RESVERATROL INCLUSION COMPLEX WITH β-CYCLODEXTRIN (RCD): CHARACTERIZATION AND EVALUATION OF TOXICITY IN WISTAR RATS

Authors

  • V. S. K. Nishihira Laboratory of Nanotechnology, Post Graduate Program in Nanosciences, Franciscan University Center, Santa Maria, 97010-032, Rio Grande do Sul, Brazil
  • N. J. Mezzomo Laboratory of Experimental Neuropsychobiology, Federal University of Santa Maria (UFSM), Santa Maria, 97105-900, Rio Grande do Sul, Brazil
  • M. D. Baldissera Microbiology and Parasitology Department, Health Sciences Center, Federal University of Santa Maria (UFSM), Santa Maria, 97105-900, Rio Grande do Sul, Brazil
  • R. A. Vaucher Post Graduate Program in Biochemistry and Bioprospecting, Center of Chemistry, Pharmaceutical and Food Science, Federal University of Pelotas (UFPel), Pelotas, 96010-900, Rio Grande do Sul, Brazil
  • C. G. Pinto Laboratory of Nanotechnology, Post Graduate Program in Nanosciences, Franciscan University Center, Santa Maria, 97010-032, Rio Grande do Sul, Brazil
  • A. R. Pohl Laboratory of Nanotechnology, Post Graduate Program in Nanosciences, Franciscan University Center, Santa Maria, 97010-032, Rio Grande do Sul, Brazil
  • P. Gomes Laboratory of Nanotechnology, Post Graduate Program in Nanosciences, Franciscan University Center, Santa Maria, 97010-032, Rio Grande do Sul, Brazil
  • L. F. Rodrigues Junior Laboratory of Chemical Engineering, Franciscan University Center, Santa Maria, 97010-032, Rio Grande do Sul, Brazil
  • C. P. Frizzo Nucleus of Chemistry of Heterocycles, Department of Chemistry, Federal University of Santa Maria (UFSM), Santa Maria, 97105-900, Rio Grande do Sul, Brazil
  • C. R. Bender Nucleus of Chemistry of Heterocycles, Department of Chemistry, Federal University of Santa Maria (UFSM), Santa Maria, 97105-900, Rio Grande do Sul, Brazil
  • L. Fantinel Laboratory of Chemical Engineering, Franciscan University Center, Santa Maria, 97010-032, Rio Grande do Sul, Brazil
  • L. S. Fernandes Laboratory of Nanotechnology, Post Graduate Program in Nanosciences, Franciscan University Center, Santa Maria, 97010-032, Rio Grande do Sul, Brazil
  • J. L. Giongo Laboratory of Pharmaceutical Technology, University of High Uruguay Regional Integrated (URI), Santiago, 97700-000, Rio Grande do Sul, Brazil
  • R. P. Raffin Laboratory of Nanotechnology, Post Graduate Program in Nanosciences, Franciscan University Center, Santa Maria, 97010-032, Rio Grande do Sul, Brazil
  • V. C. Rech Laboratory of Nanotechnology, Post Graduate Program in Nanosciences, Franciscan University Center, Santa Maria, 97010-032, Rio Grande do Sul, Brazil

DOI:

https://doi.org/10.22159/ijpps.2017v9i1.15399

Keywords:

Resveratrol, Nil, Nanoscience, Hemogram, Biochemical and blood serum

Abstract

Objective: The aim of this study was to characterise the resveratrol inclusion complex with β-cyclodextrin (RCD) and evaluate their toxicity in wistar rats.

Methods: The RCD were prepared in ultra-turrax. For characterization of the RCD were used: Fourier transform infra-red Spectroscopy, Nuclear Magnetic Resonance (NMR), Differential Scanning Calorimetry (DSC) and X-ray powder diffraction. The RCD and others 4 treatments were performed by the chronic oral administration in 35 rats during 60 ds. After the treatments they were euthanized and the serum blood were collected to analyzed some hemogram and biochemical parameters including aspartyl aminotransferase (AST); alanine aminotransferase (AST); phosphatase alkaline (ALP); total bilirubin (TB); direct bilirubin (DB); total protein (TP); total cholesterol (TC), triacylglycerol (TAG), very low-density lipoprotein (VLDL), high-density lipoprotein (HDL), calcium, iron and phosphate using fully automated biochemistry analyzer.

Results: The characterization results indicated a successful formation of the RCD. All hematological parameters analysed were within the normal values in all the groups. Furthermore, the hemogram and biochemical parameters were significantly (P>0.05) similar to the control group.

Conclusion: The daily oral administration during 60 d of RCD are not harmful on blood parameters of Wistar rats. Thus, RCD can be used safely for treatment of some metabolic diseases.

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Published

01-01-2017

How to Cite

Nishihira, V. S. K., N. J. Mezzomo, M. D. Baldissera, R. A. Vaucher, C. G. Pinto, A. R. Pohl, P. Gomes, L. F. R. Junior, C. P. Frizzo, C. R. Bender, L. Fantinel, L. S. Fernandes, J. L. Giongo, R. P. Raffin, and V. C. Rech. “RESVERATROL INCLUSION COMPLEX WITH β-CYCLODEXTRIN (RCD): CHARACTERIZATION AND EVALUATION OF TOXICITY IN WISTAR RATS”. International Journal of Pharmacy and Pharmaceutical Sciences, vol. 9, no. 1, Jan. 2017, pp. 27-33, doi:10.22159/ijpps.2017v9i1.15399.

Issue

Vol 9, Issue 1, 2017

Section

Original Article(s)
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