TOTAL PHENOLIC CONTENT, ANTIOXIDANT AND ANTICANCER ACTIVITIES OF FOUR SPECIES OF SENNA Mill. FROM NORTHEAST BRAZIL

Authors

  • Juliana Gaspar Alan E. Silva Department of Organic and Inorganic Chemistry
  • Jackeline Alves Monteiro Department of Organic and Inorganic Chemistry
  • Elayne Bessa Ferreira Department of Organic and Inorganic Chemistry
  • Maria Isabel Barros Fernandes Department of Analytical Chemistry and Physical-Chemistry, Department of Physiology and Pharmacology
  • CLAUDIA DOO PESSOA Federal University of Ceará, 60455-970, Fortaleza, Ceara, Brazil,
  • Caroline D. E. Goes Sampaio Department of Organic and Inorganic Chemistry, Federal Institute of Education, Science and Technology – IFCE, Maracanaú, Ceará, 61.939-140‎,
  • Maria Goretti D. E. Vasconcelos Silva Department of Organic and Inorganic Chemistry, Department of Analytical Chemistry and Physical-Chemistry, Department ofPhysiology and Pharmacology

Keywords:

Senna gardneri, Senna macranthera, Senna splendida, Senna trachypus, Antioxidant, Anticancer, Phenolic compound

Abstract

Objective: The present investigation evaluated the antioxidant and anticancer properties and total phenolic contents of four species of Senna: S. gardneri, S. macranthera, S. splendida and S. trachypus from northeast Brazil.

Methods: Ethanolic extracts of leaves and roots of the four Senna species were screened for phytochemical procedures. An in-vitro antioxidant study was conducted by means of DPPH and ABTS radical scavenging assays. Anticancer activity was evaluated using the MTT 3-(4,5-dimethylthiazole-2-yl)-2,5- diphenyltetrazolium bromide) method against the HCT-116, SF-295 and OVCAR-8 cancer cell lines. Total phenolic contents were determined using a Folin-Ciocalteau colorimetric assay.

Results: Anthraquinones, flavonoids, phenolics, steroids, tannins, triterpenoids, xanthones, and glycosides were detected in all Senna extracts. The results for the antioxidant activity showed that the highest percentage of scavenger radicals was present in the extract from roots of Senna trachypus (StR), which showed higher levels than the two standards used. The highest percentage of inhibition of the cancer cell lines tested was obtained with the leaf extracts of S. gardneri (SgL) and S. splendida (SsL).

Conclusion: The ethanolic extracts of the four species of Senna showed antioxidant activity in both assays, and this activity can be attributed to the presence of phenolic compounds such as flavonoids and anthraquinones. S. trachypus showed a higher antioxidant potential than the standards, as well as a higher total phenolic content. Only two of the plants showed promising results for anticancer activity.

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References

Silva CR, Monteiro MR, Rocha HM, Ribeiro AF, Caldeira-de-Araujo A, Leitão AC, et al. Assessment of antimutagenic and genotoxic potential of senna (Cassia angustifolia Vahl.) aqueous extract using in vitro assays. Toxicol in Vitro 2008;22:212–218.

Rao YK, Geethangili M, Fang S-H, Tzeng Y-M. Antioxidant and cytotoxic activities of naturally occurring phenolic and related compounds:a comparative study. Food Chem Toxicol 2007;45:1770–1776.

Oliveira AC, Valentim IB, Goulart MOF, Silva CA, Bechara EJH, Trevisan MTS. Vegetals as natural sources of antioxidants. Quim Nova 2009;32:689-702.

Costa-Lotufo LV, Montenegro RC, Alves A, Madeira SVF, Pessoa C. The Contribution of Natural Products as Source of New Anticancer Drugs:Studies Carried Out at the National Experimental Oncology Laboratory from the Federal University of Ceará. Rev Virtual Quím. 2010;2:47-58.

Parolin P. Senna reticulata, a pioneer tree from Amazonian várzea floodplains. In The Botanical Review, New York, Springer Ed.; 2000. p. 239-254.

Mokgotho MP, Gololo SS, Masoko P, Mdee LK, Mbazima V, Shai LJ, et al. Isolation and Chemical Structural Characterisation of a Compound with Antioxidant Activity from the Roots of Senna italica. Evid Based Complement Alternat Med 2013;1-6.

Sriphong L, Sotanaphun U, Limsirichaikul S, Wetwitayaklung P, Chaichantipyuth C, Pummangura S. Cytotoxic alkaloids from the flowers of Senna spectabilis. Planta Med 2003;69(11):1054-6.

Harbone JB, Phytochemical Methods:to Modern Techniques of Plant Analysis 2nd ed. Chapman and Hall: New York (NY), 1973.

Matos FJA. Fitoquímica Experimental 3rd ed. UFC: Fortaleza (CE) 2009..

Brand-Williams W, Cuvelier ME, Berset C. Use of a free radical method to evaluate antioxidant activity. LWTFood Sci Technol 1995;28:25-30.

Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic Biol Med 1999;26(9-10):1231-7.

Bonoli M, Verardo V, Marconi E, Caboni MF. Antioxidant phenols in barley (Hordeum vulgare L.) flour:comparative spectrophotometric study among extraction methods of free and bound phenolic compounds. J Agric Food Chem 2004;52(16):5195-200.

Skehan P, Storeng R, Scudiero D, Monks A, McMahon J, Vistica D, et al. New colorimetric cytotoxicity assay for anticancer-drug screening. J Natl Cancer Inst 1990;82(13):1107-12.

Broinizi PRB, Andrade-Wartha ERS, Silva AMO, Novoa AJV, Torres RP, Azeredo HMC. Evaluation of the Antioxidant Activity of Phenolic Compounds Naturally Contained in By-products of the Cashew Apple (Anacardium occidentale L. Cien Tecnol Aliment 2007;27:902-8.

Boulanouar B, Abdelaziz G, Aazza GS, Gago C, Miguel MG. Antioxidant activities of eight Algerian plant extracts and two essential oils. Ind Crops Prod 2013;46:85-96.

Saravanan BC, Sreekumar C, Bansal GC, Ray D, Rao JR, Mishra AK. A rapid MTT colorimetric assay to assess the proliferative index of two Indian strains of Theileria annulata. Vet Parasitol 2013;113:211-6.

Lin K-Y, Uen Y-H. Aloe-emodin, an anthraquinone, in vitro inhibits proliferation and induces apoptosis in human colon carcinoma cells. Oncol Lett 2010;1(3):541-7.

Published

01-07-2014

How to Cite

Silva, J. G. A. E., J. A. Monteiro, E. B. Ferreira, M. I. B. Fernandes, C. D. PESSOA, C. D. E. G. Sampaio, and M. G. D. E. V. Silva. “TOTAL PHENOLIC CONTENT, ANTIOXIDANT AND ANTICANCER ACTIVITIES OF FOUR SPECIES OF SENNA Mill. FROM NORTHEAST BRAZIL”. International Journal of Pharmacy and Pharmaceutical Sciences, vol. 6, no. 7, July 2014, pp. 199-02, https://journals.innovareacademics.in/index.php/ijpps/article/view/1728.

Issue

Vol 6, Issue 7, 2014

Section

Original Article(s)