EXPLORATION OF GOITROGENIC/ANTITHYROIDAL POTENTIALITY OF BAMBOO-SHOOTS IN RELATION TO THIOUREA
DOI:
https://doi.org/10.22159/ijpps.2017v9i10.20332Keywords:
Dietarygoitrogen, Thyroid, Thiourea, Bamboo-shoots, Thyroid peroxidiseAbstract
Objective: Natural goitrogens present in plant foods or antithyroidal drugs both alter the morphology and normal functional status of the thyroid gland interfering the synthesis and secretion of thyroid hormones. In this study, the goitrogenic potential of bamboo shoots (BS) containing natural goitrogens has been compared against a potent synthetic antithyroidal drug thiourea.
Methods: BS was fed to 6 rats by 1/3rdreplacement of 180grams (g) of their food i.e.60g of BS containing 35g of goitrogens of cyanogenic origin such that each rat ideally consumed 6 mg/100g of body weight per day and thiourea at a near equal dose of 6 mg/100g of body weight per day was force fed to another 6 rats for a period of 45 d. Changes in food consumption pattern, serum toxicity marker SGOT and SGPT, morphological changes like thyroid gland weight and histology and functional aspects like TPO activity, Na+-K+ATPase activity, urinary iodine excretion and serum T3,T4 and TSH levels induced by this natural and synthetic agent respectively were investigated. Results analyzed by ANOVA followed by post hoc tests.
Results: Food consumption pattern was similar in all groups while serum toxicity markers SGOT, SGPT showed a non-significant increase in experimental groups. Increase in body and thyroid gland weight as compared to control was seen on treatment with both antithyroidal agents, a greater percentage of increase was seen in thiourea treated group. TPO activity, Na+-K+ATPase activity, serum T3 and T4levels were decreased significantly in both treated groups as compared to normal, with maximum inhibition in the thiourea treated group. Histological plates of BS treated thyroid revealed hypertrophy and thyroid follicular cell disruption with microcytic infiltration. Thiourea treated gland histological plate revealed complete abolition of normal histoarchitecture with marked changes in colloidal area and hyperplasia with folding and papillae on the epithelium.
Conclusion: In overall, the data indicate that though the goitrogenic potentiality of BS cannot be considered having similar efficacy to that of thiourea but as a food entity by itself, it has potent antithyroidal activity even in presence of adequate iodine intake.
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Manna D, Roy G, Mugesh G. Antithyroid drugs and their analogues: synthesis, structure, and mechanism of action. Acc Chem Res 2013;46:2706-15.
Criteria Group for Occupational Standards. Scientific basis for Swedish occupational standards XXII. Consensus report for Diisocyanate (TDI), Diphenylmethane Diisocyanate (MDI), Hexamethylene Diisocyanate (HDI). Arbeteoch Halsa 2001;20:60-88.
Hosoya T. Effect of various reagents including antithyroid compounds upon the activity of thyroid peroxidase. J Biochem 1963;53:381-8.
Morgans M. Toxic effects of thiourea, thiouracil, methyl thiouracil, and aminothiazole in thyrotoxicosis. Lancet 1947;249:519-21.
Chandra AK, Singh L, Ghosh S, Pearce E. Role of bamboo-shoot in the pathogenesis of endemic goiter in Manipur, North East India. Endocr Pract 2012;19:36-45.
Chandra AK, Mukhopadhyay S, Lahari D, Tripathy S. Goitrogenic content of Indian cyanogenic plant foods and their in vitro anti-thyroidal activity. Indian J Med Res 2004;119:180.
Chakraborty A, Mandal J, Mondal C, Sinha S, Chandra AK. Effect of excess iodine on oxidative stress markers, steroidogenic—enzyme activities, testicular morphology, and functions in adult male rats. 2015. Biol Trace Elem Res 2016;172:380–94.
Chandra AK, Ghosh D, Mukhopadhyay S, Tripathy S. Effect of bamboo shootBambusaarundinacea (Retz.) Wild on thyroid status under conditions of varying iodine intake in rats. Indian J Exp Biol 2004;42:781-6.
