SIMULTANEOUS QUANTITATIVE ESTIMATION OF MEBEVERINE HYDROCHLORIDE AND CHLORDIAZEPOXIDE IN CAPSULES USING SPECTROPHOTOMETRY
Keywords:
UV, Mebeverine hydrochloride, Chlordiazepoxide, Method development, ValidationAbstract
Objective: To develop a simple and cheap UV spectrophotometric method for the simultaneous quantitative estimation of Mebeverine hydrochloride and Chlordiazepoxide in MEVA C Capsules and validate as per ICH guidelines.
Methods: The optimized method uses triethylammonium phosphate buffer (pH 4.0) as a solvent for the estimation of assay of Mebeverine hydrochloride and Chlordiazepoxide in capsules at detection wavelengths of 263 nm and 245 nm respectively.
Results: The developed method exhibited linearity in the range of 7.5-22.5μg/ml for Mebeverine hydrochlorideand 2.5-7.5μg/ml for Chlordiazepoxide. Precision for Mebeverine hydrochloride and Chlordiazepoxide is exemplified by relative standard deviation of 0.589% and 0.332 respectively. Percentage Mean recovery for Mebeverine hydrochloride and Chlordiazepoxide was found to be in the range of 98â€102, during accuracy studies. The limit of detection (LOD) for Mebeverine hydrochloride and Chlordiazepoxide was found to be 528ng/ml and 192ng/ml respectively, while limit of quantitiation (LOQ) for Mebeverine hydrochloride and Chlordiazepoxide was found to be 1.6µg/ml and 583ng/ml respectively.
Conclusion: A simple and a cheap UV spectrophotometric method were developed and validated for the simultaneous quantitative estimation of Mebeverine hydrochloride and Chlordiazepoxide in capsules as per ICH guidelines and hence it can be used for the routine analysis in various pharmaceutical industries.
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References
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