SYNTHESIS, CHARACTERIZATION AND ANTIBACTERIAL ACTIVITY OF ALUMINIUM OXIDE NANOPARTICLES
Keywords:Al2O3 nanoparticles, XRD, FTIR, SEM, EDX, Antibacterial activity and MIC
Objective: In the present study, synthesized alumina (Al2O3) nanoparticles were characterized and their antibacterial activity against gram positive and gram negative organisms were studied.
Methods: The synthesis was carried out by coprecipitation method using aluminium sulfate and NaOH as precursors. The synthesized aluminium oxide nanoparticles were characterized by using X-ray diffraction (XRD), Fourier transforms infrared spectroscopy (FT-IR) and scanning electron microscope (SEM) with Energy Dispersive X-ray Analysis (EDX) techniques. Besides, this study determines the antibacterial activity and minimum inhibitory concentration (MIC) of Al2O3 nanoparticles against gram-positive (Staphylococcus aureus and Streptococcus mutans) and gram-negative (E. coli and Proteus vulgaris) bacteria.
Results: The average crystallite size of Al2O3 nanoparticles was found to be 35 nm by X-ray diffraction. FT-IR spectrum exhibited the peaks at 615 and 636 were assigned to the aluminium oxide stretching. The EDX measurements indicated the presence of Al along with O peaks. It indicates the purity of the sample. The antimicrobial assay revealed that E. coli showed a maximum zone of inhibition (39 mm) at 50 mg/ml concentration of Al2O3 nanoparticles.
Conclusion: In conclusion, aluminium oxide is a good antibacterial agent against both gram positive and gram-negative organisms.
Ravi Chandrika K, Kiranmayi P, Ravikumar RVSSN. Synthesis, characterization and antibacterial activity of ZnO nanoparticles. Int J Pharm Sci 2012;4:336-8.
Warheit DB. Toxicological highlight how meaningful are the results of nanotoxicity studies in the absence of adequate material characterization. Toxicol Sci 2008;101:183â€“5.
Slavica Stankic, Sneha Suman, Francia Haque, Jasmina Vidic. Pure and multi metal oxide nanoparticles: synthesis, antibacterial and cytotoxic properties. J Nanobiotechnol 2016; 14:73.
Corr SA. Metal oxide nanoparticles. Nanoscience 2013;1:180â€“234.
Haddad PS, Seabra AB. Biomedical applications of magnetic nanoparticles. In: Martinez AI. editor. Iron oxides: structure, properties and applications. New York; Nova Science Publishers; 2012. p. 165â€“88.
Hench LL. Bioceramics: from concept to clinic. J Am Ceram Soc 1991;74:1487-510.
Travitzky N, Kumar P, Sandhage KH, Janssen R. Claussen N. Rapid synthesis of Al2O3 reinforced Fe-Cr-Ni composites. Mater Sci Eng A 2003;344:245-52.
Ganguly P, Poole Warren J. In situ measurement of reinforcement stress in an aluminium-alumina metal matrix composite under compressive loading. Mater Sci Eng 2003:352:46-54.
Goffeau A. Drug resistance: the fight against fungi. Nature 2008;452:541â€“2.
Ravi Chandrika K, Kiranmayi P. Ravikumar RVSSN. Role of zinc oxide nanoparticles enhancing the antibacterial activity of antibiotics. Asian J Pharm Clin Res 2012;4:97-9.
Ibrahim Mohamed Hamouda. Current perspectives of nanoparticles in medical and dental biomaterials. J Biomed Res 2012;26:143-51.
Sadiq MI, Chowdhury B, Chandrasekaran N, Mukherjee A. Antimicrobial sensitivity of Escherichia coli to alumina nanoparticles. Nanomed: Nanotech Biol Med 2009;5:282â€“6.
Neethumol Varghese, Manjusha Hariharan, Benny Cherian A, Sreenivasan PV, Jenish Paul, et al. PVA-Assisted synthesis and characterization of nano Î±â€“alumina. Int J Sci Res 2014;4:1-4.
Qi L, Xu Z, Jiang X, Hu C, Zou X. Preparation and antibacterial activity of chitosan nanoparticles. Carbohydr Res 2004;339:2693â€“700.
Ruparelia JP, Arup KC, Siddhartha P, Duttagupta, Suparna M. Strain specificity in antimicrobial activity of silver and copper nanoparticles. Acta Biomater 2008;4:707â€“16.
Vijayalakshmi R, Rajendran V. Synthesis and characterization of nano-TiO2 via different methods. Arch Appl Sci Res 2012;4:1183â€“90.
Dobrucka R, DÅ‚ugaszewska J. Biosynthesis and antibacterial activity of Zno nanoparticles using Trifolium pratense flower extract. Saudi J Biol Sci 2016;23:517-23.
Geoprincy G, Nagendhra N, Gandhi S, Renganathan. Novel antibacterial effects of alumina nanoparticles on Bacillus cereus and Bacillus subtilis in comparison with antibiotics. Int J Pharm Pharm Sci 2012;4:544-8.