PROTECTIVE ROLE OF DAIDZEIN AGAINST CYCLOPHOSPHAMIDE INDUCED NEPHROTOXICITY IN EXPERIMENTAL RATS
DOI:
https://doi.org/10.22159/ijpps.2017v9i6.18516Keywords:
Daidzein, Cyclophosphamide, Serum creatinin, Glutathione, NephrotoxicityAbstract
Objective: To evaluate the protective effect of daidzein on cyclophosphamide (CPA) induced nephrotoxicity in experimental rats.
Methods: The Wistar rats of either sex were randomly divided into five equal groups: Group I (normal saline 1 ml p. o.), Group II (CPA 150 mg/mg i. p), Group III (Daidzein 20 mg/kg p. o.+CPA 150 mg/kg, i. p.), Group IV (Daidzein 40 mg/kg p. o.+CPA 150 mg/kg, i. p.) and Group V (Daidzein 40 mg/kg p. o., alone). Rats of all groups except Group I and Group V treated with CPA in a dosage of 150 mg/mg i. p in the last five days of treatment. Blood samples were collected on 11th day from each rat and subjected for the evaluation of serum markers such as Serum creatinin, serum urea and serum uric acid. Kidney of each rat was excised and subjected for antioxidant parameters evaluation such as malonyl dialdehyde (MDA), glutathione (GSH), superoxide dismutase (SOD), catalase and histopathological study.
Results: Daidzein explored a significant (P<0.001) role in CPA-induced nephrotoxicity by suppressing serum creatinine, serum urea and serum uric acid. Daidzein also demonstrated significant (P<0.001) protection against CPA-induced nephrotoxicity by decreasing MDA level and by elevating the GSH, SOD, catalase at different doses.
Conclusions: The obtained results of present study revealed that daidzein attenuates the CPA-induced nephrotoxicity by antioxidant defence action in rats.
Downloads
References
Ahmed IA, Sohair RF, Fathi MA, Sara ME. Renoprotective effect of Mangifera indica polysaccharides and silymarin against cyclophosphamide toxicity in rats. J Basic Appl Zool 2015;72:154–62.
Deepti C, Harmeet SR, Vibha S, Ritu M. Chemotherapy-induced adverse drug reactions in oncology patients: a prospective observational survey. Indian J Med Paediatr Oncol 2016;37:42-6.
Mbong AM, Djiokeng PG, Ntentie FR, Dimodi H, Ngondi JL, Oben EJ. Protective effect of hydroethanolic extracts of Solanum scabrum and Cola verticillata against cyclophosphamide induced toxicity in female rats. J Food Res 2014;3:18-30.
Haque R, Hafeez B, Parvez S, Pandey S, Sayeed I, Ali M, et al. Aqueous extract of walnut (Juglans regia L.) protects mice against cyclophosphamide-induced biochemical toxicity. Human Exp Toxicol 2003;22:473-80.
Hany AO, Wafaa RM, Hany HA, El-Shaimaa AA. Tangeretin alleviates cisplatin-induced acute hepatic injury in rats: targeting MAPKs and apoptosis. J Pone 2016;1-18. https://doi.org/10.1371/journal.pone.0151649
Adem BA. Neuroprotective effects of daidzein on focal cerebral ischemia injury in rats. Neural Regen Res 2015;10:146-52.
Yan-Bin Y, Ai-Ling C, Wei L, Shu-Yu Z, Juan L, Jian-Hua G, et al. Daidzein and genistein fail to improve glycemic control and insulin sensitivity in Chinese women with impaired glucose regulation: a double-blind, randomised, placebo-controlled trial. Mol Nutr Food Res 2015;59:240–9.
Jun MG, Bing XX, De JD, Qiong L, Hong HM. Effects of daidzein on estrogen receptor-positive and negative pancreatic cancer cells in vitro. World J Gastroenterol 2004;10:860-3.
Sanjiv K, Jagadish VK. Effect of daidzein on cisplatin-induced hemotoxicity and hepatotoxicity in experimental rats. Indian J Pharm 2017;49:1-6.
Kale MS, Laddha KS. Isolation, Characterization and quantification of isoflavone in Momordica dioica Roxb. Ex wild (Cucurbitaceae) fruits. Int J Appl Res Nat Prod 2013;5:28-36.
Ellmann GL. Tissue sulfhydryl groups. Arch Biochem Biophys 1959;82:70-7.
Ohkawa H, Ohishi N, Yagi K. Assay for lipid peroxidases in animal tissues by thiobarbituric acid reaction. Anal Biochem 1979;95:351–8.
Aebi H. Catalase. In: HU Bergmeyer. Ed. Methods in Enzymatic Analysis. Vol. 3. Academic Press: New York; 1983. p. 276–86.
Kakkar PS, Das B, Viswanathan PNA. Modified spectrophotometric assay of superoxide dismutase. Indian J Biochem Biophys 1984;21:130–2.
Bancroft JD, Gamble M. Theory and practice of histological techniques. 5th ed., Churchill Livingstone Pub., Edinburgh/New York/london/Philadelphia; 2002. p. 125-38, 172-5, 184-93, 593-620.
Eman S, Wagdi FE, Rania RA. Tranilast ameliorates cyclophosphamide-induced lung injury and nephrotoxicity. Can J Physiol Pharmacol 2015;94:1-12.
Sangita S, Swarn L, Kavindra NT. The potential protective role of aqueous extract of Aegle marmelos against cyclo-phosphamide induced nephrotoxicity in mice. J Exp Zool 2015;18:75-82.
Mojtaba K, Mehdi G, Mohammad JK, Javad B, Hossein F, Nima B, et al. Protective effects of the hydroalcoholic extract of Capparis Spinosa L. against cyclophosphamide-induced nephrotoxicity in mice. J Nat Pharm Prod 2016;1-7. Doi:10.17795/jjnpp-37240
Agarwal AD. Pharmacological activities of Flavonoids–a review. Int J Pharm Nanotech 2011;4:1394-8.
Kadon KH, Jun Ren. Phytoestrogenic isoflavones daidzein and genistein reduce glucose toxicity induced cardiac contractile dysfunction in ventricular myocytes. Endo Res 2004;30:215-23.
Rasoul E, Babak H, Hamze M, Hadi S. Vitamin E ameliorates cyclophosphamide-induced nephrotoxicity. Life Sci J 2013; 10:308-13.
Gultekin F, Delibas N, Yasar S, Kilinc I. In vivo changes in antioxidant systems and protective role of melatonin and a combination of vitamin C and vitamin E on oxidative damage in erythrocytes induced by chlopyrifos-ethyl in rats. Arch Toxicol 2001;75:88-96.
Max CY, Bernard P, Roy S, Onni N, Heidi K, Seppo P, et al. The cytoprotective effect of α-tocopherol and daidzein against D-galactosamine–induced oxidative damage in the rat liver. Metabolism 2007;56:865-75.
Published
How to Cite
Issue
Section
