SEARCH FOR NEW ANTILEISHMANIAL CHEMOTHERAPEUTICS
DOI:
https://doi.org/10.22159/ijpps.2018v10i1.20859Keywords:
Betulinic acid, Spikenard oil, Cypress oil, Curry leaf oil, GCMS, In vitro antileishmanial activityAbstract
Objective: The objective of this work was to screen a number of compounds for their antileishmanial efficacy and cytotoxicity profiling.
Methods: Curry leaf oil, cypress oil and spikenard oil were identified by gas chromatography-mass spectrometry (GC/MS) analysis. Betulinic acid, spikenard oil, cypress oil and curry leaf oil were evaluated for their in vitro antileishmanial activity against Leishmania donovani AG83 wild-type, sodium stibogluconate resistant (SSG-resistant), paromomycin (PMM-resistant) and GE1 field type strains on axenic and cellular amastigote model and compared the results with standard drugs used to treat leishmaniasis.
Results: Betulinic acid showed strong antileishmanial activity against wild-type (SI= 192.8), SSG-resistant (SI= 19.3) and GE1 strains (SI= 100), whereas cypress oil has produced highest antileishmanial activity against PMM-resistant strains (SI= 15.09) among all the tested drugs. The data obtained also revealed that cypress oil had the maximum CC50 value of 452.9 μl among all standard and tested drugs.
Conclusion: All tested drugs had antileishmanial property but among them, betulinic acid possess strong antileishmanial activity in case of both wild-type and drug-resistant leishmaniasis.
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References
Desjeux P. The increase in risk factors for the leishmaniasis Worldwide. Trans R Soc Trop Med Hyg 2001;95:239–43.
Ritting MG, Bogdan C. Leishmania host-cell interaction: complexities and alternative views. Parasitol Today 2000;16:292-7.
Santos DO, Countinho CER, Madeira MF. Leishmaniasis treatment-a challenge that remains: a review. Parasitol Res 2008;103:1-10.
Freitas Junior LH, Chatelain E, Kim HA, Siqueira Neto JL. Visceral leishmaniasis treatment: what do we have, what do we need and how to deliver it? Int J Parasitol: Drugs Drug Resist 2012;2:11-9.
Singh N, Kumar M, Singh RK. Leishmaniasis: current status of available drugs and new potential drug targets. Asian Pac J Trop Med 2012;5:485-97.
Croft SL, Sundar S, Fairlamb AH. Drug resistance in leishmaniasis. Clin Microbiol Rev 2006;19:111-26.
Banjara MR. Combination therapy for visceral leishmaniasis. Int J Infection Microbiol 2013;2:32-3.
Bakkali F, Averbeck S, Averbeck D, Idaomar M. Biological effects of essential oils–a review. Food Chem Toxicol 2008;46:446-75.
Alankar S. A review on peppermint oil. Asian J Pharm Clin Res 2009;2:27-33.
Rawat P, Khan MF, Kumar M, Tamarkar AK, Srivastava AK, Arya KR, et al. Constituents from fruits of Cupressus sempervirens. Fitoterapia 2010;81:162–6.
Mascolo N, Autore G, Capasso F, Menghini A, Fasulo MP. Biological screening of Italian medicinal plants for anti-inflammatory activity. Phytother Res 1987;1:28–31.
Selim SA, Adam ME, Hassan SM, Albalawi AR. Chemical composition, antimicrobial and antibiofilm activity of the essential oil and methanol extract of the Mediterranean cypress (Cupressus sempervirens L.). BMC Complementary Altern Med 2014;14:179.
Biswas AK, Chatli MK, Sahoo J. Antioxidant potential of curry (Murraya koenigii L.) and mint (Mentha spicata) leaf extracts and their effect on colour and oxidative stability of raw ground pork meat during refrigeration storage. Food Chem 2012;133:467-72.
Nagappan T, Ramasamy P, Wahid ME, Segaran TC, Vairappan CS. Biological activity of carbazole alkaloids and essential oil of Murraya koenigii against antibiotic resistant microbes and cancer cell lines. Molecules 2011;16:9651-64.
