ANTIBACTERIAL ACTIVITY OF BIOLOGICALLY SYNTHESIS SILVER NANOPARTICLES FROM LEAF EXTRACT OF ALANGIUM SALVIFOLIUM (L.F) WANG
Keywords:
Alangium salvifolium, AgNPs, Leaf extract, Antibacterial activityAbstract
Biologically Synthesis process of silver nanoparticles using plant extract is simple, cost-effective, and ecofriendly. In the present study, silver nanoparticles (AgNPs) were rapidly synthesized using Alangium salvifolium leaf extract. The nanoparticles obtained have been characterized with various techniques like UV-Visible spectrum, Scanning Electron Microscopy (SEM), Energy Dispersive Spectroscopy (EDAX), X-ray Diffraction (XRD) and Atomic Force Microscopy (AFM) and also tested antibacterial. These techniques showed the formations of AgNPs with an average size of 24.2 to 52.5 nm and also find the spherical shaped nanoparticles. The synthesized AgNPs had the potential to mitigate the bacterial proliferation against Staphylococcus aureus, Pseudomonas auroginosa, Bacillus thuringiensis, Escherichia coli, Klebsiella pneumonia and Salmonella typhi. The results were compared with the Ciprofloxacin as positive controls. It is concluded that the Alangium salvifolium is one of the best sources for synthesis of AgNPs and also showed good anti bacterial activity.
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Song JY, Kim BS. Rapid biological synthesis of silver nanoparticles using plant extracts. J Bioprocess Biosyst Eng 2009;32:79-84.
Morones JR, Elechiguerra JL, Camacho A, Holt K, Kouri JB, Ramirez JT, et al. The bactericidal effect of silver nanoparticles. J Nanotechnlogy 2005;16:2346-53.
Shankar SS, Rai A, Ahmad A, Sastry MJ. Rapid synthesis of Au, Ag and Bimetallic Aushell nanoparticles using Neem. J Colloid Inter Sci 2004;275:496-502.
Sivakumar P, Sivakumar P, Anbarasu K, Padian K, Renganathan S. Synthesis of silver nanorods from Food industrial Waste and their application in improving the keeping quality of Milk. J Int Eng Chem Press 2013, 52:17676-81.
Nodwory PL, Wang J, Tredget EE, Burrell RE. Anti-inflammatory activity of nanocrystalline silver – derived solutions in porcine contact dermatitis. J Inflammation 2010;7:13.
Rogers JV, Parkinson CV, Choi YW, Speshock JL, Hussain SM. A preliminary assessment of silver nanoparticles inhibition of monkey pox virus plaque formation. J Nano Scale Res Let 2008, 3:129-33.
Shrivastava S, Bera T, Roy A, Sing G, Ramachandra R P, Dash D. Characterization of enhanced antibacterial effects of novel silver nanoparticles. J Nanotechnology 2007;18:225103.
Yu H, Xu X, Chen X, Lu T, Zhang P, Jing X. Preparation and antibacterial activity of Acticoat antimicrobial barrier dressing. J Burn Care Rehabil 2007;20:195-200.
Tsai CY, Shiau AL, Chen SY, Chen YH, Cheng PC, Chang MY, et al. Amelioration of collagen-induced arthritis in rats by nanogold. J Arthritis Rheum 2007, 56:544-54.
Mary SC, Murugan K, Mathath Roni, Sivapriya Jothi S, Suganya NA. Larvicidal potential of silver nanoparticles synthesized using Adiantum capillus verenis against anopheles stephensi. Int J Curr Trop Medic Health Res 2013;1:009-0158.
Linga Rao M, Savithramma N. Biological synthesis of silver nanoparticles using Svensonia hyderobadensis leaf extract and evaluation of their antimicrobial efficacy. J Pharm Sci Res 2011;3:1117-21.
Christensen L, Vivekanandhan S, Misra M, Mohanty A K. Biosynthesis of silver nanoparticles using Murraya koenigii (curry leaf):an investigation on the effect of broth concentration in reduction mechanism and particle size. J Adv Mat Lett 2011;2:429-34.
Ahmad N, Sharma S, Rai R. Rapid green synthesis of silver and gold nanoparticles using peels of Punica granatum. J Adv Mat Let 2012, 3:376-80.
Bhumi G, Savithramma N. Green synthesis of silver nanoparticles by Abrus precatorious Linn. Leaf extract and evaluation for antimicrobial activity. Int J of Drug and Development Res 2014;6.
Seralathan J, Stevenson P, Subramaniam S, Raghavan R, Pemaiah B, Sivasubramanian A, et al. Spectroscopy investigation on chemo-catalytic, free radical scavenging and bactericidal properties of biogenic silver nanoparticles synthesized using Salicornia brachiata aqueous extract. J Spectro Acta Part A:Mole Biomo Spectro 2014, 118:349-55.
Tariqo S, Javed A. Vitamin C content of Indian medicinal plants-A review literature. J Indian Drugs 1985, 23:72-5.
Dutta AK, Pakrashi SC. J Ann Biochem Exp Med 1963;23:285.
Jain A, Katewa SS, Galav PK, Sharma P J Ethnopharmacol 2005;102:143.
Chopra R N, Chowhan J S. Indian J Med Res 1934;21:507.
Sanyal AK, Dasgupta B, Das PK. Studies of Alangium lamarckii, Thw. I. Pharmacological studies of the total alkaloidal extract of the leaves. Indian J Med Res 1965;53:1055.
Sharstri MR. Herbal drugs. J Eastern Pharmacist 1993;421:49-52.
Bakhru, HC. Herbs that heal. J Orient Longman Ltd 1997;17.
Ray PC. History of chemistry in Ancient and Medical India. J Indian Chem Soc 1956;36.
Bauer AW, Kirby MM, Sherris JC, Turck M. Antibiotic susceptibility testing by a standardized single disk method. Am J Clin Pathol 1986;45:492-6.
Vivekanandhan S, Schreiber M, Mason C, Mohanty AK, Misra M. Maple leaf (Acer sp.) extract mediated green process for the fictionalization of ZnO powders with silver nanoparticles. J Coll Suf B Bioin 2014;113:169-75.
Lingarao M, Bhumi G, Savithramma N. Green synthesis of silver nanoparticles by Allamanda cathartica L. leaf extract and evaluation for antimicrobial activity. Int J Pharm Sci Nano 2013:4:2260-8.
Raether H. New York surface plasma oscillations and their applications. J In Physics of Thin Films 1997:9:145-71.
Xia Y, Halas NJ. Shape-controlled synthesis and surface plasmonic properties of metallic nanostructures MRS. J Bulletein 2005:30:338-43.
Panneerselvam C, Ponarulselvam S, Murugan K, Kalimuthu K,Thangamani S. Synthesis of silver nanoparticles using leaves of Catharanthus roseus Linn. G. Don and their antipla activities. Asian Pac J Trop Biomed 2012;7:574-80.
Ahmad N, Sharma S, Singh VN, Shamsi SF, Fatma A, Mehta BR. Biosynthesis of silver nanoparticles from Desmodium trifolium:A novel approach towards weed utilization. J Biotech Res Int 2011;1-8.
Savithramma N, Lingarao M, Ankanna S, Venkateswarlu P. Screening of medicinal plants for effective Biogenesis of silver nanoparticles and efficient antimicrobial activity. Int J Phar Sci Res 2012;3:1141-8.