GC-MS PROFILE OF IN VIVO AND IN VITRO SHOOTS OF CLEOME GYNANDRA L.
DOI:
https://doi.org/10.22159/ijpps.2017v9i11.17351Keywords:
GC-MS, C gynandra, In vivo and in vitro plants, Biological activityAbstract
Objective: Investigation of the bioactive compounds from the ethanol shoot extracts of in vivo and in vitro plants of Cleome gynandra (C. gynandra) through GC-MS analysis.
Methods: The nodal explants were cultured on Murashige and Skoog (MS) medium supplemented with different concentrations of 6-benzyl-aminopurine (BAP), kinetin-6-furfurylaminopurine (Kin) and indole 3 acetic acids (IAA) for shoot induction. In the present study, the phytochemical constituents were analyzed from the ethanol extract of in vivo and in vitro plants of C. gynandra using Gas Chromatography-Mass Spectrometry (GC-MS) analysis. The mass spectrum of the ethanol extract was compared with the available library sources.
Results: In the present study, different concentrations of cytokinins and in the combination of IAA are used to develop regenerated shoots. The maximum number of shoots was obtained 9.2±0.41 with the length of 6.6 cm and highest frequency of (100%) shoot induction was observed on MS medium containing 10 μM BAP with 4 μM IAA. The GC-MS analysis revealed that the shoots of in vivo and in vitro plants contained 21phytochemicals, of these 3 components were similar in both in vivo and in vitro plants, 2 phytochemical's are repeated with different RT, 7 components are having biological activity and in the remaining 9 components, biological activities are not reported.
Conclusion: The present study, the in vitro regeneration, combinations of hormones (10 μM BAP plus 4 μM IAA) tested showed the best result than individual and also revealed that the synthesis of more number of phytochemicals present in the ethanolic extracts of in vitro plants than the in vivo plants of C. gynandra.
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References
Kuhn U. Capparaceae in Dicot Weed. TJ Hafliger, M Wolf. eds. CIBA Geigy LTD, Basle, Switzerland; 1988. p. 109-20.
Karthikeyan AVP, Shanmuganathan T, Karthi C. In vitro propagation of Cleome gynandra L. through direct organogenesis. Int J Sci Res 2014;3:1368-72.
Shanmuganathan T, Karthikeyan AVP. Antimicrobial activity of in vivo and in vitro plants of Cleome gynandra L. World J Pharm Pharm Sci 2016;5:948-53.
Ammara H, Salma R, Farah D, Shahid M. Antimicrobial activity of some plant extracts having hepatoprotective effects. J Med Plant Res 2009;3:20-3.
Chweya JA. Genetic enhancement of indigenous vegetables in Kenya. Paper presented at the workshop ‘Genetic Resources of Traditional Vegetables in Africa: Options for Conservation and Use. Nairobi Kenya 1995;8:29-31.
Babu KN, Minoo D, Geetha SP, Sumanthi V, Praveen K. Biotechnology of turmeric and related species. In: PN Ravindran, KN Babu, K Sivaraman. Eds. Turmeric: The genus Curcuma CRC Press: Boca Raton; 2007. p. 107-27.
Alfermann A, Petersen M, Fuss E. Production of natural products by plant cell biotechnology: results, problems and perspectives. In: M Lamier, W Rucker. Eds. Plant Tissue Culture 100 Y Since Gottlieb Haberlandt. Springer, New York; 2003. p. 153-66.
Preil W. A personal reflection on the use of liquid media for in vitro culture. In: A Hvoslef-Eide, W Preil. Eds. Liquid systems for in vitro plant propagation. Springer, Dordecht; 2005. p. 1-18.
Mary Sujin R, John De Britto A. Conservation of Vitex trifolia L. through in vitro Micropropagation. Int J Appl Biores 2013;15:35-8.
Aanchal Jain, Shashank Bhatt, Suresh Dhyani. Phytochemical screening of secondary metabolites of Ziziphus mauritiana Lam. Bark. Int J Curr Pharm Res 2012;4:156-9.
