ROLE OF SUSPENDED PARTICULATE MATTER IN ANGIOGENESIS EMPLOYING CROWN GALL TUMOR ASSAY
Keywords:Particulate matter, Carcinogenicity, Tumor-inducing ratio
Objective: Aim of the study was to screen the suspended particulate matter for their role in enhancing angiogenesis employing crown gall tumor assay.
Methods: Particulate matter (PM10) samples were collected, on glass fiber filter paper at the rate of 1.12â€“1.14 m3/min for 8 hr using high volume sampler, from six different sites of Amritsar city. Aqueous extracts of suspended particulate matter were prepared using a mechanical shaker for 24 hour and the filtrate was centrifuged and lyophilized.
Results: Maximum (34.67Â±1.764) tumors were induced by samples collected from Crystal chowk whereas tumor inducing ratio (TIR) was found to be comparably high for two sites viz., Crystal chowk and Bhandari bridge.
Conclusion: High tumor inducing ratio at the above-mentioned sites was correlated to high traffic emission from automobile exhaust. Crown gall tumor assay has been proved to be rapid, economical and reliable screening assay for angiogenesis agent.
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