BEHAVIORAL STUDIES OF WISTAR RATS IN ROTENONE INDUCED MODEL OF PARKINSON'S DISEASE
DOI:
https://doi.org/10.22159/ijpps.2017v9i11.21465Keywords:
Rotenone, Nil, Quercetin, Hesperidin, L-DOPA, Behavioural study, Biochemical parametersAbstract
Objective: The objective of the study was to determine the behavioral activities of Wistar rats induced with rotenone.
Methods: Thirty-six male Wistar rats were taken for the study and divided into six groups of six rats each. Group-I is the vehicle-treated, Group–II animals were induced with rotenone (3 mg/kg/bwt) by i. p. Group-III were co-treated with rotenone and L-DOPA (10 mg/kg/bwt) orally, Group-IV were co-treated with rotenone and quercetin (25 mg/kg/bwt) orally, Group-V were co-treated with rotenone and hesperidin (50 mg/kg/bwt) orally, Group-VI were treated with rotenone, quercetin and hesperidin in the same dosage regime for 60 d. The behavioural tests, such as open field test, ladder climbing test and hanging wire test were performed. The biochemical parameters such as urea, creatinine and activities of ALT and AST were also analysed.
Results: All data are expressed as the mean±SD. Disability was noted in the behaviour of rats induced with Parkinson's disease (PD). The deficits in behavioral activity were significantly changed when compared with an induced group (p<0.001) and biochemical parameters due to rotenone were significantly (p<0.001) restored by co-treatment with quercetin and hesperidin.
Conclusion: In our in vivo study, we have demonstrated the combination of quercetin and hesperidin to serve as neuroprotective compounds by improving the behavioral abnormalities and restoring the biochemical parameters. Hence, these powerful antioxidants may protect brain cells.
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Braak H. Gastric alpha-synuclein immune reactive inclusion in Meissner’s and Auerbach’s plexuses in cases staged for Parkinson’s disease-related brain pathology. Neuroscience 2006;396:167-72.
Ikemura M. Lewy body pathology involves cutaneous nerves. J Neuropathol Exp Neurol 2008;67:945-53.
Lebouvier T. The second brain and Parkinson’s disease. Eur J Neurosci 2009;30:735-41.
Hayes WJ. Handbook on pesticides; 1991. p. 1.
Lupescu, Adrian, Jilani, Kashif, Zbidah, Mohanad, et al. Induction of apoptotic erythrocyte death by rotenone. Toxicology 2012;300:132.
Gao HM, Liu B, Hong JS. Critical role for microglial NADPH oxidase in rotenone-induced degeneration of dopaminergic neurons. J Neurosci 2003;23:6181-7.
Freestone PS, Chung KK, Guatteo E, Mercuri NB, Nicholson LF, Lipski J, et al. Acute action of rotenone on nigral dopaminergic neurons-involvement of reactive oxygen species and disruption of Ca2+homeostasis. Eur J Neurosci 2009;30:1849-59.
Medicare D. Medicare Part D Program Information; 2014.
Sherer TB, Betarbet R, Testa CM, Seo BB, Richardso JR, Kim JH. Mechanism of toxicity in rotenone models of Parkinson’s disease. J Neurosci 2003;34:10756-64.
Yong R, Liu RW, Jiang H, Jiang Q, Feng J. Selective vulnerability of dopaminergic neurons to microtubule depolymerization. J Biol Chem 2005;280:34105-12.
Antunes M, Goes AT, Boeira SP, Prigol M, Sess CR. Protective effect of hesperidin in a model of Parkinson’s disease induced by 6-hydroxydopamine in aged mice. Nutrition 2014;30:1415-22.
Kuppusamy T, Jagadhesan N, Udaiyappan J, Tamilarasan M, Mustafa ME. The antioxidant and anti-inflammatory potential of hesperidin against 1-methyl-4-phenyl-1,2,3, 6-tetrahydropyridine-induced experimental. Int J Nutr Pharmacol Neurol Dis 2013;3:294-302.
