SURVIVAL TIME AND HISTOLOGICAL OUTCOME OF MAJOR ORGANS FOLLOWING HONEY ADMINISTRATION TO ACUTE PARAQUAT-INTOXICATED RATS: A PRELIMINARY STUDY

Suk Peng Tang; Hasnan Jaafar; Siew Hua Gan; Kuttulebbai N.s. Sirajudeen; Siti Amrah Sulaiman

doi:10.22159/ijpps.2017v9i2.15466

Authors

Suk Peng Tang Department of Pharmacology, School of Medical Sciences, Health Campus, Universiti Sains Malaysia, 16150 Kubang Kerian, Kelantan, Malaysia
Hasnan Jaafar Department of Pathology, School of Medical Sciences, Health Campus, Universiti Sains Malaysia, 16150 Kubang Kerian, Kelantan, Malaysia
Siew Hua Gan Human Genome Center, School of Medical Sciences, Health Campus, Universiti Sains Malaysia, 16150 Kubang Kerian, Kelantan, Malaysia
Kuttulebbai N.s. Sirajudeen Department of Chemical Pathology, School of Medical Sciences, Health Campus, Universiti Sains Malaysia, 16150 Kubang Kerian, Kelantan, Malaysia
Siti Amrah Sulaiman Department of Pharmacology, School of Medical Sciences, Health Campus, Universiti Sains Malaysia, 16150 Kubang Kerian, Kelantan, Malaysia

DOI:

https://doi.org/10.22159/ijpps.2017v9i2.15466

Keywords:

Paraquat, Acute toxicity, Tualang honey

Abstract

Objective: The objective of this research was to investigate the possible protective effect of Tualang honey (TH) in acute paraquat (PQ) toxicity in rats.

Methods: A total of 48 male Sprague-Dawley rats aged eight weeks old were used. Oral PQ and TH were administered at 225 mg/kg and 0.2 g/kg, respectively. The effects of single and multiple TH treatmentson PQ-intoxicated rats were then investigated. Single TH treatment groups received TH at 0.5 (PQ+TH0.5h), 2 (PQ+TH2h) or 6 (PQ+TH6h) hours following PQ administration. Multiple TH treatment groups received TH at 0.5, 2 and 6 h (PQ+THtrp) or further daily treatment for the following six days (PQ+TH7d) after PQ administration (n=6 per group). The survival time of the rat was recorded until day 28 before sacrifice, which was followed by a histological examination.

Results: Treatment with TH did not improve the survival rate of PQ-intoxicated rats. However, the median survival time of rats that received multiple TH treatments was significantly longer compared to that of the PQ+TH6h group. TH treatment was found to improve the histological outcomes of PQ-intoxicated rats, particularly in the lungs.

Conclusion: Our findings suggest the potential role of honey in delaying the toxic effects of PQ.

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