during April-May. Fleshy pericarps of fruits were chopped into small pieces, dried and extracted by using a Soxhlet apparatus. Ethanol extract of G. pedunculata was used for evaluation of in vitro anthelmintic and anti-amylase activities. In vitro anthelmintic activity was evaluated in animal models, Pheretima posthuma, an earthworm species. In vitro anti-amylase activity was evaluated by using zymographic, achromic point analysis (Starch-Iodine method) and spectrophotometric method [Di Nitro Salicylic acid (DNS)–Maltose method].

Results: Ethanolic extract of G. pedunculata showed anthelmintic activity at a concentration of 75 mg/ml, paralysis and death timing was reported at 0.62±0.26 min and 1.42±0.07 min, respectively. The reference standard (Albendazole) showed paralysis time: 2.13±0.28 min and death time: 5.12±0.29 min. In the anti-amylase study, a zymographic density analysis of G. pedunculata showed significant variation in band intensity as compared to Starch–Iodine achromic point analysis and DNS–Maltose method. A concentration of 1.5 mg/ml of extract showed inhibition of amylase: 67.65±1.53 % as compared to other concentrations and control sets.

Conclusion: It could be concluded that ethanolic extract of G. pedunculatahas biological properties which could be utilised in medicine by characterising its bioactive components.



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How to Cite

Sarmah, P., N. Kobing, J. Gogoi, and A. M. Dutta. “IN VITRO ANTHELMINTIC AND ANTI-AMYLASE PROPERTIES OF GARCINIA PEDUNCULATA ROXB. ETHANOLIC EXTRACT”. International Journal of Pharmacy and Pharmaceutical Sciences, vol. 9, no. 1, Jan. 2017, pp. 189-91, doi:10.22159/ijpps.2017v9i1.15395.



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