Material Safety Datasheet on thiourea. Available from: www.clayton.edu/portals/690/chemistry/inventory/MSDS%20Thiourea.pdf. [Last accessed on 19 Apr 2017]
Cooper DS. Antithyroid drugs. N Engl J Med 2005;352:905-17.
Alexander NM. Assay procedure for thyroid peroxidase. Anal Biochem 1962;4:341-5.
Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the Folin phenol reagent. J Biol Chem 1951;193:265-75.
Skou JC, Esmann M. Preparation of membrane Na+, K+-ATPase from rectal glands of Squalusacanthias. Methods Enzymol 1988;156:43-6.
Baginski ES, FoaPP, Zak B. Determination of phosphate: a study of labile organic phosphate interference. Clin Chim Acta 1967;15:155-8.
Ohashi T, Yamaki M, Pandav CS, Karmarkar MG, Irie M. Simple microplate method for determination of urinary iodine. Clin Chem 2000;46:529-36.
Stats V. Website for Statistical Computation; 2015.
Michajlovskij N, Sedlak J, Kostekova O. Content of naturally occurring goitrogens in boiled plants of the brassica family. Endocrinol Exp 1969;4:51-62.
Emiliano AB, Governale L, Parks M, Cooper DS. Shifts in propylthiouracil and methimazole prescribing practices: antithyroid drug use in the United States from 1991 to 2008. J Clin Endocrinol Metab 2010;95:2227-33.
Tresina PS, Sornalakshmi V, Paulpriya K, Mohan VR, Arumugasamy K. Hepatoprotective effect of Hedyotisleschenaultiana dc, ethanol extract in ccl4 induced hepatotoxicity in wistar rats. Asian J Pharm Clin Res 2014;7:287-90.
Ray G, Fisher D, Chopra I. Relation of thyroid hormones to body-weight. Lancet 1976;307:1206-8.
Mackenzie CG, MacKenzie JB. Effect of sulfonamides and thioureas on the thyroid gland and basal metabolism. Endocrinology 1943;32:185-209.
Chandra AK, Ghosh D, Mukhopadhyay S, Tripathy S. Effect of cassava (Manihotesculentacrentz) on thyroid status under conditions of varying iodine intake in rats. Afr J Tradit Complementary Altern Med 2006;3:87-99.
Graham SL, Davis KJ, Hansen WH, Graham CH. Effects of prolonged ethylene thiourea ingestion on the thyroid of the rat. Food Cosmet Toxicol 1975;13:493-9.
Chandra AK, Mukhopadhyay S, Ghosh D, Tripathy S. Effect of radish (Raphanussativus Linn.) on thyroid status under conditions of varying iodine intake in rats. Indian J Exp Biol 2006;44:653.
Faber J, Kirkegaard C, Rasmussen B, Westh H, Busch-Sørensen M, Jensen IW. Pituitary-thyroid axis in critical illness. J Clin Endocrinol Metab 1987;65:315-20.
Ruf J, Carayon P. Structural and functional aspects of thyroid peroxidase. Arch Biochem Biophys 2006;445:269-77.
Doerge DR, Takazawa RS. Mechanism of thyroid peroxidase inhibition by ethylene thiourea. Chem Res Toxicol 1990;3:98-101.
Ewart HS, Klip Amifu. Hormonal regulation of the Na (+)-K (+)-ATPase: mechanisms underlying rapid and sustained changes in pump activity. Am J Physiol Cell Physiol 1995;269:295-311.
Raby C, Lagorce JF, Jambut-Absil AC, Buxeraud J, Catanzano G. The mechanism of action of synthetic antithyroid drugs: iodine complexation during oxidation of iodide. Endocrinology 1990;126:1683-91.
Mondal C, Sinha S, Chakraborty A, Chandra AK. Studies on Goitrogenic/Antithyroidal potentiality of thiocyanate, catechin and after concomitant exposure of thiocyanate-catechin. Int J Pharm Clin Res 2016;8:108-16.
Chakraborty A, Mondal C, Sinha S, Mandal J, Chandra AK. Amiodarone-induced oxidative stress in stress-vulnerable organs of adult male rats. Asian J Pharm Clin Res 2014;4:177-83.
Chandra AK. Iodine, thiocyanate and the thyroid. Biochem Pharmacol 2015;4:3.
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