IMPACTS OF DIFFERENT CONCENTRATIONS OF AQUEOUS GREEN TEA EXTRACT ADMINISTERED DURING METHOTREXATE TREATMENT ON SOME SELECTED BLOOD INDICES IN RATS
Keywords:
Methotrexate, Aqueous Green tea extract, Blood indices, RatsAbstract
Objective: This study was designed to investigate the impacts of administering of different concentrations of Aqueous Green Tea Extract (AGTE) (0.625, 1.25 or 2.5%) during Methotrexate (MTX) treatment, and continued for 5 days, on blood indices in rats.
Methods: Six groups of white Albino rats were utilized: Â Group I- (Control, received water); Group II- [single i. p. injection (20 mg/kg) of MTX], Group III- [1.25% concentration of AGTE alone for 5 days], and Groups IV, V and VI- [rats were administered, respectively AGTE (0.625, 1.25 or 2.5%), during MTX treatment, and continued for 5 days].
Results: The results showed that MTX alone induced significant decreases in Hb, HCT, MCHC, MCH, RBCs, and total WBCs; while, it increased platelets count. Administration of different concentrations of AGTE during MTX produced significant decrease in total WBCs and platelets; Â while significant increase in Hb, HCT, MCHC, MCH; Â meanwhile, significant increase, or decrease, respectively in MCV (depending on the concentration of AGTE); and a significant increase in RBCs only was seen at 2.5% AGTE plus MTX), compared to MTX-treated animals.
Conclusion: Marked alterations in blood indices were obtained from this study when AGTE was administered during MTX treatment. So this study recommends taking into account such administration when MTX is therapeutically utilized.
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