Nesy EA, Jose Padikkala, Lizzy Mathew, In vitro plant regeneration of Thevetia neriifolia Juss. from internode explants via indirect organogenesis. Int J Pharm Pharm Sci 2015;7:169-72.
Chukwuka KS, Ikheloa JO, Okonko IO, Moody JO, Mankinde TA. The antimicrobial activities of some medicinal plants on Escherichia coli as the agent of diarrhea in livestock. Adv Appl Sci Res 2011;2:37-48.
Bala A, Haldar PK, Kar B, Naskar S, Saha P, Kundusen S, et al. Antioxidant activity of the fractions of Cleome gynandra promotes antitumor activity in Ehrlich ascites Carcinoma. Asian J Chem 2011;23:5055-60.
Karimulla Shaika, Afsar Shaika, Deepak Kumar B, Devi Kadirvel. Evaluation of preliminary phytochemical properties and hypoglycemic activity of Cleome gynandra L. Int J Pharm Pharm Sci 2013;5:824-8.
Ajaiyeoba EO. Phytochemical and antimicrobial studies of Gynandropsis gynandra and Buchholzia coriaceae extracts. Afr J Biomed 2000;3:161-5.
Meda NTR, Bangou MJ, Bakasso S, Millogo-Rasolodimby J, Nacoulma OG. Antioxidant activity of phenolic and flavonoid fractions of Cleome gynandra and Maerua angolensis of Burkina Faso. J Appl Pharma Sci 2013;3:36-42.
Shanmuganathan T, Karthikeyan AVP. Phytochemical analysis and antioxidant activities of in vivo and in vitro shoots of Cleome gynandra L. Int J Sci Res 2016;5:208-11.
Mule SN, Patil SB, Naikwade NS, Magdum CS. Evaluation of the antinociceptive and anti-inflammatory activity of stems of Gynandropsis pentaphylla Linn. Int J Green Pharm 2008;2:87-90.
Wink M. Introduction: biochemistry, role and biotechnology of secondary products in the biochemistry of secondary product metabolism. Ed. Wink M. CRC Press: Boca Raton, FL; 1999;1:16-20.
Murashige T, Skoog F. A revised medium for rapid growth and bioassay with tobacco tissue cultures. Physiol Plant 1962;15:473-97.
Muhammad Tayyab, Durre Shahwar. GC-MS analysis of Cannabis sativa L. from four different areas of Pakistan. Egyptian J Forensic Sci 2015;5:114–25.
Mooza Al-Owaisi, Nora Al-Hadiwi, Shah Alam Khan. GC-MS analysis, determination of total phenolics, flavonoid content and free radical scavenging activities of various crude extracts of Moringa peregrina (Forssk.) Fiori leaves. Asian Pac J Trop Biomed 2014;4:964-70.
Dr. Duke's Phytochemical and Ethnobotanical Databases. Available from: URL: http://www.ars-grin.gov/duke. [Last accessed on 20 Dec 2016].
Ghanti K, Kaviraj CP, Venugopal RB, Jabeen FTZ, Rao S. Rapid regeneration of Mentha piperita L. from shoot tip and nodal explants. Indian J Biotech 2004;3:594-8.
Joshi P, Dhawan V. Axillary multiplication of Swertia chirayita (Roxb. Ex Fleming) H. Karst., a critically endangered medicinal herb of temperate Himalayas. In Vitro Cell Dev Biol Plant 2007;43:631-8.
Purkayastha J, Sugla T, Paul A, Solleti S, Sahoo L. Rapid in vitro multiplication and plant regeneration from nodal explants of Andrographis paniculata: a valuable medicinal plant. In Vitro Cellular Dev Biol-Plant 2008;44:442-7.
Thomas TD, Shankar S. Multiple shoot induction and callus regeneration in Sarcostemma brevistigma Wight and Arnott, a rare medicinal plant. Plant Biotech Rep 2009;3:67-74.
