ANTIBACTERIAL ACTIVITY OF FLAVONOID FROM KEPEL (STELECHOCARPUS BURAHOL) LEAVES AGAINST STAPHYLOCOCCUS EPIDERMIDIS
Keywords:Fractionation, Flavonoid, Stelechocarpus burahol, Antibacterial, Staphylococcus epidermidis
Objective: The objective of this research was to separate flavonoids of kepel (Stelechocarpus burahol) leaves from methanol extract as antibacterial agent against Staphylococcus epidermidis that have a role in body odor.
Methods: The methanol extracts of kepel was fractionated in methanol: water (7:3), n-hexane and chloroform consecutively. Methanol: water (7:3) extract with the highest flavonoids content, fractioned by silica gel column chromatography (isocratic elution, with n-buthanol: methanol: acetic acid (1:8:1) as eluent) to produce 7 fractions. All fractions were tested for antibacterial activity with a microdilution method. The most active fractions was determined using UV-VIS spectrophotometer (Shimadzu, Japan) and FTIR (Brucker, Germany).
Results: Fraction V was the most active fraction with minimum inhibitory concentration (MIC) 0.06 mg/ml and minimum bactericidal concentration (MBC) of 0.50 mg/ml. Fraction V was further separated by preparative thin layer chromatography (TLC) and gave three fractions. Fraction V3 was the most active fraction with MIC 1.00 mg/ml and MBC of 2.00 mg/ml. Identification of fraction V3 based on assessments on ultraviolet-visible and infrared spectrum showed the maximum wavelength at 327 nm. These results indicate a transition Ï€ â†’ Ï€ * and n â†’ Ï€ * generated from the chromophore conjugated C = C and C = O. Based on the allegations of the functional group obtained, then alleged that in Fraction V3 containing flavones compounds.Conclusion: These results suggest that flavonoid of S. burahol leaves extracts is potential as antibacterial agents against S. epidermidisand therefore justifies their usage in traditional medicine for the treatment of body odor.
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