A METHOD FOR DETERMINING 1,4-BENZOTHIAZINE DERIVATIVES IN RAT PLASMA BY HPLC AND ITS APPLICATION TO A PHARMACOKINETIC STUDY

Amit Rai; Vinit Raj; Ashok K. Singh; Amit K. Keshari; Sudipta Saha

doi:10.22159/ijpps.2017v9i12.19339

Authors

Amit Rai Department of Pharmaceutical Sciences, Babasaheb Bhimrao Ambedkar University, Vidya Vihar, Rae bareli Road, Lucknow 226025, India
Vinit Raj Department of Pharmaceutical Sciences, Babasaheb Bhimrao Ambedkar University, Vidya Vihar, Rae bareli Road, Lucknow 226025, India
Ashok K. Singh Department of Pharmaceutical Sciences, Babasaheb Bhimrao Ambedkar University, Vidya Vihar, Rae bareli Road, Lucknow 226025, India
Amit K. Keshari Department of Pharmaceutical Sciences, Babasaheb Bhimrao Ambedkar University, Vidya Vihar, Rae bareli Road, Lucknow 226025, India
Sudipta Saha Department of Pharmaceutical Sciences, Babasaheb Bhimrao Ambedkar University, Vidya Vihar, Rae bareli Road, Lucknow 226025, India

DOI:

https://doi.org/10.22159/ijpps.2017v9i12.19339

Keywords:

1, 4-benzothiazine derivatives (AR13 and AR15), Pharmacokinetics, HPLC, Rat plasma

Abstract

Objective: The objective of the study was to develop, optimize and validate of a new reverse-phase high-performance liquid chromatography (RP-HPLC) method for the determining 1,4-benzothiazine derivatives (AR13 and AR15) in a biological sample of rat plasma. The 1,4-benzothiazine derivatives are produced by the synthetic reactions.

Methods: RP-HPLC separation was performed using an ODS-2 Hypersil column with gradient elution mobile phase consisting of water-acetonitrile for AR13 and AR15 (1:9 v/v, 3:7 v/v) at room temperature 1 ml/min flow rate, and interfaced with photodiode array detector (PDA) detector, 233 nm, 235 nm respectively.

Results: A linear response was obtained between (range from 0.100-10.00 mg/ml) AR13 and (range from 0.096â€“9.88 mg/ml) AR15 with correlation coefficient 0.999 and 0.998. The linearity range of both AR13 and AR15 was 101.65Â±1.5 and 98.78Â±1.7.

Conclusion: It was concluded that the method was simple, accurate, sensitive, accurate and reproducible and has been successfully applied to the pharmacokinetic study of AR13 and AR15 in rat plasma.

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