Tamilselvam K, Braidy N, Manivasagam T, Essa MM, Prasad NR, Karthikeyan S, et al. Neuroprotective effects of hesperidin a plant flavanone on rotenone-induced oxidative stress and apoptosis in a cellular model for Parkinson’s disease. Oxid Med cell Longev 2013;10:11.
Heo HJ, Chang yong lee. Protective effects of quercetin and vitamin C against oxidative strees induced neurodegeneration. J Agric Food Chem 2004;52:7514-7.
Sing A, Naidu PS, Kulkarni SK. Quercetin potentiates L-DOPA reversal of drug-induced catalepsy in rats possible COMT/MAO inhibition. Pharmacology 2003;68:81-8.
Hastings TG, Lewis DA, Zigmond MJ. Role of oxidation in the neurotoxic effects of intrastriatal dopamine injection. Proc Natl Acad Sci USA 1996;93:1956-61.
Trushine E, Mcmurray CT. Oxidative stress and mitochondrial dysfunction in neurodegenerative diseases. Neuroscience 2007;145:1233-48.
Zoccarato F, Toscano P, Alexandre A. Dopamine-derived dopaminochrome promotes H2O2 release at mitochondria complex-1. J Biol Chem 2005;16:15587-94.
Walsh RN, Cummins RA. The open-field test: a critical review. Psychol Bull 1976;83:482-504.
Cummings BJ, Engesser-cesar C, Cadena G, Anderson AJ. Adaptation of a ladder bear walking task to assesss locomotor recovery in mice following spinal cord injury. Behav Brain Res 2007;177:232-41.
Tillerson JL, Miller GW. Grid performance test to measure behavioral impairment in the MPTP treated a mouse model of Parkinsonism. J Neurosci Meth 2003;123:189-200.
King J. The transferases-alanine and aspartate transaminases. In: Van D. ed Practical clinical enzymology; 1965. p. 191-208.
Natelson S, Scott ML, Beffa CA. Rapid method for the estimation of urea in biologic fluids. AM J Clin Pathol 1951;21:275.
Jaffe M. Uber den niederschag, welchen pikrinsaure in normalem harn erzevgt und uber eine neue rektion des kreatinins. Z Physiol Chem 1886;10:391-400.
Taetzsch T, Block ML. Pesticides,microglial Nox2 and parkinson’s disease. J Biochem Mol Toxicol 2013;27:137-49.
WC YN, Johnson SW. Dopamine oxidation facilitates rotenone-dependent potentiation of N-methyl-D-asparatate currents in rat substantia nigra dopamine neurons. Neuroscience 2011;195:138-44.
Betarbet R, Sherer TB, Di Monte DA, Greenamyre JT. Mechanistic approaches to Parkinson’s disease pathogenesis. Brain Pathol 2002;12:499-510.
Sindhu KM, Saravanan KS, Mohanokumar KP. Behavioral differences in a rotenone-induced hemi Parkinsonian rat model developed following in tranigral or median forebrain bundle infusion. Brain Res 2005;1051:25-34.
Schwarting RKW, Huston JP. The unilateral 6-hydroxydopamine lesion model in behavioral brain research, analysis of functional deficits, recovery and treatement. Prog Neurobiol 1996;50:275-33.
Cho JY, Kim Is, Jang YH, Kim AR, Lee SR. Protective effect of quercetin, a natural flavonoid against neuronal damage after transient global cerebral ischemia. Neurosci Bull 2006;404:330-5.
Zbarsky V, Datla KP, Parkar S, Rai DK, Aruoma OI, Dexter DT. Neuroprotective properties of the natural phenolic antioxidants curcumin and naringenin but not quercetin and tisetin in a 6-OHDA model of Parkinson’s disease. Free Radical Res 2005;39:1119-25.
Suryakantha Pany, Abhisekpal, Pratapkumar Saho. Neuroprotective effect of quercetin in neurotoxicity induced rats: the role of neuroinflammation in neurodegeneration. Asian J Pharm Clin Res 2014;7:152-6.
Muthukala B, Sivakumari K, Ashok K. Antioxidant and anti-inflammatory potential of quercetin. Int J Curr Pharm Res 2017;3:58-7.
