PREPARATION AND CHARACTERISATION OF IBUPROFEN LOADED POLYMERIC NANOPARTICLES BY SOLVENT EVAPORATION TECHNIQUE
Keywords:
Nanoparticles, Ethyl cellulose, Eudragit, Solvent evaporation, Drug releaseAbstract
Objective: In the present study Ibuprofen loaded nanoparticles were prepared by solvent evaporation technique. Comparative study was done among the formulations to know the best stabilizer and the polymer for ibuprofen loaded nanoparticles.
Methods: Four formulations F1, F2, F3 and F4 were prepared by solvent evaporation technique by changing the polymers and the stabilizers. The polymers used were Ethyl cellulose and Eudragit S-100 and the stabilizers were tween 80 and 0.4% PVA.The drug and the polymers was dissolved in solvent mixture of DCM and methanol in 1:1 ratio and added to the aqueous stabilizers. Comparative study was done to know the better formulation for EC with 0.4% PVA and tween 80, and also for Eudragit S-100 with Tween 80 and 0.4%PVA.Further Comparative study was performed between the best formulation of EC (F2) and Eudragit S-100 (F3).The effect of stabilizer and the polymer on nanoparticle size, stability, loading capacity, encapsulation efficiency and In vitro drug release was studied.
Results: Ibuprofen nanoparticles.F1,F2,F3 and F4 were prepared by using EC with tween 80 and 0.4% PVA and Eudragit with the same stabilzers. The particle size and stability of the formulations was determined by Horiba SZ 100 series particle size analyzer. Encapsulation efficiency and loading capacity were determined by ELTEK NP 400 Ultracentrifuge. The drug content was found to be 87%,88.72%,81.05% and 80.6%,encapsulation efficiency was found to be 56.25%,88.36%,71.7% and 55.9 %,loading capacity was found to be 28.8%,39.2%,33% and 28.4% respectively for F1,F2,F3 and F4. The average particle size was found to be 320.9 nm for F2 and 694.8 nm for F3.The zeta potential was found to be optimum for both the formulations.
Conclusions: On comparison F2 was considered to be the best formulation for the preparation of Ibuprofen loaded nanoparticles because of the smaller particle size and greater stability, greater loading capacity and encapsulation efficiency. The drug release was sustained till 8 hrs for nanoparticles in F2.
Downloads
References
Mohsen Jahanshahi, Zahra Babaei. Protein Nanoparticle. A unique system as drug delivery vehicle. Afr J Biotech 2008:4926-34.
Vyas SP. Khar RK. Targeted and Controlled Drug Delivery. Novel Carrier Systems. J CBS Publications 2002:331-87.
Jain NK. Advances in controlled and Novel Drug Delivery, 1st ed.CBS publishers and Distributers:New Delhi;2001.p. 408.
Vila A, Sanchez A, Tobio M, Calvo P, Alonso MJ. Design of biodegradable particles for protein delivery. J Control Release 2002;78.
Jelvehgari M, et al. Preparation and Evaluation of Poly (-caprolactone) Nanoparticles-in-Microparticles by W/O/W Emulsion Method. Iranian J of Basic Medical Sci 2010;13:1–8.
Herzfeld CD, Kummel R. Dissociation constant, solubility's and dissolution rate of some selective non steroidal anti inflammatory drugs. J Drug Dev Ind Pharm 1983;9:767-93.
Jain S, Saraf S. Influence of processing variables and In vitro characterization of glipizide loaded biodegradable nanoparticles. J Diabetes and Metabolic Syndrome Cl Res Rev 2009;3:113-7.
Wahbi AA, Hassan E, Hamdy D, Khamis E, Baray M. Spectrophotometric methods for the determination of Ibuprofen in tablets. Pak J Pharm Sci 2005;18.
Herzfeld CD, Kummel R. Dissociation constant, solubility's and dissolution rate of some selective non steroidal anti inflammatory drugs. J Drug Dev Ind Pharm 1983;9:767-93.
Soppimath KS. Aminabhavi TM. Kulkarni AR. Rudzinski W. Biodegradable polymeric nanoparticles as drug delivery device. J Controlled Release 2001:70:1-20.
Ritter JM, Lewis L, Mant TGK. A Text Book of Clinical Pharmacology. London:Arnold Ltd;1999.p. 365.
Witschi C, Doelker E. Influence of the microencapsulation method and peptide loading on poly (lactic acid) and poly (lactic-co-glycolic acid) degradation during In vitro testing. J Control Release 1998;51:327-41.
Trapani A, Sitterberg J, Bakowsky U, Kissel T. The potential of glycol chitosan nanoparticles as carrier for low water soluble drugs. Int J Pharm 2009;375:97-106.
Kopade AJ, Jain NK. Self assembling nanostructures for sustained ophthalmic drug delivery. J Pharmazie 1995;50:812-4.
Dongming P, Huang K, Liu Y, Lin S. Preparation of novel polymeric microspheres for controlled release of finasteride. Int J Pharm 2007;342:82-6.
Witschi C, Doelker E. Influence of the microencapsulation method and peptide loading on poly (lactic acid) and poly (lactic-co-glycolic acid) degradation during In vitro testing. J Control Release 1998;51:327-41.
Douglas JS, Davis S Douglas JS, Davis SS, Illum L. Nanoparticles in drug delivery. J Crit Rev Ther Drug Carrier Syst 1987;3:233-61.
S, Illum L. Nanoparticles in drug delivery. J Crit Rev Ther Drug Carrier Syst 1987;3:233-61.
Pignatello R, Amico D, Chiechio S, Giunchedi P, Spadaro C, Puglishi G. Preparation and analgesic activity of Eudragit RS100 microparticles containing diflunisal. J Drug Delivery 2001;8:35-45.
Perumal D, Dangor CM, Alcock RS, Hurbans N. Moopanar KR. Effect of formulation variables on In vitro drug release and micromeritic properties of modified release of ibuprofen microspheres. J Microencapsul 1999;16:475-47.