ANTIDIABETIC ACTIVITY AND TOXICOLOGICAL EVALUATION OF THE METHANOL-DICHLOROMETHANE ROOT BARK EXTRACT OF NAUCLEA DIDERRICHII (DE WILD) MERR
DOI:
https://doi.org/10.22159/ijpps.2017v9i9.20556Keywords:
Antidiabetic, Toxicity study, Biochemical and hematological parameters, Nauclea diderrichiiAbstract
Objective: In southeastern Nigeria, Nauclea diderrichii (De Wild) Merr is used in the treatment of a wide range of ailments including diabetes mellitus (DM). This study evaluates the antidiabetic activity and toxicological profile of the methanol-dichloromethane root bark extract of N. diderrichii in normoglycemic and alloxan-induced diabetic models.
Methods: Dried root barks of N. diderrichii were extracted using methanol and dichloromethane (1:1) to obtain N. diderrichii extract (NDE). The acute and sub-chronic toxicity tests were performed using standard procedures. The effect on alanine aminotransferase (ALT) aspartate aminotransferase (AST), alkaline phosphatase (ALP), total protein (TP), packed cell volume (PCV), hemoglobin concentration (HB) and total white blood cell (TWBC) count was determined in the rats after treatment. In order to elucidate its antidiabetic mode of action, an oral glucose tolerance test (OGTT) was performed using glucose (2 g/kg) as substrate and alloxan (100 mg/kg; i. v.) induced diabetic model. Glibenclamide (GLI 0.2 mg/kg) was used as the reference standard drug.
Results: The results indicated that the LD50 of the extract is>5000 mg/kg. ALT, AST, ALP PCV, HB and TWBC were insignificantly (p>0.05) different compared with the control. No significant changes were observed in the organ weights compared with the control. In the acute and prolonged antidiabetic study, NDE (100, 200 and 400 mg/kg) significantly reduced the blood glucose level (BGL) by 14.66, 18.9, 25.80% and 75.11, 80.24, 83.74% respectively. In comparison, GLI, when administered, reduced BGL by 38.18 and 92.86% respectively.
Conclusion: N. diderrichii possesses antidiabetic activity with good toxicological profile.Downloads
References
Komalavalli T, Packia LM, Muthukumarasamy S, Mohan VR. Antidiabetic and antihyperlipidemic activity of Sonerila tinnevelliensis fischer whole plant in alloxan induced diabetic rats. Int J Curr Pharm Res 2015;7:53-7.
Malini P, Kanchana G, Rajadurai M. Antidiabetic efficacy of ellagic acid in streptozotocin-induced diabetes mellitus in albino Wistar rats. Asian J Pharm Clin Res 2011;4:124Â8.
Osigwe CC, Akah PA, Nworu CS, Okoye TC, Tchimene MK. Antihyperglycemic studies on the leaf extract and active fractions of Newbouldia laevis (Bignoniaceae). Pharmacol Pharm 2015;6:518-32.
Ezuruike UF, Prieto JM. The use of plants in the traditional management of diabetes in Nigeria: pharmacological and toxicological considerations. J Ethnopharmacol 2014;155:857-924.
Lamidi M, Ollivier E, Gasquet M, Faure R, Nze-Ekekang L, Balansard G. Structural and antimalarial studies of saponins from Nauclea diderrichii bark. Adv Exp Med Biol 1996;404:383-99.
Mustofa VA, Benoit-Vical F, Pelissier Y, Kone-Bamba D, Mallie M. Antiplasmodial activity of plant extracts used in West African traditional medicine. J Ethnopharmacol 2000;73:145-51.
Di Giorgio C, Lamidi M, Delmas F, Balansard G, Ollivier E. Antileishmanial activity of quinovic acid glycosides and cadambine acid isolated from Nauclea diderrichii. Planta Med 2006;72:1396-402.
Nwodo NJ, Agbo MO. Antitrypanosomal effects of methanolic extracts of Nauclea diderrichii (Merr) and Spathodea campanulata stem bark. J Pharm Allied Sci 2010;7:1219-27.
Orwac C, Mutua A, Kindt R, Jamnadass R, Simons A. Agroforestree database: a tree reference and selection guild version 4.0; 2009. Available from: http:// www. worldagroforestry.org/af/treedbl. [Last accessed on 06 Apr 2017]
Lorke D. A new approach to practical acute toxicity testing. Arch Toxicol 1983;54:272-89.
Reitman S, Frankel SA. Colourimetric method of determination of serum glutamicoxaloacetic and glutamic pyruvic transaminase. Am J Clin Pathol 1957;28:56-63.