Wagner C, Fachinelto R, Dalla Corte CI, Brito VB, Severo D, de oliveira Costa Dias G. Quercetin a glycoside form of quercetin prevents lipid peroxidation invitro. Brain Res 2006;1107:192-8.
Mehdi Mehdiadeh, Mohammad, Taghi Joghataei, Malliheh Nobakht, Roya Aryanapour. The beneficial effect of the flavonoid quercetin on behavioral changes in hemiparkinsonian rats. Basic Clin Neurosci 2009;1:30-2.
Noyce AJ, Bestwick JP, Silveira Moriyama L, Hawkes CH, Giavannoni G, Lees AJ et al. Meta-analysis of early non-motor features and risk factors for Parkinson disease. Ann Neurol 2012;72:893-901.
Matheus FC, Aguiar As Jr, Castro HA, Vilarinho JG, Ferreira J, Figueiredo CP. Neuroprotective effects of agmatine in mice infused with a single intranasal administration of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridin (MPTP). Behav Brain Res 2012;235:263-72.
Santiago RM, BArbieiro J, Lima MM, Dombrowski PA, Andreatini R, Vital MA, et al. Depressive like behavions alterations induced by intranigral MPTP, 6-OHDA, LPS and rotenone models of Parkinson’s disease are predominantly associated with serotonin and dopamine. Prog Neuro Psycho Pharmacol Biol Psychiatry 2010;34:1104-8.
Menze ET, Tadros MG, Abel-Tawab AM, Khalifa AE. Potential neuroprotective effects of hesperidin on 3-nitro propionic acid induced neurotoxicity in rats. Neurotoxicology 2012;33:1265-75.
Huang S, Tsai S, Lin J, WUC, Yen G. Cytoprotective effects of hesperidin and hesperidin against amyloid β-induced impairment of glucose transport through down-regulation of neuronal autophagy. Mol Nutr Food Res 2012;56:601-9.
Michelles, Antunes, Andre TR, Goes, Silvana P, Boeira, et al. Protective effect of hesperidin in a model of Parkinson’s disease induced by 6-hydroxydopamine in aged mice. Nutrition 2014;30:1415-22.
Li CY, Zug C, QU HC, Schluesener H, Zhang ZY. Hesperidin ameliorates behavioural impairments and neuropathology of transgenic APP/PSI mice. Behav Brain Res 2015:218:32-42.
Chandran Anusha, Thangarajan sumathi. Prodective role of Apigenin against the rotenone-induced model of Parkinson’s disease; Behavioral study. International J Toxicol Pharmacol Res 2016;8:79-82.
Dinesh T, Marhija, Aartig, Jagtap. Studies on the sensitivity of Zebrafish as a model organism for Parkinson’s disease; comparison with rat model. J Pharmacol Pharmacother 2014;5:39-46.
Priya Nagappan, Vijayalakshmi Krishnamurthy, Khadira Sereen. Investigation on the neuroproductive effects of hesperidin on behavioral activities in 6-OHDA induced Parkinson model. Int J Pharm Biol Sci 2014;5:570-7.
Singh N, Pillay V, Choonara YE. Advances in the treatement of Parkinson’s disease. Prog Neurobiol 2007;81:29-44.
Seibt KJ, Oliveira RL, Zimmermann FI, Cupiotti KM, Bogo MR, Ghisleni G, et al. Antipsychotic drugs prevent the motor hyperactivity induced by Psychotomimetic MK-801 in zebra fish [Danio nerio]. Behav Brain Res 2010;21:417-22.
He Y, Lee T, Leong SK. 6-hydroxydopamine induced apoptosis of dopaminergic cells in the rat substantia nigra. Brain Res 2000;858:163-6.
Michelle S, Antunes, Andre TR, Goes, Silvana P, Boeira, Marira Prigol, et al. Protective effect of hesperidin in a model of Parkinson’s disease induced by 6-hydroxydopamine in aged mice. Nutrition 2014;30:1415-22.
Bhagyasree P, Kalyani G. Neuroprotective effect of Anacardium Occidentale [Cashew apple fruit] against aluminum toxicity an experimental study on cognitive dysfunction and biochemical alterations in rats. Asian J Pharm Clin Res 2017;10:164-9.
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