BENEFICIAL EFFECTS OF ETHANOLIC LEAF EXTRACT OF CORIANDRUM SATIVUM ON METANIL YELLOW INDUCED ALTERATION INACTIVITY OF CATALASE AND LEVEL OF LIPID PEROXIDATION IN HERCINE CARDIAC TISSUE IN VITRO
DOI:
https://doi.org/10.22159/ijpps.2017v9i5.17331Keywords:
Cardioprotection, Coriandrum sativum, Metanil yellow, Methanolic leaf extract, Myocardial infarctionAbstract
Objective: The primary aim of the study was to find out a suitable effective dose of metanil yellow that can cause alterations of lipid peroxidation and catalase activity in the heart in vitro and to determine the effectiveness of graded doses of Coriandrum sativum as an antioxidant in preventing lipid peroxidative damage to cardiac tissue and back up catalase activity.
Methods: Metanil yellow at different doses (10, 25, 35, 50 µl in phosphate buffer) were applied on isolated hircine heart homogenate (10%) in in vitro followed by estimation of lipid peroxidation and catalase activity after completion of the incubation period. Another set of the experiment included incubation of the heart homogenates with graded dilutions of ethanolic leaf extracts of Coriandrum sativum (CsEth) at concentrations of 10, 20, 30, 40 µl in phosphate buffer medium followed by quantification of the same biochemical parameters as above. This was followed by co-administration of the selected effective dose of metanil yellow (35 µl) and ethanolic leaf extract of Coriandrum sativum (30 µl) on heart homogenate to track the variation in lipid peroxidation and catalase activity in heart homogenate in vitro.
Results: Metanil yellow significantly lowered catalase activity in isolated cardiac tissue that was reflected in elevated lipid peroxidation assuming free radical induced oxidative injury to cardiac tissue (p<0.01). However ethanolic leaf extract of Cordiandrum sativum buffered such changes (p<0.01) significantly, presuming a protective action against oxidative cardiac damage.
Conclusion: It may be concluded that ethanolic leaf extract of Coriandrum sativum is a promising cardioprotective remedy not only in mitigating free radical mediated lipid peroxidative damage to cardiac tissue but also in reinstating normal catalase activity that may be used an unique herbal based remedy for such cardiac ailments in future.
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References
Patel SP, Katyare SS. A comparative study of reactive oxygen species (ros) related parameters in rat tissues. Indian J Clin Biochem 2006;21:48-53.
Raman K, Rajni S. Review on rasayana and its relevance in the current scenario. Punarnav 2014;2:1-10.
Tsutsui H, Kinugawa S, Matsushima S. Oxidative stress and heart failure. Am J Physiol Heart Circ Physiol 2011;301:H2181-90.
Thayer WS. Role of catalase in the metabolism of hydrogen peroxide by the perfused rat heart. FEBS Lett 1986;202:137-40.
Hazra S, Dome R, Ghosh S, Ghosh D. Protective effect of methanolic leaves extract of coriandrum sativum against metanil yellow induced lipid peroxidation in goat liver: an in vitro study. Int J Pharmacol Pharm Sci 2016;3:34-41.
Gupta S, Sundarrajan M, Rao KV. Tumor promotion by metanil yellow and malachite green during rat hepatocarcinogenesis is associated with dysregulated expression of cell cycle regulatory proteins. Teratog Carcinog Mutagen 2003; Suppl 1:301-12.
Al-Malki AL, Sayed AAR. Bees' honey attenuation of metanil-yellow-induced hepatotoxicity in rats. J Evidence-Based Complementary Altern Med 2013. http://dx.doi.org/10.1155/ 2013/614580.
Saha MR, Hasana SMR, Aktera R, Hossaina MM, Alam MS, Alam MA, et al. In vitro free radical scavenging activity of methanol extract of the leaves of Mimusops elengi linn. Bangl J Vet Med 2008;6:197–202.
http://www.ayushveda.com/herbs/coriandrum-sativum.htm [Last accessed on 16 Feb 2017]
http://www.coriandrumsativum.com/coriandrum-sativum-medical-uses/ [Last accessed on 16 Feb 2017]
Bogavac M, Karaman M, Janjušević L, Sudji J, Radovanović B, Novaković Z, et al. Alternative treatment of vaginal infections in vitro antimicrobial and toxic effects of Coriandrum sativum L. and Thymus vulgaris L. essential oils. J Appl Microbiol 2015;119:697-710.
Mani V, Parle M, Ramasamy K, Abdul Majeed AB. Reversal of memory deficits by Coriandrum sativum leaves in mice. J Sci Food Agric 2011;91:186-92.
Harsha SN, Anilakumar KR. In vitro free radical scavenging and DNA damage protective property of Coriandrum sativum L. leaves extract. J Food Sci Technol 2014;51:1533-9.
Reddy LH, Jalli RD, Jose B, Gopu S. Evaluation of antibacterial and DPPH radical scavenging activities of the leaf extracts and essential oil of Coriandrum sativum Linn. World J Pharm Res 2012;1:705-16.
