STUDIES ON PREBIOTIC FOOD ADDITIVE (INULIN) IN INDIAN DIETARY FIBRE SOURCES - GARLIC (ALLIUM SATIVUM), WHEAT (TRITICUM SPP.), OAT (AVENA SATIVA) AND DALIA (BULGUR)
Keywords:
Dietary fibre, Prebiotic, Inulin, FTIR, TLC, HPLCAbstract
Objective: In the present investigation inulin has been extracted from dietary fibre rich Indian food stuffs, namely, garlic, wheat, oat and dalia. Inulin in the raw food stuff and in the extract has been assessed qualitatively and quantitatively.
Methods: Inulin has been extracted from each food source using a combination of lab-scale chemical processes and unit operations. Qualitative assessment of inulin in different food samples and their extracts has been done using FTIR and TLC and quantitative assessment has been done using high performance liquid chromatography (HPLC) and also through combination of TLC and spectro-photometry.
Results: The concentration (on dry weight basis) of inulin in natural prebiotic sources has been determined to be 16.60%, 13.07%, 8.94%, 14.95% for garlic, wheat, oat and dalia respectively. The extraction of inulin from garlic, wheat, oat and dalia was possible up to the extent of 99.46%, 77.94%, 53.31% and 89.15% respectively.
Conclusion: Â It may be concluded that all the food samples, investigated under the study, may serve as potential sources for extraction of prebiotic inulin. The present extraction procedure may be escalated to commercial scale for the production of inulin particularly from garlic for which the efficiency is as high as 99.46%.
Downloads
References
Vankerckhoven VV, Autgaerden TV, Huys G, Vancanneyt M, Swings J, et al. Establishment of the Prosafe Collection of Probiotic and Human Lactic acid Bacteria. Microb Ecol Infect Dis 2004;16:131-6.
Boeni S, Pourahmad R. Use of inulin and probiotic Lactobacilli in synbiotic yogurt production. Ann Biol Res 2012;3(7):3486-91.
Arihara RK, Ota HR, Itol M, Sameshima Y, Yamanaka H, Akimoto M, Kanai S, et al. Lactobacillus acidophilus group Lactic-acid bacteria applied to meat fermentation. J Food Sci 1998;63(3): 544-7.
Oliveira RP DS, Perego P, Converti A, Oliveira MND. Effect of inulin on growth and acidification performance of different probiotic bacteria in co-cultures and mixed culture with Streptococcus thermophilus. J Food Engineering 2009;91(1):133-9.
Rodrigues D, Teresa AP, Santos R, Pereira CI, Gomes AM, Malcata FX, et al. The potential effect of FOS and inulin upon probiotic bacterium performance in curdled milk matrices. LWT Food Sci Technology 2011;44(1):100-8.
Huebner J, Wehling RL, Hutkins RW. Functional Activity of commercial probiotics. Int Dairy J 2007;17:770-5.
Mantzouridou F, Spanou A, Kiosseoglou V. An Inulin-based dressing emulsion as a potential probiotic food carrier. Food Res Int 2012;46:260-9.
Parada JL, Caron CR, Adriane BP, Medeiros, Soccol CR. Bacteriocins from Lactic acid-bacteria: Purification. Brazilian Archives Biology and Technology 2002;522.
Roberfroid MB. Inulin-type fructans: functional food ingredients. J Nut 2007;137Suppl 11:2493S-502S.
Abrams S, Griffin I, Hawthome K, Liang L, Gunn S, Darlington G, et al. A combination of prebiotic short-and long-chain inulin-type fructans enhances calcium absorption and bone mineralization in young adolescents. Am J Clin Nutr 2005;82(2):471–6.
Kaur N, Gupta AK. Applications of inulin and oligofructose in health and nutrition: review. J Bio Sci 2002;27:703–14.
Tungland BC. Inulin: a comprehensive scientificreview. http: //members.shaw.ca/duncancrow/inulinreview.html. 2000.
Simonovska B. Determination of Inulin in Foods. J AOAC Int 2000;83:3.
Teris A VB, Paul M, Bonnie MK, Edith L, Alphons GJ, Voragen, Aede DG. Bitter sesquiterpene lactones from chicory roots. J Agric Food Chem 1990;38:1035-8.
Druart N, De Roover J, Van den Ende W, Goupil P, Van Laere A, Rambour S. Sucrose assimilation during early developmental stages of chicory (Chicorium intybus L.) plants. Planta 2001;212:436-43.
Mavumengwana VB. Isolation Purification and Characterisation of inulin and fructo-oligosaccharides from Chicorium intybus and inulinase from Aspergillus niger. Thesis for the degree of M. Sc. Department of Biochemistry, Microbiology and Biotechnology; Rhods University: 2004.
Singh Marmit K, Sharma SL. Quantitative estimation of some metabolites and enzymes in insect induced leaf galls of Mangifera indica. Asian J Exp Sci 2008;22(3):343-6.
www.senseair.se/senseair/gases-applications/hydrocarbons.
Cardenas GT, Salinas S. TGA-FTIR of PdSn Fine solid supported on MgO. cyclohexene reduction. J Chilean Chem Soc 2004;49(3):267-71.
Burn DA, Qurczak EW (Eds). Handbook of Near infrared Analysis. Practical Spectroscopy series, Marcel Dekker, Inc, NewYork, 1992;13:393-5.
wikis.lawrence.edu/display/chem.
Dinixchemy.Jumatdinintahyontahdakblogspot.in/2012/12/v, 2012.
Milorad D, Cakic, Goran SN, Ljubomir A I. FTIR Spectra of Iron (III) complexes with dextran, pullulan and inulin oligomers. Bull Chem Technol Maced 2002;21(2):135-46.