Baskaran P, Jayabalan N. In vitro mass propagation and diverse callus orientation on Sesamum indicum L.-an important oil plant. J Agric Tech 2006;2:259-69.
Loganathan K, Narmatha Bai V. High frequency in vitro plantlet regeneration and antioxidant activity of Enicostema axillare (Lam.) Raynal ssp. littoralis (Blume) raynal an important medicinal plant. Asian Pacific J Repro 2014;3:241-8.
Shrivastava N, Rajani M. Multiple shoot regeneration and tissue culture studies on Bacopa monnieri (L.) Pennell. Plant Cell Rep 1999;18:919-23.
Rahman MM, Ankhi UR, Biswas A. Micropropagation of Mentha viridis L.: An aromatic medicinal plant. Int J Pharm Life Sci 2013;4:2926-30.
Chandra Gurnani, Vikram Kumar, Shinam Mukhija, Abha Dhingra, Sohan Rajpurohit, Poonam Narula. In vitro regeneration of Brahmi (Bacopa monneiri (l.) Penn.)-a threatened medicinal plant. Kathmandu University J Sci Eng Technol 2012;8:97-9.
Ganesan CM, Paulsamy S. Mass propagation of an economically important medical plant, Lobelia nicotianaefolia Heyne using in vitro culture technique. Nat Prod Int J 2102;8:35-40.
Bhat MA, Mujib A, Junaid A, Mohamooduafar M. In vitro regeneration of Solanum nigrum with enhanced solasodine production. Biol Plant 2010;4:757-60.
Krishnan P, Kruger NJ, Ratcliffe RG. Metabolite fingerprinting and profiling in plants using NMR. J Exp Bot 2004;56:255-65.
Schauer N, Fernie AR. Plant metabolomics: towards biological function and mechanism. Trends Plant Sci 2006;11:508-16.
Anbazhagi K, Kadavul G, Suguna A, Petrus JA. Studies on the pharmacognostical and in vitro antioxidant potential of Cleome gynandra Linn. Leaves. Natl Prod Radiance 2009;8:151-7.
Prameela J, Ramakrishnaiah H, Krishna V, Deepalakshmi AP. GC-MS analyses of leaf and root extracts of Didymocarpus tomentosa. Int J Pharm Pharm Sci 2015;7:423-5.
Ruthiran Papitha, Lokesh Ravi, Chinnadurai Immanuel Selvaraj. Phytochemical studies and GC-MS analysis of spermadictyon suaveolens roxb. Int J Pharm Pharm Sci 2017;9:143-9.
Maruthupandian A, Mohan VR. GC-MS analysis of some bioactive constituents of Pterocarpus marsupium Roxb. Int J Chem Tech Res 2011;3:1652-7.
Sermakkani M, Thangapandian V. GC–MS analysis of Cassia italica leaf methanol extract. Asian J Pharma Clin Res 2012;5:90-4.
Ashwani Kumar, Surender Singh, Sandeep Jai, Parvin Kumar. Synthesis antimicrobial evaluation, QSAR and in silico ADMET studies of decanoic acid derivatives. Acta Poloniae Pharm Drug Res 2011;68:191-204.
Raja Rajeswari N, Rama Lakshmi S, Muthuchelian K. GC-MS analysis of bioactive components from the ethanolic leaf extract of Canthium dicoccum (Gaertn.) Teijsm and Binn. J Chem Pharm Res 2011;3:792-8.
Hema R, Kumaravel S, Alagusundaram K. GC/MS determination of bioactive components of Murraya koenigii. J Am Sci 2011;7:80-3.
Srinivas R, Jyothi Chaitanya P, Chandrashekar R, Lakshmi Bhavani N. Analysis of preliminary phytochemicals of leaf extracts of Cleome gynandra L. World J Pharm Sci 2014;2:1043-5.
Syeda FA, Habib-Ur-Rehman, Choudhary MI, Atta-Ur-Rahman. GC–MS analysis of petroleum ether extract (oil) and bioassays of crude extract of Iris germanica. Int J Gen Mole Biol 2011;3:95-100.