Rec GSCC. Determination of alkaline phosphatase. J Clin Chem Clin Biochem 1972;10:281-91.
Cheesbrough M. Medical laboratory manual for tropical countries. 2nd ed. ELSB: Cambridge; 1991. p. 508-11.
Ramnik S. Methods and interpretations in medical laboratory technology. 4th ed. India: Medical Publishers Ltd; 1994. p. 184-99.
Schalm OW, Jain NC, Carol EJ. Textbook of veterinary hematology. 2nd ed. Philadephia: Lea and Febiger; 1975. p. 192-250.
Fröde TS, Medeiros YS. Animal models to test drugs with potential antidiabetic activity. J Ethnopharmacol 2008;115:173-83.
Tunali S, Yanardag R. Effect of vanadyl sulfate on the status of lipid parameters and on stomach and spleen tissues of streptozotocin-induced diabetic rats. Pharmacol Res 2006;53:271-7.
Niranjan PS, Singh D, Prajapati K, Jain SK. Antidiabetic activity of ethanolic extract of Dalbergia sissoo L. leaves in alloxanÂinduced diabetic rats. Int J Curr Pharm Res 2010; 2:24-7.
Sujith K, Darwin RC, Roosewelt C. Antidiabetic activity of methanolic extract of Butea frondosa leaves with its mechanism of action. Asian J Pharm Res 2011;4:93-8.
Obi BC, Okoye TC, Joshua PE, Onyeto CA, Alumanah EO. Comparative study of the antidiabetic and biochemical effects of metformin, glibenclamide and repaglinide in alloxan-induced diabetic rats. Afr J Pharm Pharmacol 2015;9:1026-36.
Spasov AA, Maxeiner MP, Bulanov AF. Antidiabetic properties of Gymnema sylvestre. Pharm Chem J 2008;42:626-9.
Akah PA, Uzodinma SU, Okolo CE. Antidiabetic activity of aqueous and methanol extract and fractions of Gongronema latifolium (Aselepidaceae) leaves in alloxan-induced diabetic rats. J Appl Pharm Sci 2011;1:99-102.
Omoniwa BP, Luka CD. Antidiabetic and toxicity evaluation of aqueous stem extract of Ficus asperifolia in normal and alloxan-induced diabetic albino rats. Asian J Exp Biol Sci 2012;3:726-32.
Agnaniet H, Mbot EJ, Keita O, Fehrentz JA, Ankli A, Gallud A, et al. Antidiabetic potential of two medicinal plants used in Gabonese folk medicine. BMC Complementary Altern Med 2016;16:1-8.
Cazarolli LH, Zanatta L, Alberton EH, Figueiredo MS, Folador P, Damazio RG, et al. Flavonoids: cellular and molecular mechanism of action in glucose homeostasis. Mini Rev Med Chem 2008;8:1032-8.
Osadebe PO, Uzor PF, Omeje EO, Agbo MO, Obonga WO. Hypoglycemic activity of the extract and fractions of Anthocleista vogelii (Planch) stem bark. Trop J Pharm Res 2014;13:1437-43.
Ogbonnia SO, Mbaka GO, Nkemehule FE, Emordi JE, Okpagu NC, Ota DA. Acute and subchronic evaluation of aqueous extracts of Newbouldia laevis (Bignoniaceae) and Nauclea latifolia (Rubiaceae) roots used singly or in combination in Nigerian traditional medicines. Br J Pharmacol 2014;5:55-62.
Ramaiah SK. Preclinical safety assessment: current gaps, challenges and approaches in identifying translatable biomarkers of the drug induced liver. Clin Lab Med 2011;31:161-72.
Aguwa CN, Aguiyi JC. Liver disease. In: Aguwa CN. editor. Therapeutic basis of clinical pharmacyin the tropics. Enugu, Nigeria: SNAAP Press Ltd; 2004. p. 528-40.
Olson H, Betton G, Robinson D, Karluss T, Monro A, Kolaja G, et al. Concordance of the toxicity of pharmaceuticals in humans and in animals. Regul Toxicol Pharmacol 2000;32:56-67.
Mukinda JT, Syce JA. Acute and chronic toxicity of the aqueous extract of Artemisia afra in rodents. J Ethnopharmacol 2007;112:138-44.
Bello I, Bakkouri AS, Tabana YM, Al-Hindi B, Al-Mansoub MA, Mahmud R, et al. Acute and sub-acute toxicity evaluations of the methanolic extract of Alstonia scholaris stem bark. Med Sci 2016;4:1-14.
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