Mandal S, Mandal M. Coriander (Coriandrum sativum L.) essential oil: Chemistry and biological activity. Asian Pacific J Trop Biomed 2015;5:421–8.
Kuganathan N, Saminathan S, Muttukrishna S. Toxicity of Datura alba leaf extract to aphids and ants. Int J Toxicol 2008;5:1-6.
Shrivastava S, Leelavathi S. Preliminary phytochemical evaluation of leaf extracts of Catunaregum spinosa Thunb. Int J Pharm Sci Rev Res 2010;3:114–8.
Paul S, Ghosh D, Ghosh AK, Dey M, Chattopadhyay A, Bandyopadhyay D. Lead induces oxidative stress in rat heart and liver tissue homogenates: an in vitro study. J Cell Tissue Res 2013;13:3829-37.
Ghosh D, Paul S, Naaz S, Bhowmik D , Dutta M, Ghosh AK, Firdaus SB, Chattopadhyay A, Reiter RJ, Bandyopadhyay D. Melatonin protects against lead acetate induced oxidative stress-mediated changes in morphology and metabolic status in rat red blood cells: a flow cytometric and biochemical analysis. J Pharm Res 2016;10(6):381-402.
Buege JA, Aust SG. Microsomal lipid peroxidation. Methods Enzymol 1978;52:302–10.
Beers Jr RF, Sizer IW. A spectrophotometric method for measuring the breakdown of hydrogen peroxide by catalase. J Biol Chem 1952;195:133-40.
Mitra E, Ghosh AK, Ghosh D, Mukherjee D, Chattopadhyay A, Dutta S, et al. Protective effect of aqueous curry leaf (Murraya koenigii) extract against cadmium-induced oxidative stress in rat heart. Food Chem Toxicol 2012;50:1340-53.
Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the Folin phenol reagent. J Biol Chem 1951;193:265-75.
Kang J, Chen Y, Epstein PN. Suppression of doxorubicin cardiotoxicity by overexpression of catalase in the heart of transgenic mice. J Biol Chem 1996;271:12610-6.
Kirshenbaum LA, Singal PK. Increase in endogenous antioxidant enzymes protects hearts against reperfusion injury. Am J Physiol 1993;265:H484-93
Ramchandani S, Das M, Joshi A, Khanna SK. Effect of oral and parenteral administration of metanil yellow on some hepatic and intestinal biochemical parameters. J Appl Toxicol 1997;17:85–91.
Chaudhary KR, Batchu SN, Seubert JM. Cytochrome P450 enzymes and the heart. IUBMB Life 2009;61:954–60.
Saravanan G, Prakash J. Effect of garlic (Allium sativum) on lipid peroxidation in experimental myocardial infarction in rats. J Ethnopharmacol 2004;94:155-8.
Blokha VV, Kagan VY, Sitkovskii MV, Danilov VS, Kols OR, Koslov YP. Peroxidation of lipids and conduction of excitation in frog muscles. Biofizika 1972;17:549-51.
Janero DR1, Hreniuk D, Sharif HM. Hydrogen peroxide-induced oxidative stress to the mammalian heart muscle cell (cardiomyocyte): lethal peroxidative membrane injury. J Cell Physiol 1991;149:347-64.
Priscilla DH, Prince PSM. Cardioprotective effect of gallic acid on cardiac troponin-T, cardiac marker enzymes, lipid peroxidation products and antioxidants in experimentally induced myocardial infarction in Wistar rats. Chem Biol Interact 2009;179:118–24.
Nirmala C, Puvanakrishnan R. Effect of curcumin on certain lysosomal hydrolases in isoproterenol-induced myocardial infarction in rats. Biochem Pharmacol 1996;51:47-51.
Susila R, Jeeva Gladys R, Balagurusamy K, Mubarak A. Review of siddha cardiology and cardioprotective herbs. Int J Herbal Med 2013;1:71-5.
Babu BH, Shylesh BS, Padikkala J. Antioxidant and hepatoprotective effect of acanthus ilicifolius. Fitoterapia 2001; 72:272-7.
Prior RL, Cao GH, Martin A, Sofic E, McEwen J, O’Brien C, et al. Antioxidant capacity as influenced by total phenolics and anthocyanin content, maturity, and a variety of Vaccinium species. J Agric Food Chem 1998;46:2686–93.
Braughler JM, Duncan CA, Chase LR. The involvement of iron in lipid peroxidation. Importance of ferrous to the ferric ratio in initiation. J Biol Chem 1986;261:10282-9.
Morelli R, Russo-Volpe S, Bruno N, Scalzo RL. Fenton-dependent damage to carbohydrates: free radical scavenging activity of some simple sugars. J Agric Food Chem 2003;51:7418–25.
Mitra E, Ghosh D, Ghosh AK, Basu A, Chattopadhyay A, Pattari SK, et al. Aqueous tulsi leaf (Ocimum sanctum) extract possesses antioxidant properties and protects against cadmium-induced oxidative stress in rat heart. Int J Pharm Pharm Sci 2014;6:500-13.
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