ENHANCEMENT AND VALIDATION OF WOUND HEALING ACTIVITY WITH HERBAL GEL FORMULATED FROM SUB-FRACTION OF BUCHNANIA LANZAN SPRENG. BARK EXTRACT
Keywords:
Gel, Wound healing, Spreadability, Incision and excision wound modelAbstract
Objective: The scheme of the present study is to formulate and evaluate herbal gel prepared from sub- fraction of Buchnania lanazan bark extract to augment its wound healing activity.
Methods: Ethyl acetate fraction of Buchanania lanzan bark (Et- BLB) was sub-fractionated by column chromatography using (7:3 methanol: chloroform) solvent system, phytochemical test for Et- BLB and F1- 4 (ethyl acetate sub-fractions 1, 2, 3 & 4 of methanolic extract of BLB); in particular total flavonoid and phenolic content to demonstrate existence of anti-oxidant activity. In-vivo wound healing supporting study mediated by carrageenan induced paw edema as anti inflammatory activity. Herbal gel was formulated incorporating one of the active sub-fractions essentially F4 in two concentrations (1% & 5%). Carbopol 934, propylene glycol 400, methyl paraben & quantity sufficient distilled water was used to prepare herbal gel; then tri-ethanolamine was added to get neutral pH (6.8 - 7) & gel was evaluated for its spreadability, pH, colour, consistency & appearance. Excision & incision models were used to validate and to review enhancement competence of wound healing activity; employing percent wound contraction & tensile strength of wounded skin as parameters. Reference standard used was framycetin sulphate cream.
Results: Total phenolic and flavonoid content of F4 was showing highest flavonoid and phenolic content 83.3 mg/g and 77.8 mg/g respectively. Prepared gel was reddish brown in colour with satisfying spreadability, acceptable appearance along with homogeneity and negligible irritation. Excision and incision animal wound model, groups treated with F4 5 % gel and reference standard exhibited significant increase in percentage of wound contraction as well as up growth in tensile strength with 177 g (P<0.05) and 181.2 g (P<0.01) respectively.
Conclusion: Data from both the models revealed that 5% gel of F4 showed significant & enhanced wound healing.
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References
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