IMPACT OF ACTIVE COMPOUNDS ISOLATED FROM BANANA (MUSA SP. VAR. NANJANGUD RASABALE) FLOWER AND PSEUDOSTEM TOWARDS CYTOPROTECTIVE AND DNA PROTECTION ACTIVITIES

Ramith Ramu; Prithvi S. Shirahatti; Shrisha Naik Bajpe; Varsha Reddy S. V.; M. N. Nagendra Prasad

doi:10.22159/ijpps.2017v9i10.20560

Authors

Ramith Ramu Department of Biotechnology, Sri Dharmasthala Manjunatheshwara College of Post Graduate Centre, Ujire, Dakshina Kannada â€“ 574240, Karnataka, India
Prithvi S. Shirahatti Department of Biotechnology, Sri Dharmasthala Manjunatheshwara College of Post Graduate Centre, Ujire, Dakshina Kannada â€“ 574240, Karnataka, India
Shrisha Naik Bajpe Department of Studies in Biotechnology, University of Mysore, Manasagangotri, Mysore 570006, Karnataka, India
Varsha Reddy S. V. Department of Biotechnology, Sri Dharmasthala Manjunatheshwara College of Post Graduate Centre, Ujire, Dakshina Kannada â€“ 574240, Karnataka, India
M. N. Nagendra Prasad Department of Biotechnology, Sri Jayachamarajendra College of Engineering, JSS Institution Camp, Manasagangothri, Mysore â€“ 570 006, Karnataka, India

DOI:

https://doi.org/10.22159/ijpps.2017v9i10.20560

Keywords:

Umbelliferone, Lupeol, Stigmasterol, Nil

Abstract

Objective: The present study was designed to evaluate for cytoprotective and DNA protective properties of the compounds isolated from ethanol extract of banana flower (EF) and ethanol extract of banana pseudostem (EE).

Methods: The four active compounds viz., umbelliferone (C1) andlupeol (C2) from EF and stigmasterol (C3) and Î²-sitosterol (C4) from EE were isolated by activity-guided repeated fractionation through silica gel column chromatography. The isolated compounds were evaluated for cytoprotective on erythrocytes and pTZ57R/T plasmid DNA protection against hydroxyl radicals.

Results: The study revealed that the compounds (C1-C4) at a concentration of 1 mg/ml exhibited 90% protection on erythrocytes membrane oxidation and also protect the pTZ57R/T plasmid DNA damage induced by hydroxyl radicals.

Conclusion: These results endorse an insight for a strong chemical basis to the alleged beneï¬cial role of EF and EE in reducing oxidative stress conditions.

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