THE STUDY OF ANTI-INFLAMMATORY AND ANTIOXIDANT ACTIVITY IN COLD PRESS RICE BRAN OIL FROM RICE IN THAILAND
Keywords:
Anti-inflammatory, Antioxidant activities, Oryza Sativa L, Nitic OxideAbstract
Objective: The present study is investigation in the rice bran oil from four rice varieties in term of anti-inflammatory and antioxidant activities.
Methods: This research studied the effects of anti-inflammatory and antioxidant activity from cold press rice bran oil. For anti-inflammatory, their inhibitory activity of nitric oxide (NO) production using RAW267.4 cell lines was evaluated. And two methods for antioxidant activities, DPPH radical scavenging assay (DPPH assay) and Ferric Reducing Antioxidant Power (FRAP) assay were used and compared with gallic acid standard and ferric sulfate (FeSO4), respectively.
Results: For the Oryza Sativa L. Khaw-khaw exhibited the highest activity against the NO production with an IC50 value of 41.96 μg/ml, followed by O. Sativa L. Hom Pathum (46.58 μg/ml), O. Sativa L. Hom Mali (53.84 μg/ml) and O. Sativa L. Hom Mali Gorkho (59.43 μg/ml). However, the antioxidant activity, DPPH method found O. Sativa L. Hom Mali Gorkho displayed the most potent effect with IC50 value of 0.08 mg/ml, followed by O. Sativa L. Hom Pathum (0.11mg/ml), O. Sativa L. Hom Mali (0.12mg/ml) and O. Sativa L. Khaw khaw (0.88 mg/ml), respectively. The assay of FRAP showed the highest in O. Sativa L. Hom Mali Gorkho with an IC50 value 2.27 mg/ml, followed by O. Sativa L. Hom Pathum (4.30 mg/ml), O. Sativa L. Khaw khaw (6.67 mg/ml) and O. Sativa L. Hom Mali (7.68 mg/ml), respectively.
Conclusion: This study indicated that cold press rice bran oil from rice varieties in Thailand is responsible for anti-inflammatory and antioxidant activity. Therefore, this study supports the tradition use of cold press rice bran oil for treatment of inflammatory related diseases though the inhibition of nitric oxide release.
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