CHEMICAL MODIFICATION, CHARACTERIZATION AND EVALUATION OF MUCOADHESIVE POTENTIALITY OF ASSAM BORA RICE STARCH
DOI:
https://doi.org/10.22159/ijpps.2017v9i9.20108Keywords:
Assam bora rice starch, Ex-vivo mucoadhesion, Modified starch, In vitro drug releaseAbstract
Objective: The objective of the present study was to chemical modification, characterization and evaluation of mucoadhesive potentiality of Assam bora rice starch as potential excipients in the sustained release drug delivery system.
Methods: The starch was isolated from Assam bora rice and esterified using thioglycolic acid and characterized by Fourier transform infrared spectroscopy (FT-IR), Differential scanning calorimetry (DSC) and Nuclear magnetic resonance (NMR). The 10% w/v gel formulation based on modified bora rice starch loaded with irinotecan (0.6%) was prepared and evaluated for various rheological properties, ex-vivo mucoadhesion using goat intestine and in vitro drug release study in phosphate buffer pH 6.8.
Results: The chemical modification was confirmed by FT-IR and NMR studies with the presence of the peak at 2626.74 cm-1 and a singlet at 2.51 respectively due to–SH group. Ex-vivo mucoadhesion studies showed 6.6 fold increases in mucoadhesion of the modified starch with compared to native starch (46.3±6.79g for native starch; 308.7±95.31g for modified starch). In vitro study showed 89.12±0.84 % of drug release after 6 h in phosphate buffer pH 6.8 and the release kinetics followed Non-Fickian diffusion.
Conclusion: The modified Assam bora rice starch enhanced a mucoadhesive property of the native starch and thus, can be explored in future as a potential excipient for the sustained release mucoadhesive drug delivery system.
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Bernkop-Schnürch A, Kast CE, Richter MF. Improvement in the mucoadhesive properties of alginate by the covalent attachment of cysteine. J Controlled Release 2001;71:277–85.
Vasir JK, Tambwekar K, Garg S. Bioadhesive microspheres as a control drug delivery system. Int J Pharm 2003;255:13-32.
Ahmed A, Bonner C, Desai TA. Bioadhesive micro devices with multiple reservoirs: a new platform for oral drug delivery. J Controlled Release 2002;81:291-306.
Bernkop-Schnürch A. Thiomers: a new generation of mucoadhesive polymers. Adv Drug Delivery Rev 2005;57:1569–82.
Gum JR, Hicks JW, Toribara J, Rothe NW, Lagace EM, Kim YS. The human MUC2 intestinal mucin has cysteine-rich subdomains located both upstream and downstream of its central repetitive region. J Biol Chem 1992;267:21375–83.
Bernkop-Schnürch A, Hornof M, Zoidl T. Thiolated polymers–thiomers: synthesis and in vitro evaluation of chitosan-2-imino-thiolane conjugates. Int J Pharm 2003;260:229–37.
Bernkop-Schnurch A, Steininger S. Synthesis and characterization of mucoadhesive thiolated polymers. Int J Pharm 2001;194:239–47.
Sachan KN, Bhattacharya A. Evaluation of Assam bora rice starch as a possible natural mucoadhesive polymer in the formulation of micro particulate control drug delivery systems. J Assam Sc Soc 2006;47:34-41.
Rajak P, Nath LK, Bhuyan B. Application of assam bora rice starch as a binder in the formulation of paracetamol tablets. Int J Pharm Pharm Sci 2014;6:118-20.
http://www.foodrepublic.com/2011/08/12/what-makes-sticky-rice-sticky. [Last accessed on 10 Apr 2017]
Sriamornsak P, Sungthongjeen S, Puttipipitkhachorn S. Use of pectin as a carrier for intragastric floating drug delivery: carbonate salt contained beads. Carbohyd Polym 2007;671:436–45.
Ahmed MZ, Akhter S, Ahmed I, Singh A, Anwar M, Shamim M, et al. In vitro and in vivo evaluation of Assam bora rice starch-based bioadhesive microsphere as a drug carrier for colon targeting. Expert Opin Drug Delivery 2011;9:1-9.
Putri KSS, Surini S, Anwar E. Pregelatinized cassava starch phthalate as a film-forming excipient for a transdermal film of ketoprofen. Asian J Pharm Clin Res 2013;6:62-6.
Liu H, Ramsden L, Corke H. Physical properties of cross-linked and acetylated normal and waxy rice starch. Starch-Starke 1999;51:249–52.
Li Y, Zhang H, Shoemaker FC, Xua Z, Zhud S, Zhonga F. Effect of dry heat treatment with xanthan on waxy rice starch. Carbohyd Polym 2013;92:1647–52.
Ackar D, Babic J, Subaric D, Kopjar M, Milicevic B. Isolation of starch from two wheat varieties and their modification with epichlorohydrin. Carbohyd Polym 2010;81:76–82.
Hasjim J, Li E, Dhital S. Milling of rice grains: Effects of starch/flour structures on gelatinization and pasting properties. Carbohyd Polym 2013;92:682–90.
Singh L, Sharma V, Sharma S. Preparation and characterization of modified starch isolated from amaranthus paniculatus linn. Int J Curr Pharm Res 2011;3:62-4.
Sharma R, Ahuja M. Thiolated pectin: synthesis, characterization and evaluation as a mucoadhesive polymer. Carbohyd Polym 2011;85:658–63.
Mahajan HS, Tyagi VK, Patil RR, Dusunge SB. Thiolated xyloglucan: synthesis, characterization and evaluation as mucoadhesive in situ gelling agent. Carbohyd Polym 2013;91:618–25.
Kaur H, Yadav S, Ahuja M, Dilbaghi N. Synthesis, characterization and evaluation of thiolated tamarind seed polysaccharide as a mucoadhesive polymer. Carbohyd Polym 2012;90:1543–9.
Martinac A, Filipovi c-Grcic J, Voinovich D, Perissutti B, Franceschinis E. Development and bioadhesive properties of chitosan–ethylcellulose microspheres for nasal delivery. Int J Pharm 2005;291:69–77.
Shi R, Zhu A, Chen D. In vitro degradation of starch/PVA films and biocompatibility evaluation. J Appl Polym Sci 2010;115:346–57.
Suggs LJ, Krishan RS, Garcia CA, Peter SJ, Anderson JM. In vitro and in vivo degradation of poly (propylene fumarate-co-ethylene glycol) hydrogel. J Biomed Mater Res 1998;42:312–20.
Ahmed AB, Nath LK. Design and development of controlled release floating matrix tablet of Nicorandil using hydrophilic cellulose and pH-independent acrylic polymer: in vitro and in vivo evaluations. Expert Opin Drug Delivery 2016;13:315-24.
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