• Ayman A. Gouda Chemistry Department, Faculty of Sciences, Zagazig University, Zagazig, 44519, Egypt, Faculty of Public Health and Informatics, Umm AL-Qura University, Makkah, Kingdom of Saudi Arabia
  • Atiat M. Barakat Chemistry Department, Faculty of Sciences, Zagazig University, Zagazig, 44519, Egypt, Department of Chemistry, Faculty of Science, Taibah University, Al-Madinah Al-Munawarah, Kingdom of Saudi Arabia.


Desloratadine, Ebastine, Charge transfer complexes, Spectrophotometry, Pharmaceutical formulations


Objective: Simple, accurate and precise spectrophotometric methods have been developed for the determination of two antihistaminic drugs (desloratadine (DES) and ebastine (EBS)) in pure forms and pharmaceutical formulations.

Methods: The proposed methods were based on the charge transfer complexation reaction of both drugs as ‘n' electron donor with chloranilic acid (p-CLA) or 2, 3-Dichloro-5,6-dicyano-p-benzoquinone (DDQ) as π acceptors to give highly coloured complex species. The coloured products were quantitated spectrophotometrically at 459 and 460 nm using DDQ and at 532 and 533 nm using p-CLA for DES and EBS, respectively. Optimization of the different experimental conditions were studied.

Results: Beer's law was obeyed in the concentration ranges of 5.0-120 and 10-180 mg mL-1 with good correlation coefficients were ≥ 0.9995 and 0.9992 and a relative standard deviation (R.S.D.) of ≤ 0.98 and 1.24% using DDQ and p-CLA methods, respectively. The molar absorptivity, Sandell's sensitivity, detection and quantification limits were also calculated. The developed methods were successfully applied for determination of the studied drugs in pharmaceutical formulations with good accuracy and precision and without interferences from common additives by applying the standard addition technique.

Conclusion: The developed methods have been validated statistically for their accuracy, precision, sensitivity, selectivity, robustness and ruggedness as per ICH guidelines and the results compared favorably with those obtained using the reported methods.


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How to Cite

Gouda, A. A., and A. M. Barakat. “CHARGE TRANSFER SPECTROPHOTOMETRIC METHODS FOR THE DETERMINATION OF TWO ANTIHISTAMINIC DRUGS IN PHARMACEUTICAL FORMULATIONS”. International Journal of Pharmacy and Pharmaceutical Sciences, vol. 6, no. 7, July 2014, pp. 334-41,



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