Kesari AN, Gupta RK, Watal G. Hypoglycemic effects of Murraya koenigii on normal and alloxan-diabetic rabbits. J Ethnopharmacol 2005;97:247-51.
Sharma P, Vidyasagar G, Bhandari A, Singh S, Bhadoriya U, Ghule S, et al. A pharmacological evaluation of antidiarrhoeal activity of leaves extract of Murraya koenigii in experimentally induced diarrhoea in rats. Asian Pac J Trop Dis 2012;2:230-3.
Sathaye S, Bagul Y, Gupta S, Kaur H, Redkar R. Hepatoprotective effects of aqueous leaf extract and crude isolates of Murraya koenigii against in vitro ethanol-induced hepatotoxicity model. Exp Toxicol Pathol 2011;63:587-91.
Nakamura S, Nakashima S, Oda Y, Yokota N, Fujimoto K, Matsumoto T, et al. Alkaloids from Sri Lankan curry-leaf (Murraya koenigii) display melanogenesis inhibitory activity: structures of karapinchamines A and B. Bioorg Med Chem 2013;21:1043-9.
Birari R, Javia V, Bhutani KK. Antiobesity and lipid lowering effects of Murraya koenigii (L.) Spreng leaves extracts and mahanimbine on high fat diet induced obese rats. Fitoterapia 2010;81:1129-33.
Takemoto H, Ito M, Shiraki T, Yagura T, Honda G. Sedative effects of vapor inhalation of agarwood oil and spikenard extract and identification of their active components. J Nat Med 2008;62:41-6.
Agnihotri S, Wakode S, Ali M. Chemical composition, antimicrobial and topical anti-inflammatory activity of Valeriana jatamansi Jones essential oil. J Essen Oil Bearing Plants 2011;14:417-22.
Mathew M, Subramanian S. In vitro screening for anti-cholinesterase and antioxidant activity of methanolic extracts of ayurvedic medicinal plants used for cognitive disorders. PLoS One 2014;9:e86804.
Razack S, Khanum F. Anxiolytic effects of Nardostachys jatamansi DC in mice. Ann Phytomed 2012;1:67-73.
Khan MB, Hoda MN, Ishrat T, Ahmad S, Khan MM, Ahmad A, et al. Neuroprotective efficacy of Nardostachys jatamansi and crocetin in conjunction with selenium in cognitive impairment. Neurol Sci 2012;33:1011-20.
Chaudhary S, Chandrashekar KS, Pai KS, Setty MM, Devkar RA, Reddy ND, et al. Evaluation of antioxidant and anticancer activity of extract and fractions of Nardostachys jatamansi DC in breast carcinoma. BMC Complementary Altern Med 2015;15:50.
Aleem MA, Asad BS, Mohammed T, Khan RA, Ahmed MF, Anjum A, et al. Antidiabetic activity of hydroalcoholic extracts of Nardostachys jatamansi in alloxan-induced diabetic rats. Br J Med Med Res 2014;4:4665-73.
Sandeep PM, Bovee TF, Sreejith K. Anti-androgenic activity of Nardostachys jatamansi DC and Tribulus terrestris L. and their beneficial effects on polycystic ovary syndrome–induced rat models. Metab Syndr Relat Disord 2015;13:248-54.
Yogeeswari P, Sriram D. Betulinic acid and its derivatives: a review on their biological properties. Curr Med Chem 2005;12:657-66.
Chandramu C, Manohar RD, Krupadanam DG, Dashavantha RV. Isolation, characterization and biological activity of betulinic acid and ursolic acid from Vitex negundo L. Phytother Res 2003;17:129-34.
DomÃnguez-Carmona DB, Escalante-Erosa F, GarcÃa-Sosa K, Ruiz-Pinell G, Gutierrez-Yapu D, Chan-Bacab MJ, et al. Antiprotozoal activity of betulinic acid derivatives. Phytomedicine 2010;17:379-82.
Pavlova NI, Savinova OV, Nikolaeva SN, Boreko EI, Flekhter OB. Antiviral activity of betulin, betulinic and betulonic acids against some enveloped and non-enveloped viruses. Fitoterapia 2003;74:489-92.
