EFFECT OF COSTUS PICTUS D. DON ON CARBOHYDRATE HYDROLYZING ENZYMES
Keywords:
Alpha-amylase, Alpha-glucosidase, Costus pictus, AntidiabeticAbstract
Objective: Costus pictus D. Don is a potent anti-diabetic plant used in folk as well as Indian traditional medicine. In the present study, 4 extracts namely, Fresh-aqueous, Fresh-hydroalcoholic, Dry-Aqueous and Dry-hydroalcoholic prepared from fresh as well as dried leaves of C. pictus were evaluated for their effect on alpha-amylase and alpha-glucosidase enzymes using In vitro assays.
Methods: Alpha-Amylase inhibitory activity of C. pictus extracts was evaluated using Porcine pancreatic alpha-amylase (PPA) with starch as a substrate, whereas, alpha-glucosidase inhibitory activity was evaluated using p-nitrophenyl-α-D-Glucopyranoside (PNPG) as a substrate.
Results: Only Fresh-aqueous extract of C. pictus revealed potent alpha-amylase inhibitory (IC50= 9.90mg/ml) as well as alpha-glucosidase inhibitory (IC50= 2.51mg/ml) activities. In general, it exhibited strong inhibitory effect on alpha-glucosidase than alpha amylase.
Conclusion: Present study does seem to justify usage of fresh leaves of C. pictus as a munching dilatory supplement for treatment of diabetes as Fresh-aqueous extract exhibited potent inhibitory effect on carbohydrate hydrolyzing enzymes.
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