ANTIOXIDANT EFFECT OF POLYHERBAL FORMULATION IN ISOPRENALINE HYDROCHLORIDE INDUCED MYOCARDIAL INFARCTION IN RATS
DOI:
https://doi.org/10.22159/ijpps.2017v9i10.20310Keywords:
Myocardial infarction, Polyherbal formulation, Isoprenaline hydrochloride, Oxidative stress, Free radicalsAbstract
Objective: Myocardial infarction (MI) continues to be a major public health problem in the world. The prepared polyherbal formulation (PHF) exhibit cardioprotective effects by several mechanisms. The objective of the research was to evaluate the antioxidant effect of a polyherbal formulation in isoprenaline hydrochloride (ISO) induced myocardial infarction in rats.
Methods: Oxidative stress and myocardial infarction was induced by administration of isoprenaline hydrochloride (25 mg/kg) for 2 d. The degree of protection was determined by measuring the levels of lipid peroxidation (LPO), enzymatic antioxidants (superoxide dismutase-SOD, Catalase, Glutathione peroxidase) and non-enzymatic antioxidants (Vitamin C, E and reduced glutathione-GSH).
Results: Two different doses (250 mg and 500 mg/kg body wt) of the formulation was used to prove the cardioprotective effect. Pretreatment with polyherbal formulation significantly (P<0.05) lowered LPO and elevated the enzymatic and non-enzymatic antioxidants in heart homogenate.
Conclusion: The polyherbal formulation exhibited cardioprotective activity, which could be attributed to its antioxidant activity.Â
Downloads
References
Farnsworth NR, Akerele O, Bingel AS, Soejarto DD, Guo ZG. Medicinal plants in therapy. Bull WHO 1985;63:965-81.
Halliwell B, Aruoma OI. DNA damage by oxygen derived species. Its mechanism and measurement in mammalian systems. FEBS Lett 1991;281:9–19.
Dhevi R, Elango V, Gayathri K. Cardioprotective and antioxidant effects of seeds of Spermacoce hispida linn., on isoproterenol induced myocardial infarction in rats. W J Pharm Pharm Sci 2014;3:1150-8.
Deepa P, N Kannappan. Comparative in vitro antioxidant studies of aqueous solution of formulated poly herbal formulation with marketed preparation. Scholar Res Lib Pharm Lett 2012;4:1515-17.
Rajalakshmy, Ramya Pydi S, Kavimani. Cardioprotective medicinal plants-a review. Int J Pharm Inven 2011;1:24-41.
Das S, Vasisht S, Senhlata C, Das N, Srivastava LM. Correlation between total antioxidant status and lipid peroxidation in hypercholesterolemia. Curr Sci 2000;78:486-7.
Sinha AK. Colorimetric assay of catalase. Anal Biochem 1972;47:389-94.
Ellman GL, Fiches FT. Quantitative determination of peptides by sulfhydryl groups. Arch Biochem Biophy 1959;82:70-2.
Omaye DW, Emery G, Maynard JE. Selected methods for the determination of ascorbic acid in animal cells, tissues, and fluids. Methods Enzymol 1979;62:3-11.
Varley H, Gowenlock AH, Bell M. Practical clinical biochemistry. William Heineman Medical Books Ltd: London; 1981;2:222.
Reitman S, Frankel S. A colorimetric method for determination serum glutamic oxaloacetic and glutamic pyruvic transaminases. Am J Clin Pathol 1957;28:56-63.
Moron MS, Defierre JW, Mannervak D. Levels of glutathione, glutathione reductase and glutathione-S-transferase activities in rat lung and liver. Biochem Biophys Acta 1979;582:67-8.
Sun DQ, Nguyen N, Degrado TR. Ischemia induces translocation of the insulin responsive glucose transporter GLUT-4 to the plasma membrane of the cardiomyocytes. Cirt Res 1994;89:793.
Jayalakshmi R, Niranjali Devaraj S. Cardioprotective effect of crataegus on isoproterenol induced myocardial infarction in rats. J Pharm Pharmacol 2004;56:921-6.
Nnodim John Kennedy, Dike Ndudin Joy, Elendu Humphery Ndubueze, Nwagbaraocha Melvina, Egbuobi Richard, Onyeze vitus. Antioxidant and cardioprotective effect of cocnut water against doxirubicin induced cardiomyopathy. J Krishna Inst Med Sci Univ 2013;2:37-41.
Chabukswar AR, Kuchekar BS, Jagdale SC, Lokhande PD, Raut CG. Cardioprotective activity of Inula racemosa. Int J Chem Sci 2010;8:1545-52.
Published
How to Cite
Issue
Section
