EFFECT OFSALIX TETRASPERMAROXBUGH ON FRUCTOSE INDUCED HYPERTENSION IN RATS
DOI:
https://doi.org/10.22159/ijpps.2017v9i9.18232Keywords:
NIBP, Antihypertensive, Fructose, Glibenclamide, Salix tetrasperma RoxburghAbstract
Objective: The present study was designed to evaluate the effect of the ethanolic and aqueous extract of Salix tetrasperma Roxburgh on blood pressure by fructose induced hypertensive rats.
Methods: The Salix tetrasperma Roxburgh leaves were evaluated for antihypertensive potential by using fructose–induced hypertension model in Wister albino rats. The test animals were given high fructose (10%) diet for 21st days to induced hypertension. Subsequently, the 200 and 400 mg/kg/day (p. o.) doses of ethanolic and aqueous extracts of Salix tetrasperma leaves were administered to the different groups of hypertensive and normal animals. The hypertensive condition of the experimental animals was confirmed on 21st day by measuring systolic, diastolic and mean arterial pressure (SBP, DBP, MAP) using noninvasive BP (NIBP) system for rodents. The SBP, DBP, MAP were again recorded on day 0 d, 7th, 14th and 21st day of administration standard and test extracts. The normal control group of animals were given normal diet and administrated normal saline throughout the experiment.
Results: The both test extracts significantly reduced SBP, DBP and MAP significantly (P<0.05) lowered blood pressure effect in 0 d, 7th day at the dose of 200 and 400 mg/kg in fructose induced hypertensive rats. On 14thday the test extracts at the doses of 400 mg/kg significantly reduce only DBP and MAP. However, the treatment is continued for 21 d but no significant activity observed. The test extracts reveals that antihypertensive of Salix tetrasperma Roxburgh in dose dependent manner in hypertensive control rats.
Conclusion: These observations established the traditional claim and thus Salix tetrasperma Roxburgh could be a potent antihypertensive agent for use in future. The phyto constituents present in the test samples may be responsible for the hypotensive effect. However, further investigation is required to identify the active principles responsible for antihypertensive activity.
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References
Parks EJ, Hellerstein MK. Carbohydrate-induced hyper triacyl-glycerolemia: historical perspective and review of biological mechanisms. Am J Clin Nutr 2000;71:412-33.
Fried SK, Rao SP. Sugars, hypertriglyceridemia and cardiovascular disease. Am J Clin Nutr 2003;78:873-80.
Badyal DK, Lata H, Dadhich AP. Animal models of hypertension and effect of drugs. Indian J Pharmacol 2003;35:349-62.
Deep Raj Paul, Kartika Paul. Diabetes mellitus and its complications: a review. Int J Curr Pharm Res 2012;4:12-7.
Noumi E, Houngue F, Lontsi D. Traditional medicines in primary health care: plants used for the treatment of hypertension in Bafia, Cameroon. Fitoterapia 1999;70:134-9.
Barnes PM, Powell-Griner E, McFann K, Nahin RL. Complementary and alternative medicine use among adults: United States, 2002. Seminars Integrative Med 2004;2:54-71.
Bishop FL, Yardley L, Lewith GT. A systematic review of beliefs involved in the use of complementary and alternative medicine. J Health Psychol 2007;12:851-67.
OECD Guidelines for the Testing of Chemicals, Acute oral Toxicity-Up-and-Down-Procedure (UDP), OECD/OCDE 425; 2008. Available from: http://ntp.niehs.nih.gov/?objectid= 62883FD5‑09D2‑26AC‑F2ED08869156822B. [Last accessed on 10 Oct 2016].
Kulkarni SK. Handbook of Experimental Pharmacology. 3rd Ed. New Delhi: Vallabh Prakashan; 2002.
Alamgeer. Antihypertensive activity of aqueous methanol extract of Berberis Orthobotrys Bien ex. aitch in rats. Trop J Pharm Res 2013;12:393-9.
Ika Purwidyaningrum, Elin Yulinah Sukandar, Irda Fidrianny. Antihypertensive activity of extract and fractions of Matoa (pometia pinnata j. R and g forts) leaves. Asian Pharm Clin Res 2017;10:323-8.
Hwang IS, HHO, BB Hoffman, GM Reaven. Fructose-induced insulin resistance and hypertension in rats. Hypertension 1987;10:512–6.
Tomiyama. Close relationship of abnormal glucose tolerance with endothelial dysfunction in hypertension. Hypertension 2000;36:245–9.
Cai H, DG Harrison. Endothelial dysfunction in cardiovascular diseases-the role of oxidant stress. Circ Res 2000;87:840–4.
Midaoui ELA, JD Champlain. Prevention of hypertension, insulin resistance and oxidative stress by lipoic acid. Hypertension 2002;39:303-7.
Rodrigo R. Relationship between oxidative stress and essential hypertension. Hypertens Res 2007;30:1159-67.
Stoclet JC. Vascular protection by dietary polyphenols. Eur J Pharmacol 2004;500:299-313.
Eman A. Identification of the chemical composition of the methanolic extracts of Salix tetrasperma Roxb using LC-MS and evaluation of its potential as an antioxidant agent. Pharm Chem 2015;7:168-77.
Modal Sumanta, Hechhu Ramana, Suresh P, Chhetree Rishi Raj. Studies on diuretic and laxative activity of the Salix tetrasperma roxburgh. Int Res J Pharm 2010;1:145-9.