Mullauer FB, Kessler JH, Medema JP. Betulinic acid, a natural compound with potent anticancer effects. Anticancer Drugs 2010;2:215-27.
Yun Y, Han S, Park E, Yim D, Lee S, Lee CK, et al. Immunomodulatory activity of betulinic acid by producing pro-inflammatory cytokines and activation of macrophages. Arch Pharm Res 2003;26:1087-95.
Bori ID, Hung HY, Qian K, Chen CH, Morris-Natschke SL, Lee KH. Anti-AIDS agents 88 Anti-HIV conjugates of betulin and betulinic acid with AZT prepared via click chemistry. Tetrahedron Lett 2012;53:1987-9.
Manjamalai A, Narala Y, Haridas A, Grace VB. Antifungal, anti-inflammatory and GC-MS analysis of methanolic extract of Plectranthus amboinicus leaf. Int J Curr Pharm Res 2011;3:129-36.
Roy P, Das S, Bera T, Mondal S, Mukherjee A. Andrographolide nanoparticles in leishmaniasis: characterization and in vitro evaluations. Int J Nanomed 2010;5:1113-21.
Das S, Roy P, Mondal S, Bera T, Mukherjee A. One pot synthesis of gold nanoparticles and application in chemotherapy of wild and resistant type visceral leishmaniasis. Colloids Surf B 2013;107:27–34.
Ghosh S, Das S, De AK, Kar N, Bera T. Amphotericin B-loaded mannose modified poly (D, L-lactide-co-glycolide) polymeric nanoparticles for the treatment of visceral leishmaniasis: in vitro and in vivo approaches. RSC Adv 2017;7:29575-90.
Ghosh S, Kar N, Bera T. Oleanolic acid loaded poly lactic co-glycolic acidvitamin E TPGS nanoparticles for the treatment of Leishmania donovani infected visceral leishmaniasis. Int J Biol Macromol 2016;93:961-70.
Mondal S, Roy P, Das S, Halder A, Mukherjee A, Bera T. In vitro susceptibilities of wild and drug resistant Leishmania donovani amastigote stages to andrographolide nanoparticle: role of vitamin e derivative TPGS for nanoparticle efficacy. Plos One 2013;8:e81492.
Nwaka S, Hudson A. Innovative lead discovery strategies for tropical diseases, Nat Rev Drug Discovery 2006;5:941–55.
Singh SK, Bimal S, Narayan S, Jee C, Bimal D, Das P, et al. Leishmania donovani: assessment of leishmanicidal effects of herbal extracts obtained from plants in the visceral leishmaniasis endemic area of Bihar, India. Exp Parasitol 2011;127:552-8.
Naman CB, Gromovsky AD, Vela CM, Fletcher JN, Gupta G, Varikuti S, et al. Antileishmanial and cytotoxic activity of some highly oxidized abietane diterpenoids from the bald cypress, Taxodium distichum. J Nat Prod 2016;79:598-606.
Alakurtti S, Heiska T, Kiriazis A, Sacerdoti-Sierra N, Jaffe CL, Yli-Kauhaluoma J. Synthesis and anti-leishmanial activity of heterocyclic betulin derivatives. Bioorganic Med Chem 2010;18:1573-82.
Chowdhury S, Mukherjee T, Sengupta S, Chowdhury SR, Mukhopadhyay S, Majumder HK. Novel betulin derivatives as antileishmanial agents with mode of action targeting type IB DNA topoisomerase. Mol Pharmacol 2011;80:694-703.
Wert L, Alakurtti S, Corral MJ, Sánchez-Fortún S, Yli-Kauhaluoma J, Alunda JM. Toxicity of betulin derivatives and in vitro effect on promastigotes and amastigotes of Leishmania infantum and L. donovani. J Antibiot 2011;64:475.
Sousa MC, Varandas R, Santos RC, Santos-Rosa M, Alves V, Salvador JA. Antileishmanial activity of semisynthetic lupane triterpenoids betulin and betulinic acid derivatives: synergistic effects with miltefosine. PLOS One 2014;9:e89939.
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